This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Cytomegalovirus Antibody, clone 8B1.2
catalog :
MAB810R
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8B1.2
reactivity :
human, Human cytomegalovirus
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 31
Published Application/Species/Sample/DilutionReference
  • western blot; Human cytomegalovirus; loading ...; fig 1b
Botto S, Abraham J, Mizuno N, Pryke K, Gall B, Landais I, et al. Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome. MBio. 2019;10: pubmed publisher
  • western blot; human; fig 1A
Kim Y, Kim E, Kim Y, Lee M, Kwon K, Kim K, et al. Consecutive Inhibition of ISG15 Expression and ISGylation by Cytomegalovirus Regulators. PLoS Pathog. 2016;12:e1005850 pubmed publisher
  • western blot; Human cytomegalovirus; fig 8
Harwardt T, Lukas S, Zenger M, Reitberger T, Danzer D, Übner T, et al. Human Cytomegalovirus Immediate-Early 1 Protein Rewires Upstream STAT3 to Downstream STAT1 Signaling Switching an IL6-Type to an IFNγ-Like Response. PLoS Pathog. 2016;12:e1005748 pubmed publisher
  • western blot; human; loading ...; fig 5c
Kim Y, Ahn J. Positive role of promyelocytic leukemia protein in type I interferon response and its regulation by human cytomegalovirus. PLoS Pathog. 2015;11:e1004785 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
Eifler M, Uecker R, Weisbach H, Bogdanow B, Richter E, König L, et al. PUL21a-Cyclin A2 interaction is required to protect human cytomegalovirus-infected cells from the deleterious consequences of mitotic entry. PLoS Pathog. 2014;10:e1004514 pubmed publisher
  • immunohistochemistry; Human cytomegalovirus; 1:1000; fig 2
Libard S, Popova S, Amini R, Kärjä V, Pietiläinen T, Hämäläinen K, et al. Human cytomegalovirus tegument protein pp65 is detected in all intra- and extra-axial brain tumours independent of the tumour type or grade. PLoS ONE. 2014;9:e108861 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:500
Yaiw K, Ovchinnikova O, Taher C, Mohammad A, Davoudi B, Shlyakhto E, et al. High prevalence of human cytomegalovirus in carotid atherosclerotic plaques obtained from Russian patients undergoing carotid endarterectomy. Herpesviridae. 2013;4:3 pubmed publisher
  • western blot; human
Mücke K, Paulus C, Bernhardt K, Gerrer K, Schön K, Fink A, et al. Human cytomegalovirus major immediate early 1 protein targets host chromosomes by docking to the acidic pocket on the nucleosome surface. J Virol. 2014;88:1228-48 pubmed publisher
van Senten J, Bebelman M, Fan T, Heukers R, Bergkamp N, van Gasselt P, et al. The human cytomegalovirus-encoded G protein-coupled receptor UL33 exhibits oncomodulatory properties. J Biol Chem. 2019;294:16297-16308 pubmed publisher
Lee M, Kim Y, Kim Y, Han T, Milbradt J, Marschall M, et al. Transmembrane Protein pUL50 of Human Cytomegalovirus Inhibits ISGylation by Downregulating UBE1L. J Virol. 2018;92: pubmed publisher
Gergen J, Coulon F, Creneguy A, Elain Duret N, Gutierrez A, Pinkenburg O, et al. Multiplex CRISPR/Cas9 system impairs HCMV replication by excising an essential viral gene. PLoS ONE. 2018;13:e0192602 pubmed publisher
de Wit R, Mujić Delić A, van Senten J, Fraile Ramos A, Siderius M, Smit M. Human cytomegalovirus encoded chemokine receptor US28 activates the HIF-1α/PKM2 axis in glioblastoma cells. Oncotarget. 2016;7:67966-67985 pubmed publisher
Costa H, Xu X, Overbeek G, Vasaikar S, Patro C, Kostopoulou O, et al. Human cytomegalovirus may promote tumour progression by upregulating arginase-2. Oncotarget. 2016;7:47221-47231 pubmed publisher
Le Trilling V, Megger D, Katschinski B, Landsberg C, Rückborn M, Tao S, et al. Broad and potent antiviral activity of the NAE inhibitor MLN4924. Sci Rep. 2016;6:19977 pubmed publisher
Esteso G, Luzón E, Sarmiento E, Gómez Caro R, Steinle A, Murphy G, et al. Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA. J Immunol. 2014;193:1344-52 pubmed publisher
Yan Z, Wang L, Dennis J, Doern C, Baker J, Park J. Clinical significance of isolated cytomegalovirus-infected gastrointestinal cells. Int J Surg Pathol. 2014;22:492-8 pubmed publisher
Wiebusch L, Hagemeier C. Use of 5-ethynyl-2'-deoxyuridine labelling and flow cytometry to study cell cycle-dependent regulation of human cytomegalovirus gene expression. Methods Mol Biol. 2014;1119:123-32 pubmed publisher
Huang T, Lee J, Cheng S. No evidence of association between human cytomegalovirus infection and papillary thyroid cancer. World J Surg Oncol. 2014;12:41 pubmed publisher
