GAD67 Antibody, clone 1G10.2 | EMD Millipore MAB5406 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GAD67 Antibody, clone 1G10.2

catalog :

MAB5406

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1G10.2

reactivity :

common tree shrew , black ferret, guinea pig, tree shrew, African green monkey, gerbils, degu, human, mouse, rat, pigs , domestic rabbit, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 419

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; 1:500; loading ...; fig 2e	Voskobiynyk Y, Roth J, Cochran J, Rush T, Carullo N, Mesina J, et al. Alzheimer's disease risk gene BIN1 induces Tau-dependent network hyperexcitability. elife. 2020;9: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:2000; loading ...; fig 4s1a	Zhang X, Dou Y, Yuan L, Li Q, Zhu Y, Wang M, et al. Different neuronal populations mediate inflammatory pain analgesia by exogenous and endogenous opioids. elife. 2020;9: pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 6g	Zhang R, Liakath Ali K, Sudhof T. Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum. elife. 2020;9: pubmed publisher
immunohistochemistry - frozen section; rat; 1:400; loading ...; fig 2b	Mafi A, Hofer L, Russ M, Young J, Mellott J. The Density of Perineuronal Nets Increases With Age in the Inferior Colliculus in the Fischer Brown Norway Rat. Front Aging Neurosci. 2020;12:27 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 2g	Wu Z, Parry M, Hou X, Liu M, Wang H, Cain R, et al. Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 2020;11:1105 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 1b	Pelkey K, Calvigioni D, Fang C, Vargish G, Ekins T, Auville K, et al. Paradoxical network excitation by glutamate release from VGluT3+ GABAergic interneurons. elife. 2020;9: pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 1e	Wu Y, Chen C, Chen M, Qian K, Lv X, Wang H, et al. The anterior insular cortex unilaterally controls feeding in response to aversive visceral stimuli in mice. Nat Commun. 2020;11:640 pubmed publisher
immunohistochemistry - frozen section; human; 1:1000; loading ...; fig s1e	Trevino A, Sinnott Armstrong N, Andersen J, Yoon S, Huber N, Pritchard J, et al. Chromatin accessibility dynamics in a model of human forebrain development. Science. 2020;367: pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig s1, 1, 2, 4, 6	Su J, Charalambakis N, Sabbagh U, Somaiya R, Monavarfeshani A, Guido W, et al. Retinal inputs signal astrocytes to recruit interneurons into visual thalamus. Proc Natl Acad Sci U S A. 2020;117:2671-2682 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 3a	Anstötz M, Maccaferri G. A Toolbox of Criteria for Distinguishing Cajal-Retzius Cells from Other Neuronal Types in the Postnatal Mouse Hippocampus. Eneuro. 2020;7: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 5j	Stefanov A, Novelli E, Strettoi E. Inner retinal preservation in the photoinducible I307N rhodopsin mutant mouse, a model of autosomal dominant retinitis pigmentosa. J Comp Neurol. 2020;528:1502-1522 pubmed publisher
immunohistochemistry; rat; 1:500; loading ...; fig 6e	Kelley L, Verlezza S, Long H, Loka M, Walker C. Increased Hypothalamic Projections to the Lateral Hypothalamus and Responses to Leptin in Rat Neonates From High Fat Fed Mothers. Front Neurosci. 2019;13:1454 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 1a	Wong A, Borst J. Tonotopic and non-auditory organization of the mouse dorsal inferior colliculus revealed by two-photon imaging. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 2c	Orefice L, Mosko J, Morency D, Wells M, Tasnim A, Mozeika S, et al. Targeting Peripheral Somatosensory Neurons to Improve Tactile-Related Phenotypes in ASD Models. Cell. 2019;178:867-886.e24 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 1b	Chowdhury S, Matsubara T, Miyazaki T, Ono D, Fukatsu N, Abe M, et al. GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 9a	Tulloch A, Teo S, Carvajal B, Tessier Lavigne M, Jaworski A. Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse. J Comp Neurol. 2019;527:2948-2972 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2a	Goyer D, Silveira M, George A, Beebe N, Edelbrock R, Malinski P, et al. A novel class of inferior colliculus principal neurons labeled in vasoactive intestinal peptide-Cre mice. elife. 2019;8: pubmed publisher
western blot; human; 1:200; loading ...	Pandya M, Palpagama T, Turner C, Waldvogel H, Faull R, Kwakowsky A. Sex- and age-related changes in GABA signaling components in the human cortex. Biol Sex Differ. 2019;10:5 pubmed publisher
immunohistochemistry; rat; 1:300; loading ...; fig 2g	Yu Q, Liu Y, Zhu Y, Wang Y, Li Q, Yin D. Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain. Brain Struct Funct. 2019;224:1035-1049 pubmed publisher
immunohistochemistry; guinea pig; 1:100; loading ...; fig 2	Mellott J, Beebe N, Schofield B. Bilateral projections to the thalamus from individual neurons in the inferior colliculus. J Comp Neurol. 2019;527:1118-1126 pubmed publisher
immunohistochemistry; rat; 1:200; loading ...; fig 3f	Breton J, Charbit A, Snyder B, Fong P, Dias E, Himmels P, et al. Relative contributions and mapping of ventral tegmental area dopamine and GABA neurons by projection target in the rat. J Comp Neurol. 2019;527:916-941 pubmed publisher
immunohistochemistry; tree shrew; 1:1000; loading ...; fig 6g	Johnson E, Westbrook T, Shayesteh R, Chen E, Schumacher J, Fitzpatrick D, et al. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. J Comp Neurol. 2019;527:328-344 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; tbl 1	Ghinia M, Novelli E, Sajgo S, Badea T, Strettoi E. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. J Comp Neurol. 2019;527:187-211 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:3000; loading ...; fig 7b	Jain S, Glubrecht D, Germain D, Moser M, Godbout R. AP-2Îµ Expression in Developing Retina: Contributing to the Molecular Diversity of Amacrine Cells. Sci Rep. 2018;8:3386 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:800; loading ...; fig 2b	Bayguinov P, Ma Y, Gao Y, Zhao X, Jackson M. Imaging Voltage in Genetically Defined Neuronal Subpopulations with a Cre Recombinase-Targeted Hybrid Voltage Sensor. J Neurosci. 2017;37:9305-9319 pubmed publisher
immunocytochemistry; mouse; 1:1000; fig 1a, 3a. 5a	McCullagh E, Salcedo E, Huntsman M, Klug A. Tonotopic alterations in inhibitory input to the medial nucleus of the trapezoid body in a mouse model of Fragile X syndrome. J Comp Neurol. 2017;525:3543-3562 pubmed publisher
immunocytochemistry; mouse; 1:500; loading ...; fig s1e	Young F, Keruzore M, Nan X, Gennet N, Bellefroid E, Li M. The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1. Proc Natl Acad Sci U S A. 2017;114:E5599-E5607 pubmed publisher
immunohistochemistry; human; 1:1000; fig 1f	Birey F, Andersen J, Makinson C, Islam S, Wei W, Huber N, et al. Assembly of functionally integrated human forebrain spheroids. Nature. 2017;545:54-59 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:2000; loading ...; fig s4a	Castellano J, Mosher K, Abbey R, McBride A, James M, Berdnik D, et al. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature. 2017;544:488-492 pubmed publisher
immunohistochemistry; mouse; 1:700; fig S3b	Fonseca M, Chu S, Hernandez M, Fang M, Modarresi L, Selvan P, et al. Cell-specific deletion of C1qa identifies microglia as the dominant source of C1q in mouse brain. J Neuroinflammation. 2017;14:48 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 4b immunocytochemistry; mouse; 1:1000; loading ...; fig 5-s1a	Ripamonti S, Ambrozkiewicz M, Guzzi F, Gravati M, Biella G, Bormuth I, et al. Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons. elife. 2017;6: pubmed publisher
