Notch 1 Antibody, extracellular, clone 8G10 | EMD Millipore MAB5414 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Notch 1 Antibody, extracellular, clone 8G10

catalog :

MAB5414

quantity :

100 µL

clonality :

monoclonal

host :

Syrian golden hamster

conjugate :

nonconjugated

clone name :

8G10

The same clone is also sold as:

Santa Cruz Biotechnology: sc-32756

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, flow cytometry

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 5a	Steinbuck M, Arakcheeva K, Winandy S. Novel TCR-Mediated Mechanisms of Notch Activation and Signaling. J Immunol. 2018;200:997-1007 pubmed publisher
flow cytometry; rat	Boopathy A, Che P, Somasuntharam I, Fiore V, Cabigas E, Ban K, et al. The modulation of cardiac progenitor cell function by hydrogel-dependent Notch1 activation. Biomaterials. 2014;35:8103-12 pubmed publisher
	Idogawa M, Ohashi T, Sugisaka J, Sasaki Y, Suzuki H, Tokino T. Array-based genome-wide RNAi screening to identify shRNAs that enhance p53-related apoptosis in human cancer cells. Oncotarget. 2014;5:7540-8 pubmed
	Wang S, Sun W, Zhao Y, McEachern D, Meaux I, BarriÃ¨re C, et al. SAR405838: an optimized inhibitor of MDM2-p53 interaction that induces complete and durable tumor regression. Cancer Res. 2014;74:5855-65 pubmed publisher
	Chou C, Huang N, Jhuang S, Pan H, Peng N, Cheng J, et al. Ubiquitin-conjugating enzyme UBE2C is highly expressed in breast microcalcification lesions. PLoS ONE. 2014;9:e93934 pubmed publisher
	Guerzoni C, Amatori S, Giorgi L, Manara M, Landuzzi L, Lollini P, et al. An aza-macrocycle containing maltolic side-arms (maltonis) as potential drug against human pediatric sarcomas. BMC Cancer. 2014;14:137 pubmed publisher
	Sun F, Mao X, Xie L, Ding M, Shao B, Jin K. Notch1 signaling modulates neuronal progenitor activity in the subventricular zone in response to aging and focal ischemia. Aging Cell. 2013;12:978-87 pubmed publisher
	Huang B, Ray P, Iwasaki K, Tsuji Y. Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. FASEB J. 2013;27:3763-74 pubmed publisher
	Guy C, Vignali K, Temirov J, Bettini M, Overacre A, Smeltzer M, et al. Distinct TCR signaling pathways drive proliferation and cytokine production in T cells. Nat Immunol. 2013;14:262-70 pubmed publisher
	Brizuela L, Ader I, Mazerolles C, Bocquet M, Malavaud B, Cuvillier O. First evidence of sphingosine 1-phosphate lyase protein expression and activity downregulation in human neoplasm: implication for resistance to therapeutics in prostate cancer. Mol Cancer Ther. 2012;11:1841-51 pubmed publisher
	Leopoldino A, Squarize C, Garcia C, Almeida L, Pestana C, Sobral L, et al. SET protein accumulates in HNSCC and contributes to cell survival: antioxidant defense, Akt phosphorylation and AVOs acidification. Oral Oncol. 2012;48:1106-13 pubmed publisher
	Crose L, Etheridge K, Chen C, Belyea B, Talbot L, Bentley R, et al. FGFR4 blockade exerts distinct antitumorigenic effects in human embryonal versus alveolar rhabdomyosarcoma. Clin Cancer Res. 2012;18:3780-90 pubmed publisher
	Hou X, Tashima Y, Stanley P. Galactose differentially modulates lunatic and manic fringe effects on Delta1-induced NOTCH signaling. J Biol Chem. 2012;287:474-83 pubmed publisher
	Radi E, Formichi P, Di Maio G, Battisti C, Federico A. Oxidative stress-induced apoptosis in two patients with Alagille syndrome. J Neurol Sci. 2011;308:49-56 pubmed publisher
	Leonelli M, Martins D, Britto L. TRPV1 receptors modulate retinal development. Int J Dev Neurosci. 2011;29:405-13 pubmed publisher
	Zhou Y, Feng X, Koh D. Activation of cell death mediated by apoptosis-inducing factor due to the absence of poly(ADP-ribose) glycohydrolase. Biochemistry. 2011;50:2850-9 pubmed publisher
	Nichol D, Shawber C, Fitch M, Bambino K, Sharma A, Kitajewski J, et al. Impaired angiogenesis and altered Notch signaling in mice overexpressing endothelial Egfl7. Blood. 2010;116:6133-43 pubmed publisher
	Kim E, Teh S, Yang J, Chow M, Teh H. TNFR2-deficient memory CD8 T cells provide superior protection against tumor cell growth. J Immunol. 2009;183:6051-7 pubmed publisher
	Shawgo M, Shelton S, Robertson J. Caspase-9 activation by the apoptosome is not required for fas-mediated apoptosis in type II Jurkat cells. J Biol Chem. 2009;284:33447-55 pubmed publisher
	Shelton S, Shawgo M, Matthews S, Lu Y, Donnelly A, Szabla K, et al. KU135, a novel novobiocin-derived C-terminal inhibitor of the 90-kDa heat shock protein, exerts potent antiproliferative effects in human leukemic cells. Mol Pharmacol. 2009;76:1314-22 pubmed publisher
	Chen L, Redkar S, Bearss D, Wierda W, Gandhi V. Pim kinase inhibitor, SGI-1776, induces apoptosis in chronic lymphocytic leukemia cells. Blood. 2009;114:4150-7 pubmed publisher
	Clement C, Dandepally S, Williams A, Ibeanu G. A synthetic analog of verbenachalcone potentiates NGF-induced neurite outgrowth and enhances cell survival in neuronal cell models. Neurosci Lett. 2009;459:157-61 pubmed publisher
	Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P. Roles of Pofut1 and O-fucose in mammalian Notch signaling. J Biol Chem. 2008;283:13638-51 pubmed publisher
	Preyer M, Shu C, Wang J. Delayed activation of Bax by DNA damage in embryonic stem cells with knock-in mutations of the Abl nuclear localization signals. Cell Death Differ. 2007;14:1139-48 pubmed

product information

Catalog Number :

MAB5414

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Notch 1 Antibody, extracellular, clone 8G10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P46531

Host Name :

Syrian Hamster

Antigen :

Notch 1

Clone :

8G10

Conjugate :

Culture Supernatant

Isotype :

IgG1

Product Description :

Anti-Notch 1 Antibody, extracellular, clone 8G10

Cross Reactivity :

Mouse;Rat

Background :

Notch 1 is a type 1 membrane protein that serves as a receptor for the membrane bound ligands jagged-1 and -2 and delta-1 to regulate cell fate determination through the implementation of differentiation, proliferation and apoptotic programs. Upon ligand binding, the Notch intracellular domain (NICD) is released by proteolytic processing and subsequently translocates to the nucleus where it forms a transcriptionally active complex with RBP-J kappa to activate genes of the enhancer of split locus. Notch-1 may also be involved in cell specification and/or differentiation and body segment determination in the postimplantation embryo. In the adult animal, Notch-1 is highly expressed in the brain, lung, and thymus with lower expression levels in the spleen, bone-marrow, spinal cord, eyes, mammary gland, liver, intestine, skeletal muscle, kidney and heart.

ALT Names :

Motch A; mT14; p300

Immunogen :

A GST fusion protein comprising amino acids 1299-1492 of mouse Notch 1

Specificity :

Reacts with natural and recombinant Notch1. Recognizes a band migrating at approximately 300 kDa on Western blots of rat and mouse protein extracts. A cross reactive band migrating at approximately 230 kDa is observed in some samples and is believed to correspond to the processed extracellular fragment of Notch 1.

Package Size :

100 µL

Uses :

Flow Cytometry;Western Blotting;Immunohistochemistry

Storage :

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.