Vesicular Glutamate Transporter 1 Antibody | EMD Millipore MAB5502 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Vesicular Glutamate Transporter 1 Antibody

catalog :

MAB5502

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C10.2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

citations: 30

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; loading ...; fig 4g	Zhong L, Xu Y, Zhuo R, Wang T, Wang K, Huang R, et al. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model. Nat Commun. 2019;10:1365 pubmed publisher
immunocytochemistry; mouse; 1:500; loading ...; fig 1f	Young F, Keruzore M, Nan X, Gennet N, Bellefroid E, Li M. The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1. Proc Natl Acad Sci U S A. 2017;114:E5599-E5607 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 4v	Subashini C, Dhanesh S, Chen C, Riya P, Meera V, Divya T, et al. Wnt5a is a crucial regulator of neurogenesis during cerebellum development. Sci Rep. 2017;7:42523 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3a	Stuart K, King A, Fernandez Martos C, Dittmann J, Summers M, Vickers J. Mid-life environmental enrichment increases synaptic density in CA1 in a mouse model of A?-associated pathology and positively influences synaptic and cognitive health in healthy ageing. J Comp Neurol. 2017;525:1797-1810 pubmed publisher
western blot; rat; 1:1000; fig 6a	McQuail J, Beas B, Kelly K, Simpson K, Frazier C, Setlow B, et al. NR2A-Containing NMDARs in the Prefrontal Cortex Are Required for Working Memory and Associated with Age-Related Cognitive Decline. J Neurosci. 2016;36:12537-12548 pubmed
immunohistochemistry - frozen section; mouse; 1:200; fig 5r	Divya T, Lalitha S, Parvathy S, Subashini C, Sanalkumar R, Dhanesh S, et al. Regulation of Tlx3 by Pax6 is required for the restricted expression of ChrnÎ±3 in Cerebellar Granule Neuron progenitors during development. Sci Rep. 2016;6:30337 pubmed publisher
western blot; mouse; 1:2000; loading ...; fig 2	Laramée M, Smolders K, Hu T, Bronchti G, Boire D, Arckens L. Congenital Anophthalmia and Binocular Neonatal Enucleation Differently Affect the Proteome of Primary and Secondary Visual Cortices in Mice. PLoS ONE. 2016;11:e0159320 pubmed publisher
western blot; mouse; fig 1	Uda Y, Xu S, Matsumura T, Takei Y. P2Y4 Nucleotide Receptor in Neuronal Precursors Induces Glutamatergic Subtype Markers in Their Descendant Neurons. Stem Cell Reports. 2016;6:474-482 pubmed publisher
western blot; mouse; 1:100; fig 6	Blanchard J, Eade K, Szucs A, Lo Sardo V, Tsunemoto R, Williams D, et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci. 2015;18:25-35 pubmed publisher
flow cytometry; human; fig s7	Postupna N, Keene C, Latimer C, Sherfield E, Van Gelder R, Ojemann J, et al. Flow cytometry analysis of synaptosomes from post-mortem human brain reveals changes specific to Lewy body and Alzheimer's disease. Lab Invest. 2014;94:1161-72 pubmed publisher
immunohistochemistry; mouse; 1:2000	Li J, Xue Z, Deng S, Luo X, Patrylo P, Rose G, et al. Amyloid plaque pathogenesis in 5XFAD mouse spinal cord: retrograde transneuronal modulation after peripheral nerve injury. Neurotox Res. 2013;24:1-14 pubmed publisher
	Meng X, McGraw C, Wang W, Jing J, Yeh S, Wang L, et al. Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions. elife. 2019;8: pubmed publisher
	Cicvaric A, Yang J, Bulat T, Zambon A, Dominguez Rodriguez M, Kühn R, et al. Enhanced synaptic plasticity and spatial memory in female but not male FLRT2-haplodeficient mice. Sci Rep. 2018;8:3703 pubmed publisher
	Yan L, Li Y, Shi Z, Lu X, Ma J, Hu B, et al. The zinc finger E-box-binding homeobox 1 (Zeb1) promotes the conversion of mouse fibroblasts into functional neurons. J Biol Chem. 2017;292:12959-12970 pubmed publisher
	Zaccaria A, Bouamrani A, ChabardÃ¨s S, El Atifi M, Seigneuret E, Lobrinus J, et al. Deep brain stimulation-associated brain tissue imprints: a new in vivo approach to biological research in human Parkinson's disease. Mol Neurodegener. 2016;11:12 pubmed publisher
	Li J, Cai Y, Liu F, Yang L, Hu X, Patrylo P, et al. Experimental microembolism induces localized neuritic pathology in guinea pig cerebrum. Oncotarget. 2015;6:10772-85 pubmed
