Heat Shock Factor I Antibody, clone 10H8 | EMD Millipore MAB88078 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Heat Shock Factor I Antibody, clone 10H8

catalog :

MAB88078

quantity :

50 µg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

10H8

The same clone is also sold as:

Abnova: MAB2285, MAB16136, MAB16307
StressMarq Biosciences: SMc-118D
Enzo Life Sciences: ADI-SPA-950-D, ADI-SPA-950-F
OriGene: TA326414
Invitrogen: MA5-27688
Santa Cruz Biotechnology: sc-13516, sc-13516 X
Rockland Immunochemicals: 200-501-F50
LifeSpan Biosciences: LS-C227272, LS-C227275, LS-C227262, LS-C227265, LS-C152958, LS-C227274, LS-C227271, LS-B3707, LS-C227263, LS-C227273, LS-C227261, LS-C227264, LS-C227260, LS-C227259, LS-B8141, LS-C227269, LS-C227268
Abcam: ab61382

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

product information

Catalog Number :

MAB88078

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Heat Shock Factor I Antibody, clone 10H8

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q00613

Host Name :

Rat

Antigen :

Heat Shock Factor I

Clone :

10H8

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Heat Shock Factor I Antibody, clone 10H8

Cross Reactivity :

Human;Mouse;Monkey

ALT Names :

HSFI

Immunogen :

Full length recombinanat mouse HSF1 protein (aa 1-503).

Specificity :

MAB88078 recognizes a protein of 70-85 kDa (depending upon the souce and state of cells), identified as Heat Shock Factor 1 (HSF1). Reactivity with delection mutants suggests that the epitope of MAB88078 spans in the aa 378-395 of mouse HSF1. The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediate by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosophila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. The phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes. Cellular Localization: Cytoplasmic domain

Package Size :

50 µg

Uses :

ELISA;Immunofluorescence;Immunoprecipitation;Western Blotting

Storage :

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.