immunohistochemistry - frozen section; human; loading ...; fig 3h	Sartore R, Cardoso S, Lages Y, Paraguassu J, Stelling M, Madeiro da Costa R, et al. Trace elements during primordial plexiform network formation in human cerebral organoids. Peerj. 2017;5:e2927 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig s4c	Li Y, Andereggen L, Yuki K, Omura K, Yin Y, Gilbert H, et al. Mobile zinc increases rapidly in the retina after optic nerve injury and regulates ganglion cell survival and optic nerve regeneration. Proc Natl Acad Sci U S A. 2017;114:E209-E218 pubmed publisher
immunohistochemistry; rat; 1:500; loading ...; fig 1b	Calakos K, Blackman D, Schulz A, Bauer E. Distribution of type I corticotropin-releasing factor (CRF1) receptors on GABAergic neurons within the basolateral amygdala. Synapse. 2017;71: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5b	Ueno H, Suemitsu S, Okamoto M, Matsumoto Y, Ishihara T. Parvalbumin neurons and perineuronal nets in the mouse prefrontal cortex. Neuroscience. 2017;343:115-127 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig s7a	Justus D, DalÃ¼gge D, Bothe S, Fuhrmann F, Hannes C, Kaneko H, et al. Glutamatergic synaptic integration of locomotion speed via septoentorhinal projections. Nat Neurosci. 2017;20:16-19 pubmed publisher
immunocytochemistry; human; 1:500; loading ...	Deflorio C, Blanchard S, Carisì M, Bohl D, Maskos U. Human polymorphisms in nicotinic receptors: a functional analysis in iPS-derived dopaminergic neurons. FASEB J. 2017;31:828-839 pubmed publisher
immunocytochemistry; gerbils; 1:1000; loading ...; fig 4b immunocytochemistry; black ferret; 1:1000; loading ...; fig 4c	Dimidschstein J, Chen Q, Tremblay R, Rogers S, Saldi G, Guo L, et al. A viral strategy for targeting and manipulating interneurons across vertebrate species. Nat Neurosci. 2016;19:1743-1749 pubmed publisher
immunohistochemistry; mouse; 1:250; fig 2c	Fujimura K, Mitsuhashi T, Shibata S, Shimozato S, Takahashi T. In Utero Exposure to Valproic Acid Induces Neocortical Dysgenesis via Dysregulation of Neural Progenitor Cell Proliferation/Differentiation. J Neurosci. 2016;36:10908-10919 pubmed
immunohistochemistry; rat; 1:100; loading ...; fig 4a	Villanueva Castillo C, Tecuatl C, Herrera LÃ³pez G, GalvÃ¡n E. Aging-related impairments of hippocampal mossy fibers synapses on CA3 pyramidal cells. Neurobiol Aging. 2017;49:119-137 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:800; loading ...; fig 1c	Lin C, Huang S, Tung C, Chou C, Gau S, Huang H. Analysis of Genome-Wide Monoallelic Expression Patterns in Three Major Cell Types of Mouse Visual Cortex Using Laser Capture Microdissection. PLoS ONE. 2016;11:e0163663 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; tbl 1	Patel M, Sons S, Yudintsev G, Lesicko A, Yang L, Taha G, et al. Anatomical characterization of subcortical descending projections to the inferior colliculus in mouse. J Comp Neurol. 2017;525:885-900 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 5	Fukada M, Nakayama A, Mamiya T, Yao T, Kawaguchi Y. Dopaminergic abnormalities in Hdac6-deficient mice. Neuropharmacology. 2016;110:470-479 pubmed publisher
immunohistochemistry; mouse; 1:500; fig 3	Inoue Y, Inoue T. Brain enhancer activities at the gene-poor 5p14.1 autism-associated locus. Sci Rep. 2016;6:31227 pubmed publisher
immunocytochemistry; human; 1:1000; fig 3b	Sun Y, Paşca S, Portmann T, Goold C, Worringer K, Guan W, et al. A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients. elife. 2016;5: pubmed publisher
immunohistochemistry; mouse; loading ...; fig st1	Ku T, Swaney J, Park J, Albanese A, Murray E, Cho J, et al. Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nat Biotechnol. 2016;34:973-81 pubmed publisher
immunohistochemistry; mouse; 1:800; fig 3	Senzacqua M, Severi I, Perugini J, Acciarini S, Cinti S, Giordano A. Action of Administered Ciliary Neurotrophic Factor on the Mouse Dorsal Vagal Complex. Front Neurosci. 2016;10:289 pubmed publisher
immunohistochemistry; human; 1:500; fig 1	Gotts J, Atkinson L, Yanagawa Y, Deuchars J, Deuchars S. Co-expression of GAD67 and choline acetyltransferase in neurons in the mouse spinal cord: A focus on lamina X. Brain Res. 2016;1646:570-579 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1000; loading ...; fig 7a western blot; rat; fig 8b	Botterill J, Nogovitsyn N, Caruncho H, Kalynchuk L. Selective plasticity of hippocampal GABAergic interneuron populations following kindling of different brain regions. J Comp Neurol. 2017;525:389-406 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 1	Ure K, Lu H, Wang W, Ito Ishida A, Wu Z, He L, et al. Restoration of Mecp2 expression in GABAergic neurons is sufficient to rescue multiple disease features in a mouse model of Rett syndrome. elife. 2016;5: pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500; fig s1i	Figueres Oñate M, López Mascaraque L. Adult Olfactory Bulb Interneuron Phenotypes Identified by Targeting Embryonic and Postnatal Neural Progenitors. Front Neurosci. 2016;10:194 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; fig 1	Reinhard J, Kriz A, Galic M, Angliker N, Rajalu M, Vogt K, et al. The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Nat Commun. 2016;7:11613 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 5f	Renda A, Penty N, Komal P, Nashmi R. Vulnerability to nicotine self-administration in adolescent mice correlates with age-specific expression of ?4* nicotinic receptors. Neuropharmacology. 2016;108:49-59 pubmed publisher
immunohistochemistry; rat; 1:2500; fig S1	Wang X, Song X, Wu L, Nadler J, Zhan R. Persistent Hyperactivity of Hippocampal Dentate Interneurons After a Silent Period in the Rat Pilocarpine Model of Epilepsy. Front Cell Neurosci. 2016;10:94 pubmed publisher
immunohistochemistry - free floating section; rat; 1:2000; fig 7	Zhang L, Hernandez V, Vázquez Juárez E, Chay F, Barrio R. Thirst Is Associated with Suppression of Habenula Output and Active Stress Coping: Is there a Role for a Non-canonical Vasopressin-Glutamate Pathway?. Front Neural Circuits. 2016;10:13 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig s8	Wells M, Wimmer R, Schmitt L, Feng G, Halassa M. Thalamic reticular impairment underlies attention deficit in Ptchd1(Y/-) mice. Nature. 2016;532:58-63 pubmed publisher
immunohistochemistry; rat; loading ...; fig 5c	Um J, Choii G, Park D, Kim D, Jeon S, Kang H, et al. IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation. J Biol Chem. 2016;291:10119-30 pubmed publisher
immunohistochemistry; mouse; 1:500; fig 1	Monai H, Ohkura M, Tanaka M, Oe Y, Konno A, Hirai H, et al. Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain. Nat Commun. 2016;7:11100 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 2a	Wasserman D, Tan J, Kim J, Yeomans J. Muscarinic control of rostromedial tegmental nucleus GABA neurons and morphine-induced locomotion. Eur J Neurosci. 2016;44:1761-70 pubmed publisher
western blot; human; 1:1000; fig 4	González Burguera I, Ricobaraza A, Aretxabala X, Barrondo S, Garcia del Caño G, López de Jesús M, et al. Highly efficient generation of glutamatergic/cholinergic NT2-derived postmitotic human neurons by short-term treatment with the nucleoside analogue cytosine ?-D-arabinofuranoside. Stem Cell Res. 2016;16:541-51 pubmed publisher