	Song C, Feodorova Y, Guy J, Peichl L, Jost K, Kimura H, et al. DNA methylation reader MECP2: cell type- and differentiation stage-specific protein distribution. Epigenetics Chromatin. 2014;7:17 pubmed publisher
	Fazzari P, Snellinx A, Sabanov V, Ahmed T, Serneels L, Gärtner A, et al. Cell autonomous regulation of hippocampal circuitry via Aph1b-?-secretase/neuregulin 1 signalling. elife. 2014;3: pubmed publisher
	Shinoyama M, Ideguchi M, Kida H, Kajiwara K, Kagawa Y, Maeda Y, et al. Cortical region-specific engraftment of embryonic stem cell-derived neural progenitor cells restores axonal sprouting to a subcortical target and achieves motor functional recovery in a mouse model of neonatal hypoxic-ischemic brain injury. Front Cell Neurosci. 2013;7:128 pubmed publisher
	Srivastava D, Copits B, Xie Z, Huda R, Jones K, Mukherji S, et al. Afadin is required for maintenance of dendritic structure and excitatory tone. J Biol Chem. 2012;287:35964-74 pubmed publisher
	Mangin J, Li P, Scafidi J, Gallo V. Experience-dependent regulation of NG2 progenitors in the developing barrel cortex. Nat Neurosci. 2012;15:1192-4 pubmed publisher
	Mandemakers W, Snellinx A, O Neill M, De Strooper B. LRRK2 expression is enriched in the striosomal compartment of mouse striatum. Neurobiol Dis. 2012;48:582-93 pubmed publisher
	Tirko N, Ryugo D. Synaptic plasticity in the medial superior olive of hearing, deaf, and cochlear-implanted cats. J Comp Neurol. 2012;520:2202-17 pubmed publisher
	Cai Y, Zhang X, Macklin L, Cai H, Luo X, Oddo S, et al. BACE1 elevation is involved in amyloid plaque development in the triple transgenic model of Alzheimer's disease: differential AÎ² antibody labeling of early-onset axon terminal pathology. Neurotox Res. 2012;21:160-74 pubmed publisher
	Yamamori S, Itakura M, Sugaya D, Katsumata O, Sakagami H, Takahashi M. Differential expression of SNAP-25 family proteins in the mouse brain. J Comp Neurol. 2011;519:916-32 pubmed publisher
	Cai Y, Xiong K, Zhang X, Cai H, Luo X, Feng J, et al. ?-Secretase-1 elevation in aged monkey and Alzheimer's disease human cerebral cortex occurs around the vasculature in partnership with multisystem axon terminal pathogenesis and ?-amyloid accumulation. Eur J Neurosci. 2010;32:1223-38 pubmed publisher
	Zhang X, Xiong K, Cai Y, Cai H, Luo X, Feng J, et al. Functional deprivation promotes amyloid plaque pathogenesis in Tg2576 mouse olfactory bulb and piriform cortex. Eur J Neurosci. 2010;31:710-21 pubmed publisher
	Neal A, Stansfield K, Worley P, Thompson R, Guilarte T. Lead exposure during synaptogenesis alters vesicular proteins and impairs vesicular release: potential role of NMDA receptor-dependent BDNF signaling. Toxicol Sci. 2010;116:249-63 pubmed publisher
	Zhang X, Cai Y, Xiong K, Cai H, Luo X, Feng J, et al. Beta-secretase-1 elevation in transgenic mouse models of Alzheimer's disease is associated with synaptic/axonal pathology and amyloidogenesis: implications for neuritic plaque development. Eur J Neurosci. 2009;30:2271-83 pubmed publisher
	Keniston L, Henderson S, Meredith M. Neuroanatomical identification of crossmodal auditory inputs to interneurons in somatosensory cortex. Exp Brain Res. 2010;202:725-31 pubmed publisher

product information

Catalog Number :

MAB5502

Subcategory :

Neuroscience

Product Name :

Anti-Vesicular Glutamate Transporter 1 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q62634

Host Name :

Mouse

Antigen :

Vesicular Glutamate Transporter 1

Clone :

3C10.2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Vesicular Glutamate Transporter 1 Antibody

Cross Reactivity :

Rat;Mouse

Background :

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate. Vesicular glutamate transporters pack the neurotransmitter into synaptic vesicles so that they can be released into the synapse. VGLUTs are dependent on a proton gradient that they create by hydrolysing adenosine triphosphate (ATP). VGLUTs have only between one hundredth and one thousandth the affinity for glutamate that EAATs have.

ALT Names :

Solute carrier family 17 member 7;Brain-specific Na(+)-dependent inorganic phosphate cotransporter

Immunogen :

Recombinant protein from rat VGLUT1.

Specificity :

Reacts with VGLUT1 (Vesicular Glutamate Transporter 1).

Package Size :

100 µg

Uses :

Immunohistochemistry;Western Blotting

Storage :

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.