This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Vesicular Glutamate Transporter 2 Antibody
catalog :
MAB5504
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8G9.2

The same clone is also sold as:
reactivity :
eastern gray squirrel, white-toothed pygmy shrew, grey mouse lemur, Saimiri boliviensis peruviensis, African green monkey, Northern elephant seal, Tree pangolin, small-eared galago, human, mouse, rat, domestic sheep, rhesus macaque
application :
western blot, immunohistochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 66
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig s1a
Otsu Y, Darcq E, Pietrajtis K, Matyas F, Schwartz E, Bessaih T, et al. Control of aversion by glycine-gated GluN1/GluN3A NMDA receptors in the adult medial habenula. Science. 2019;366:250-254 pubmed publisher
  • immunohistochemistry - frozen section; grey mouse lemur; 1:5000; loading ...
Saraf M, Balaram P, Pifferi F, Kennedy H, Kaas J. The sensory thalamus and visual midbrain in mouse lemurs. J Comp Neurol. 2019;527:2599-2611 pubmed publisher
  • immunohistochemistry; Tree pangolin; 1:4000; loading ...; fig 6c
Imam A, Bhagwandin A, Ajao M, Ihunwo A, Manger P. The brain of the tree pangolin (Manis tricuspis). IV. The hippocampal formation. J Comp Neurol. 2019;527:2393-2412 pubmed publisher
  • immunohistochemistry; grey mouse lemur; 1:5000; loading ...; fig 2a
Saraf M, Balaram P, Pifferi F, Gămănuţ R, Kennedy H, Kaas J. Architectonic features and relative locations of primary sensory and related areas of neocortex in mouse lemurs. J Comp Neurol. 2019;527:625-639 pubmed publisher
  • immunohistochemistry - free floating section; rat; loading ...; fig 3a
  • immunohistochemistry - free floating section; mouse; loading ...; fig 3b
Lauer S, Lenschow C, Brecht M. Sexually selected size differences and conserved sexual monomorphism of genital cortex. J Comp Neurol. 2017;525:2706-2718 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:3000; loading ...; fig 3a
Hayashi K, Furuya A, Sakamaki Y, Akagi T, Shinoda Y, Sadakata T, et al. The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination. PLoS ONE. 2017;12:e0173175 pubmed publisher
  • western blot; mouse; loading ...; tbl 1
Cao M, Wu Y, Ashrafi G, McCartney A, Wheeler H, Bushong E, et al. Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons. Neuron. 2017;93:882-896.e5 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 4p
Subashini C, Dhanesh S, Chen C, Riya P, Meera V, Divya T, et al. Wnt5a is a crucial regulator of neurogenesis during cerebellum development. Sci Rep. 2017;7:42523 pubmed publisher
  • immunohistochemistry; Northern elephant seal; 0.2 ug/ml; loading ...; tbl 1
Turner E, Sawyer E, Kaas J. Optic nerve, superior colliculus, visual thalamus, and primary visual cortex of the northern elephant seal (Mirounga angustirostris) and California sea lion (Zalophus californianus). J Comp Neurol. 2017;525:2109-2132 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 7q
Lizen B, Hutlet B, Bissen D, Sauvegarde D, Hermant M, Ahn M, et al. HOXA5 localization in postnatal and adult mouse brain is suggestive of regulatory roles in postmitotic neurons. J Comp Neurol. 2017;525:1155-1175 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 2
Laramée M, Smolders K, Hu T, Bronchti G, Boire D, Arckens L. Congenital Anophthalmia and Binocular Neonatal Enucleation Differently Affect the Proteome of Primary and Secondary Visual Cortices in Mice. PLoS ONE. 2016;11:e0159320 pubmed publisher
  • immunohistochemistry; mouse; 1:300; loading ...; fig 4h
Wang Y, Hersheson J, López D, Hammer M, Liu Y, Lee K, et al. Defects in the CAPN1 Gene Result in Alterations in Cerebellar Development and Cerebellar Ataxia in Mice and Humans. Cell Rep. 2016;16:79-91 pubmed publisher
  • immunohistochemistry - free floating section; domestic sheep; 1:500
Merkley C, Coolen L, Goodman R, Lehman M. Evidence for Changes in Numbers of Synaptic Inputs onto KNDy and GnRH Neurones during the Preovulatory LH Surge in the Ewe. J Neuroendocrinol. 2015;27:624-35 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s4b