immunohistochemistry; common tree shrew ; 1:1000; loading ...; fig 3e	Day Brown J, Slusarczyk A, Zhou N, Quiggins R, Petry H, Bickford M. Synaptic organization of striate cortex projections in the tree shrew: A comparison of the claustrum and dorsal thalamus. J Comp Neurol. 2017;525:1403-1420 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4a	Bonini S, Mastinu A, Maccarinelli G, Mitola S, Premoli M, La Rosa L, et al. Cortical Structure Alterations and Social Behavior Impairment in p50-Deficient Mice. Cereb Cortex. 2016;26:2832-49 pubmed publisher
immunohistochemistry; mouse; 1:500; fig 3	Canetta S, Bolkan S, Padilla Coreano N, Song L, Sahn R, Harrison N, et al. Maternal immune activation leads to selective functional deficits in offspring parvalbumin interneurons. Mol Psychiatry. 2016;21:956-68 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 6	Buzhdygan T, Lisinicchia J, Patel V, Johnson K, Neugebauer V, Paessler S, et al. Neuropsychological, Neurovirological and Neuroimmune Aspects of Abnormal GABAergic Transmission in HIV Infection. J Neuroimmune Pharmacol. 2016;11:279-93 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig s3a	Deverman B, Pravdo P, Simpson B, Kumar S, Chan K, Banerjee A, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nat Biotechnol. 2016;34:204-9 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1000 western blot; rat; 1:5000	Lee Kubli C, Ingves M, Henry K, Shiao R, Collyer E, Tuszynski M, et al. Analysis of the behavioral, cellular and molecular characteristics of pain in severe rodent spinal cord injury. Exp Neurol. 2016;278:91-104 pubmed publisher
immunocytochemistry; human; 1:500; tbl 1	de Souza C, Nivison Smith L, Christie D, Polkinghorne P, McGhee C, Kalloniatis M, et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp Eye Res. 2016;150:135-48 pubmed publisher
immunohistochemistry; mouse; 1:20,000; loading ...	Wagener R, Witte M, Guy J, Mingo Moreno N, KÃ¼gler S, Staiger J. Thalamocortical Connections Drive Intracortical Activation of Functional Columns in the Mislaminated Reeler Somatosensory Cortex. Cereb Cortex. 2016;26:820-37 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:10,000; fig 3	Cook Snyder D, Jones A, Reijmers L. A retrograde adeno-associated virus for collecting ribosome-bound mRNA from anatomically defined projection neurons. Front Mol Neurosci. 2015;8:56 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 8	Giordano C, Vinet J, Curia G, Biagini G. Repeated 6-Hz Corneal Stimulation Progressively Increases FosB/Î”FosB Levels in the Lateral Amygdala and Induces Seizure Generalization to the Hippocampus. PLoS ONE. 2015;10:e0141221 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1000; fig 7	Rajbhandari A, Baldo B, Bakshi V. Predator Stress-Induced CRF Release Causes Enduring Sensitization of Basolateral Amygdala Norepinephrine Systems that Promote PTSD-Like Startle Abnormalities. J Neurosci. 2015;35:14270-85 pubmed publisher
immunocytochemistry; mouse; 1:500; tbl 1	Hirata H, Umemori J, Yoshioka H, Koide T, Watanabe K, Shimoda Y. Cell adhesion molecule contactin-associated protein 3 is expressed in the mouse basal ganglia during early postnatal stages. J Neurosci Res. 2016;94:74-89 pubmed publisher
immunocytochemistry; mouse; 1:1000; fig 4	Ehrich J, Messinger D, Knakal C, Kuhar J, Schattauer S, Bruchas M, et al. Kappa Opioid Receptor-Induced Aversion Requires p38 MAPK Activation in VTA Dopamine Neurons. J Neurosci. 2015;35:12917-31 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:5000; fig s1	Wood J, Verma D, Lach G, Bonaventure P, Herzog H, Sperk G, et al. Structure and function of the amygdaloid NPY system: NPY Y2 receptors regulate excitatory and inhibitory synaptic transmission in the centromedial amygdala. Brain Struct Funct. 2016;221:3373-91 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:1000; loading ...; fig 6-1b	Hwang H, Zhang E, Park S, Chung W, Lee S, Kim D, et al. TWIK-Related Spinal Cord Kâº Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation. Yonsei Med J. 2015;56:1307-15 pubmed publisher