Yaiw K, Mohammad A, Taher C, Wilhelmi V, Davoudi B, Strååt K, et al. Human cytomegalovirus induces upregulation of arginase II: possible implications for vasculopathies. Basic Res Cardiol. 2014;109:401 pubmed publisher
Pereira L, Petitt M, Fong A, Tsuge M, Tabata T, Fang Hoover J, et al. Intrauterine growth restriction caused by underlying congenital cytomegalovirus infection. J Infect Dis. 2014;209:1573-84 pubmed publisher
Khan Z, Yaiw K, Wilhelmi V, Lam H, Rahbar A, Stragliotto G, et al. Human cytomegalovirus immediate early proteins promote degradation of connexin 43 and disrupt gap junction communication: implications for a role in gliomagenesis. Carcinogenesis. 2014;35:145-54 pubmed publisher
Djaoud Z, David G, Bressollette C, Willem C, Rettman P, Gagne K, et al. Amplified NKG2C+ NK cells in cytomegalovirus (CMV) infection preferentially express killer cell Ig-like receptor 2DL: functional impact in controlling CMV-infected dendritic cells. J Immunol. 2013;191:2708-16 pubmed publisher
Schleiss M, Choi K, Anderson J, Mash J, Wettendorff M, Mossman S, et al. Glycoprotein B (gB) vaccines adjuvanted with AS01 or AS02 protect female guinea pigs against cytomegalovirus (CMV) viremia and offspring mortality in a CMV-challenge model. Vaccine. 2014;32:2756-62 pubmed publisher
Omoto S, Mocarski E. Cytomegalovirus UL91 is essential for transcription of viral true late (?2) genes. J Virol. 2013;87:8651-64 pubmed publisher
Kim Y, Lee S, Kim S, Kim D, Ahn J, Ahn K. Human cytomegalovirus clinical strain-specific microRNA miR-UL148D targets the human chemokine RANTES during infection. PLoS Pathog. 2012;8:e1002577 pubmed publisher
Melnick M, Sedghizadeh P, Allen C, Jaskoll T. Human cytomegalovirus and mucoepidermoid carcinoma of salivary glands: cell-specific localization of active viral and oncogenic signaling proteins is confirmatory of a causal relationship. Exp Mol Pathol. 2012;92:118-25 pubmed publisher
Ehlinger E, Webster E, Kang H, Cangialose A, Simmons A, Barbas K, et al. Maternal cytomegalovirus-specific immune responses and symptomatic postnatal cytomegalovirus transmission in very low-birth-weight preterm infants. J Infect Dis. 2011;204:1672-82 pubmed publisher
Ahlqvist J, Mocarski E. Cytomegalovirus UL103 controls virion and dense body egress. J Virol. 2011;85:5125-35 pubmed publisher
Lucas K, Bao L, Bruggeman R, Dunham K, Specht C. The detection of CMV pp65 and IE1 in glioblastoma multiforme. J Neurooncol. 2011;103:231-8 pubmed publisher
Millard A, Haberli L, Sinzger C, Ghielmetti M, Schneider M, Bossart W, et al. Efficiency of porcine endothelial cell infection with human cytomegalovirus depends on both virus tropism and endothelial cell vascular origin. Xenotransplantation. 2010;17:274-87 pubmed publisher
Chen I, Lai Y, Wu C, Chang Y, Chu C, Tsai I, et al. Immune impairment in patients with terminal cancers: influence of cancer treatments and cytomegalovirus infection. Cancer Immunol Immunother. 2010;59:323-34 pubmed publisher
product information
Catalog Number :
MAB810R
Subcategory :
Infectious Diseases
Product Name :
Anti-Cytomegalovirus Antibody, clone 8B1.2
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
Cytomegalovirus
Clone :
8B1.2
Conjugate :
Purified
Isotype :
IgG2a
Product Description :
Anti-Cytomegalovirus Antibody, clone 8B1.2
Cross Reactivity :
Human
Background :
Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that belongs to the herpes adenovirus family. The viral life cycle takes approximately seventy two hours. After the initial fusion of the viral envelope with the plasma membrane of the cell, the encapisidated virus particle is released into the cytoplasm and within minutes, transits to the nucleus. Via active transport through the nuclear pore, the capsid gains entry and viral DNA is deposited. Viral gene expression then occurs in a temporally regulated manner, first with expression of the immediate early genes, followed by the early genes, then, after viral replication has commenced, the late genes. All of the immediate early proteins have been shown to be transactivators, with IE1-72 and IE2-86 being the most well characterized. These genes regulate the expression of factors required for virus replication.
ALT Names :
CMV
Immunogen :
Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Specificity :
Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.
Package Size :
100 μg
Uses :
ELISA;Flow Cytometry;Immunofluorescence;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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