GAD67 Antibody, clone 1G10.2, Biotin Conjugate | EMD Millipore MAB5406B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GAD67 Antibody, clone 1G10.2, Biotin Conjugate

catalog :

MAB5406B

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

1G10.2

reactivity :

human, mouse, rat

application :

immunohistochemistry

citations: 22

Reference
Agca S, Houen G, Trier N. Characterization of continuous B-cell epitopes in the N-terminus of glutamate decarboxylase67 using monoclonal antibodies. J Pept Sci. 2014;20:928-34 pubmed publisher
Gaven F, Marin P, Claeysen S. Primary culture of mouse dopaminergic neurons. J Vis Exp. 2014;:e51751 pubmed publisher
Zizioli D, Guarienti M, Tobia C, Gariano G, Borsani G, Bresciani R, et al. Molecular cloning and knockdown of galactocerebrosidase in zebrafish: new insights into the pathogenesis of Krabbe's disease. Biochim Biophys Acta. 2014;1842:665-75 pubmed publisher
Balu D, Takagi S, Puhl M, Benneyworth M, Coyle J. D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain. Cell Mol Neurobiol. 2014;34:419-35 pubmed publisher
Keeve P, Dittmar T, Gassmann G, Grimm W, Niggemann B, Friedmann A. Characterization and analysis of migration patterns of dentospheres derived from periodontal tissue and the palate. J Periodontal Res. 2013;48:276-85 pubmed publisher
Chapman R, Lall V, Maxeiner S, Willecke K, Deuchars J, King A. Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice. Brain Struct Funct. 2013;218:751-65 pubmed publisher
Gribaudo S, Bovetti S, Friard O, Denorme M, Oboti L, Fasolo A, et al. Transitory and activity-dependent expression of neurogranin in olfactory bulb tufted cells during mouse postnatal development. J Comp Neurol. 2012;520:3055-69 pubmed publisher
Ohishi T, Wang L, Akane H, Shiraki A, Goto K, Ikarashi Y, et al. Reversible aberration of neurogenesis affecting late-stage differentiation in the hippocampal dentate gyrus of rat offspring after maternal exposure to manganese chloride. Reprod Toxicol. 2012;34:408-19 pubmed publisher
Saegusa Y, Fujimoto H, Woo G, Ohishi T, Wang L, Mitsumori K, et al. Transient aberration of neuronal development in the hippocampal dentate gyrus after developmental exposure to brominated flame retardants in rats. Arch Toxicol. 2012;86:1431-42 pubmed publisher
Trifonov S, Houtani T, Kase M, Toida K, Maruyama M, Yamashita Y, et al. Lateral regions of the rodent striatum reveal elevated glutamate decarboxylase 1 mRNA expression in medium-sized projection neurons. Eur J Neurosci. 2012;35:711-22 pubmed publisher
Martin J, Brown A, Balkowiec A. Glia determine the course of brain-derived neurotrophic factor-mediated dendritogenesis and provide a soluble inhibitory cue to dendritic growth in the brainstem. Neuroscience. 2012;207:333-46 pubmed publisher
Kadriu B, Guidotti A, Chen Y, Grayson D. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012;520:1951-64 pubmed publisher
Stranahan A, Jiam N, Spiegel A, Gallagher M. Aging reduces total neuron number in the dorsal component of the rodent prefrontal cortex. J Comp Neurol. 2012;520:1318-26 pubmed publisher
Mészár Z, Girard F, Saper C, Celio M. The lateral hypothalamic parvalbumin-immunoreactive (PV1) nucleus in rodents. J Comp Neurol. 2012;520:798-815 pubmed publisher
Nouel D, Burt M, Zhang Y, Harvey L, Boksa P. Prenatal exposure to bacterial endotoxin reduces the number of GAD67- and reelin-immunoreactive neurons in the hippocampus of rat offspring. Eur Neuropsychopharmacol. 2012;22:300-7 pubmed publisher
Willett R, Greene L. Gata2 is required for migration and differentiation of retinorecipient neurons in the superior colliculus. J Neurosci. 2011;31:4444-55 pubmed publisher
Bøttger P, Tracz Z, Heuck A, Nissen P, Romero Ramos M, Lykke Hartmann K. Distribution of Na/K-ATPase alpha 3 isoform, a sodium-potassium P-type pump associated with rapid-onset of dystonia parkinsonism (RDP) in the adult mouse brain. J Comp Neurol. 2011;519:376-404 pubmed publisher
Kay R, Meyer E, Illig K, Brunjes P. Spatial distribution of neural activity in the anterior olfactory nucleus evoked by odor and electrical stimulation. J Comp Neurol. 2011;519:277-89 pubmed publisher
Ohishi T, Wang L, Ogawa B, Fujisawa K, Taniai E, Hayashi H, et al. No effect of sustained systemic growth retardation on the distribution of Reelin-expressing interneurons in the neuron-producing hippocampal dentate gyrus in rats. Reprod Toxicol. 2010;30:591-9 pubmed publisher
He Y, Hsuchou H, Wu X, Kastin A, Khan R, Pistell P, et al. Interleukin-15 receptor is essential to facilitate GABA transmission and hippocampal-dependent memory. J Neurosci. 2010;30:4725-34 pubmed publisher
Jiang Z, Belforte J, Lu Y, Yabe Y, Pickel J, Smith C, et al. eIF2alpha Phosphorylation-dependent translation in CA1 pyramidal cells impairs hippocampal memory consolidation without affecting general translation. J Neurosci. 2010;30:2582-94 pubmed publisher
Toyoshima M, Sakurai K, Shimazaki K, Takeda Y, Nakamoto M, Serizawa S, et al. Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem. J Comp Neurol. 2009;513:349-62 pubmed publisher

product information

Catalog Number :

MAB5406B

Subcategory :

Neuroscience

Product Name :

Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q99259

Host Name :

Mouse

Antigen :

GAD67

Clone :

1G10.2

Conjugate :

Biotin

Isotype :

IgG2a

Product Description :

Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate

Cross Reactivity :

Human;Mouse;Rat

Background :

Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.

ALT Names :

Glutamate decarboxylase 1;67 kDa glutamic acid decarboxylase;GAD-67;Glutamate decarboxylase 67 kDa isoform

Immunogen :

Recombinant GAD67 protein

Specificity :

Reacts with GAD67. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate.

Package Size :

100 µL

Uses :

Immunohistochemistry

Storage :

Maintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt.