Cytomegalovirus Antibody, early, clone 5A8.2 | EMD Millipore MAB8140 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cytomegalovirus Antibody, early, clone 5A8.2

catalog :

MAB8140

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A8.2

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 6

Reference
Li G, Rak M, Nguyen C, Umashankar M, Goodrum F, Kamil J. An epistatic relationship between the viral protein kinase UL97 and the UL133-UL138 latency locus during the human cytomegalovirus lytic cycle. J Virol. 2014;88:6047-60 pubmed publisher
Caffarelli N, Fehr A, Yu D. Cyclin A degradation by primate cytomegalovirus protein pUL21a counters its innate restriction of virus replication. PLoS Pathog. 2013;9:e1003825 pubmed publisher
Van Domselaar R, de Poot S, Remmerswaal E, Lai K, Ten Berge I, Bovenschen N. Granzyme M targets host cell hnRNP K that is essential for human cytomegalovirus replication. Cell Death Differ. 2013;20:419-29 pubmed publisher
Fehr A, Gualberto N, Savaryn J, Terhune S, Yu D. Proteasome-dependent disruption of the E3 ubiquitin ligase anaphase-promoting complex by HCMV protein pUL21a. PLoS Pathog. 2012;8:e1002789 pubmed publisher
Perng Y, Qian Z, Fehr A, Xuan B, Yu D. The human cytomegalovirus gene UL79 is required for the accumulation of late viral transcripts. J Virol. 2011;85:4841-52 pubmed publisher
Fehr A, Yu D. Human cytomegalovirus early protein pUL21a promotes efficient viral DNA synthesis and the late accumulation of immediate-early transcripts. J Virol. 2011;85:663-74 pubmed publisher

product information

Catalog Number :

MAB8140

Subcategory :

Infectious Diseases

Product Name :

Anti-Cytomegalovirus Antibody, early, clone 5A8.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

Cytomegalovirus

Clone :

5A8.2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Cytomegalovirus Antibody, early, clone 5A8.2

Cross Reactivity :

Human

ALT Names :

CMV

Immunogen :

Affinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).

Specificity :

Reacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours. With CMV the antigens expressed at different times are listed as: Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD. Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis. Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Package Size :

100 µg

Uses :

Immunofluorescence;Western Blotting;Immunohistochemistry;Immunoprecipitation

Storage :

Maintain at +4°C for up to 12 months