Wei P, Liu N, Zhang Z, Liu X, Tang Y, He X, et al. Processing of visually evoked innate fear by a non-canonical thalamic pathway. Nat Commun. 2015;6:6756 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 3f
Lee D, Jeong J, Chun S, Chua S, Jo Y. Interplay between glucose and leptin signalling determines the strength of GABAergic synapses at POMC neurons. Nat Commun. 2015;6:6618 pubmed publisher
  • western blot; rat; 1:2000; fig s3
Zhang S, Qi J, Li X, Wang H, Britt J, Hoffman A, et al. Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons. Nat Neurosci. 2015;18:386-92 pubmed publisher
  • immunohistochemistry; mouse; 1:500
White J, Arancillo M, Stay T, George Jones N, Levy S, Heck D, et al. Cerebellar zonal patterning relies on Purkinje cell neurotransmission. J Neurosci. 2014;34:8231-45 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:500
Manca P, Mameli O, Caria M, Torrej n Escribano B, Blasi J. Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration. Neuroscience. 2014;266:102-15 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:5000
Sadakata T, Kakegawa W, Shinoda Y, Hosono M, Katoh Semba R, Sekine Y, et al. CAPS1 deficiency perturbs dense-core vesicle trafficking and Golgi structure and reduces presynaptic release probability in the mouse brain. J Neurosci. 2013;33:17326-34 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:500
Reeber S, Loeschel C, Franklin A, Sillitoe R. Establishment of topographic circuit zones in the cerebellum of scrambler mutant mice. Front Neural Circuits. 2013;7:122 pubmed publisher
  • immunohistochemistry; rhesus macaque; 1:2,000
Cerkevich C, Qi H, Kaas J. Thalamic input to representations of the teeth, tongue, and face in somatosensory area 3b of macaque monkeys. J Comp Neurol. 2013;521:3954-71 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500
Puglisi F, Vanni V, Ponterio G, Tassone A, Sciamanna G, Bonsi P, et al. Torsin A Localization in the Mouse Cerebellar Synaptic Circuitry. PLoS ONE. 2013;8:e68063 pubmed publisher
  • immunohistochemistry - frozen section; Saimiri boliviensis peruviensis
  • immunohistochemistry - frozen section; small-eared galago
Liao C, Gharbawie O, Qi H, Kaas J. Cortical connections to single digit representations in area 3b of somatosensory cortex in squirrel monkeys and prosimian galagos. J Comp Neurol. 2013;521:3768-90 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000
Ebner B, Ingram M, Barnes J, Duvick L, Frisch J, Clark H, et al. Purkinje cell ataxin-1 modulates climbing fiber synaptic input in developing and adult mouse cerebellum. J Neurosci. 2013;33:5806-20 pubmed publisher
  • western blot; rat
Zha Y, Wang Y, Deng Y, Zhang R, Tan X, Yuan W, et al. Exercise training lowers the enhanced tonically active glutamatergic input to the rostral ventrolateral medulla in hypertensive rats. CNS Neurosci Ther. 2013;19:244-51 pubmed publisher
  • immunohistochemistry - frozen section; small-eared galago; 1:5000
  • western blot; small-eared galago
Baldwin M, Balaram P, Kaas J. Projections of the superior colliculus to the pulvinar in prosimian galagos (Otolemur garnettii) and VGLUT2 staining of the visual pulvinar. J Comp Neurol. 2013;521:1664-82 pubmed publisher
  • immunohistochemistry - frozen section; African green monkey; 1:5000
Marion R, Li K, Purushothaman G, Jiang Y, Casagrande V. Morphological and neurochemical comparisons between pulvinar and V1 projections to V2. J Comp Neurol. 2013;521:813-32 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:4000
  • immunohistochemistry - frozen section; rhesus macaque; 1:4000
Garcia Marin V, Ahmed T, Afzal Y, Hawken M. Distribution of vesicular glutamate transporter 2 (VGluT2) in the primary visual cortex of the macaque and human. J Comp Neurol. 2013;521:130-51 pubmed publisher
  • immunohistochemistry - frozen section; white-toothed pygmy shrew; 1:1000
Naumann R, Anjum F, Roth Alpermann C, Brecht M. Cytoarchitecture, areas, and neuron numbers of the Etruscan shrew cortex. J Comp Neurol. 2012;520:2512-30 pubmed publisher
  • immunohistochemistry; mouse; 1:500
Kadriu B, Guidotti A, Chen Y, Grayson D. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012;520:1951-64 pubmed publisher
  • immunohistochemistry - frozen section; eastern gray squirrel; 1:5000
Baldwin M, Wong P, Reed J, Kaas J. Superior colliculus connections with visual thalamus in gray squirrels (Sciurus carolinensis): evidence for four subdivisions within the pulvinar complex. J Comp Neurol. 2011;519:1071-94 pubmed publisher
  • immunohistochemistry - frozen section; rhesus macaque; 1:2000
Qi H, Gharbawie O, Wong P, Kaas J. Cell-poor septa separate representations of digits in the ventroposterior nucleus of the thalamus in monkeys and prosimian galagos. J Comp Neurol. 2011;519:738-58 pubmed publisher
  • immunohistochemistry; rat; 1:500
  • western blot; rat; 1:500
Griffin G, Ferri Kolwicz S, Reyes B, Van Bockstaele E, Flanagan Cato L. Ovarian hormone-induced reorganization of oxytocin-labeled dendrites and synapses lateral to the hypothalamic ventromedial nucleus in female rats. J Comp Neurol. 2010;518:4531-45 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:400
Issa A, Zhan W, Sieck G, Mantilla C. Neuregulin-1 at synapses on phrenic motoneurons. J Comp Neurol. 2010;518:4213-25 pubmed publisher
  • immunohistochemistry; rat
Gómez Nieto R, Rubio M. A bushy cell network in the rat ventral cochlear nucleus. J Comp Neurol. 2009;516:241-63 pubmed publisher
  • immunohistochemistry - frozen section; eastern gray squirrel; 1:2000
Wong P, Gharbawie O, Luethke L, Kaas J. Thalamic connections of architectonic subdivisions of temporal cortex in grey squirrels (Sciurus carolinensis). J Comp Neurol. 2008;510:440-61 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:5000
Wässle H, Regus Leidig H, Haverkamp S. Expression of the vesicular glutamate transporter vGluT2 in a subset of cones of the mouse retina. J Comp Neurol. 2006;496:544-55 pubmed
Shakya M, Shrestha P, Briski K. Hindbrain 5'-Adenosine Monophosphate-activated Protein Kinase Mediates Short-term Food Deprivation Inhibition of the Gonadotropin-releasing Hormone-Luteinizing Hormone Axis: Role of Nitric Oxide. Neuroscience. 2018;383:46-59 pubmed publisher
Ferrai C, Torlai Triglia E, Risner Janiczek J, Rito T, Rackham O, de Santiago I, et al. RNA polymerase II primes Polycomb-repressed developmental genes throughout terminal neuronal differentiation. Mol Syst Biol. 2017;13:946 pubmed publisher
Yin W, Sun Z, Mendenhall J, Walker D, Riha P, Bezner K, et al. Expression of Vesicular Glutamate Transporter 2 (vGluT2) on Large Dense-Core Vesicles within GnRH Neuroterminals of Aging Female Rats. PLoS ONE. 2015;10:e0129633 pubmed publisher
Sauvageau M, Goff L, Lodato S, Bonev B, Groff A, Gerhardinger C, et al. Multiple knockout mouse models reveal lincRNAs are required for life and brain development. elife. 2013;2:e01749 pubmed publisher
Bryant K, Suwyn C, Reding K, Smiley J, Hackett T, Preuss T. Evidence for ape and human specializations in geniculostriate projections from VGLUT2 immunohistochemistry. Brain Behav Evol. 2012;80:210-21 pubmed publisher
Zhang J, Xi M, Fung S, Sampogna S, Chase M. Projections from the central nucleus of the amygdala to the nucleus pontis oralis in the rat: an anterograde labeling study. Neurosci Lett. 2012;525:157-62 pubmed publisher
Aizawa H, Kobayashi M, Tanaka S, Fukai T, Okamoto H. Molecular characterization of the subnuclei in rat habenula. J Comp Neurol. 2012;520:4051-66 pubmed publisher
Affleck V, Coote J, Pyner S. The projection and synaptic organisation of NTS afferent connections with presympathetic neurons, GABA and nNOS neurons in the paraventricular nucleus of the hypothalamus. Neuroscience. 2012;219:48-61 pubmed publisher
Gupta D, Hubscher C. Estradiol treatment prevents injury induced enhancement in spinal cord dynorphin expression. Front Physiol. 2012;3:28 pubmed publisher
Barnes J, Ebner B, Duvick L, Gao W, Chen G, Orr H, et al. Abnormalities in the climbing fiber-Purkinje cell circuitry contribute to neuronal dysfunction in ATXN1[82Q] mice. J Neurosci. 2011;31:12778-89 pubmed publisher
Gebre S, Reeber S, Sillitoe R. Parasagittal compartmentation of cerebellar mossy fibers as revealed by the patterned expression of vesicular glutamate transporters VGLUT1 and VGLUT2. Brain Struct Funct. 2012;217:165-80 pubmed publisher
GEHRKING K, Andresen J, Duvick L, Lough J, Zoghbi H, Orr H. Partial loss of Tip60 slows mid-stage neurodegeneration in a spinocerebellar ataxia type 1 (SCA1) mouse model. Hum Mol Genet. 2011;20:2204-12 pubmed publisher
Reeber S, Gebre S, Sillitoe R. Fluorescence mapping of afferent topography in three dimensions. Brain Struct Funct. 2011;216:159-69 pubmed publisher
Gómez Nieto R, Rubio M. Ultrastructure, synaptic organization, and molecular components of bushy cell networks in the anteroventral cochlear nucleus of the rhesus monkey. Neuroscience. 2011;179:188-207 pubmed publisher
Fung S, Xi M, Zhang J, Torterolo P, Sampogna S, Morales F, et al. Projection neurons from the central nucleus of the amygdala to the nucleus pontis oralis. J Neurosci Res. 2011;89:429-36 pubmed publisher
Fyk Kolodziej B, Shimano T, Gong T, Holt A. Vesicular glutamate transporters: spatio-temporal plasticity following hearing loss. Neuroscience. 2011;178:218-39 pubmed publisher
Pose I, Sampogna S, Chase M, Morales F. Nitrergic ventro-medial medullary neurons activated during cholinergically induced active (rapid eye movement) sleep in the cat. Neuroscience. 2011;172:246-55 pubmed publisher
Duvick L, Barnes J, Ebner B, Agrawal S, ANDRESEN M, Lim J, et al. SCA1-like disease in mice expressing wild-type ataxin-1 with a serine to aspartic acid replacement at residue 776. Neuron. 2010;67:929-35 pubmed publisher
Massie A, Schallier A, Vermoesen K, Arckens L, Michotte Y. Biphasic and bilateral changes in striatal VGLUT1 and 2 protein expression in hemi-Parkinson rats. Neurochem Int. 2010;57:111-8 pubmed publisher
Wong P, Kaas J. Architectonic subdivisions of neocortex in the Galago (Otolemur garnetti). Anat Rec (Hoboken). 2010;293:1033-69 pubmed publisher
Sun Q. Experience-dependent intrinsic plasticity in interneurons of barrel cortex layer IV. J Neurophysiol. 2009;102:2955-73 pubmed publisher
Chuhma N, Choi W, Mingote S, Rayport S. Dopamine neuron glutamate cotransmission: frequency-dependent modulation in the mesoventromedial projection. Neuroscience. 2009;164:1068-83 pubmed publisher
Fattorini G, Verderio C, Melone M, Giovedi S, Benfenati F, Matteoli M, et al. VGLUT1 and VGAT are sorted to the same population of synaptic vesicles in subsets of cortical axon terminals. J Neurochem. 2009;110:1538-46 pubmed publisher
Wong P, Kaas J. An architectonic study of the neocortex of the short-tailed opossum (Monodelphis domestica). Brain Behav Evol. 2009;73:206-28 pubmed publisher
Wong P, Kaas J. Architectonic subdivisions of neocortex in the tree shrew (Tupaia belangeri). Anat Rec (Hoboken). 2009;292:994-1027 pubmed publisher
Hackett T, de la Mothe L. Regional and laminar distribution of the vesicular glutamate transporter, VGluT2, in the macaque monkey auditory cortex. J Chem Neuroanat. 2009;38:106-16 pubmed publisher
Wong P, Kaas J. Architectonic subdivisions of neocortex in the gray squirrel (Sciurus carolinensis). Anat Rec (Hoboken). 2008;291:1301-33 pubmed publisher
Jeong J, Kim H, Hwang S, Park J, Lee B. Region- and neuronal phenotype-specific expression of NELL2 in the adult rat brain. Mol Cells. 2008;26:186-92 pubmed
Sadakata T, Kakegawa W, Mizoguchi A, Washida M, Katoh Semba R, Shutoh F, et al. Impaired cerebellar development and function in mice lacking CAPS2, a protein involved in neurotrophin release. J Neurosci. 2007;27:2472-82 pubmed
product information
Catalog Number :
MAB5504
Subcategory :
Neuroscience
Product Name :
Anti-Vesicular Glutamate Transporter 2 Antibody
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q9JI12
Host Name :
Mouse
Antigen :
Vesicular Glutamate Transporter 2
Clone :
8G9.2
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Vesicular Glutamate Transporter 2 Antibody
Cross Reactivity :
Human;Mouse;Rat
Background :
Vesicular Glutamate Transporter 2 (VGLUT2) is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity.
ALT Names :
VGLUT2
Immunogen :
Recombinant protein from rat VGLUT2.
Specificity :
Reacts with VGLUT2 (Vesicular Glutamate Transporter 2).
Package Size :
100 μg
Uses :
Western Blotting;Immunohistochemistry
Storage :
Stable for 1 year at from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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