immunohistochemistry; rat; 1:10000	Kaufling J, Aston Jones G. Persistent Adaptations in Afferents to Ventral Tegmental Dopamine Neurons after Opiate Withdrawal. J Neurosci. 2015;35:10290-303 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 2	Hooper A, Maguire J. Characterization of a novel subtype of hippocampal interneurons that express corticotropin-releasing hormone. Hippocampus. 2016;26:41-53 pubmed publisher
immunocytochemistry; mouse; 1:200	Liu Y, Miao Q, Yuan J, Han S, Zhang P, Li S, et al. Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo. J Neurosci. 2015;35:9336-55 pubmed publisher
western blot; mouse	Choi J, Kim J, Kim T, Park J, Lee J, Kim H, et al. TRH and TRH receptor system in the basolateral amygdala mediate stress-induced depression-like behaviors. Neuropharmacology. 2015;97:346-56 pubmed publisher
immunohistochemistry; mouse; 1:1000	Jin K, Jiang H, Xiao D, Zou M, Zhu J, Xiang M. Tfap2a and 2b act downstream of Ptf1a to promote amacrine cell differentiation during retinogenesis. Mol Brain. 2015;8:28 pubmed publisher
immunohistochemistry; mouse; 1:200; fig 3	Krakowiak J, Liu C, Papudesu C, Ward P, Wilhelm J, English A. Neuronal BDNF signaling is necessary for the effects of treadmill exercise on synaptic stripping of axotomized motoneurons. Neural Plast. 2015;2015:392591 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s4a	Wei P, Liu N, Zhang Z, Liu X, Tang Y, He X, et al. Processing of visually evoked innate fear by a non-canonical thalamic pathway. Nat Commun. 2015;6:6756 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:1000	Gouty Colomer L, Hosseini B, Marcelo I, Schreiber J, Slump D, Yamaguchi S, et al. Arc expression identifies the lateral amygdala fear memory trace. Mol Psychiatry. 2016;21:364-75 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1000	Javdani F, HollÃ³ K, HegedÅ±s K, Kis G, Hegyi Z, DÃ³cs K, et al. Differential expression patterns of K(+) /Cl(-) cotransporter 2 in neurons within the superficial spinal dorsal horn of rats. J Comp Neurol. 2015;523:1967-83 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 4E immunohistochemistry; rat; 1:1000; fig 4B	PÃ©rez de Sevilla MÃ¼ller L, Sargoy A, FernÃ¡ndez SÃ¡nchez L, Rodriguez A, Liu J, Cuenca N, et al. Expression and cellular localization of the voltage-gated calcium channel Î±2Î´3 in the rodent retina. J Comp Neurol. 2015;523:1443-60 pubmed publisher
immunohistochemistry; rat; 1:1000; fig 2	Seeger Armbruster S, Bosch Bouju C, Little S, Smither R, Hughes S, Hyland B, et al. Patterned, but not tonic, optogenetic stimulation in motor thalamus improves reaching in acute drug-induced Parkinsonian rats. J Neurosci. 2015;35:1211-6 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:300	Madrigal M, Moreno Bravo J, MartÃnez LÃ³pez J, MartÃnez S, Puelles E. Mesencephalic origin of the rostral Substantia nigra pars reticulata. Brain Struct Funct. 2016;221:1403-12 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000	Puighermanal E, Biever A, Espallergues J, Gangarossa G, De Bundel D, Valjent E. drd2-cre:ribotag mouse line unravels the possible diversity of dopamine d2 receptor-expressing cells of the dorsal mouse hippocampus. Hippocampus. 2015;25:858-75 pubmed publisher
immunohistochemistry - free floating section; rat; 1:500; loading ...; fig 3e	Deidda G, Allegra M, Cerri C, Naskar S, Bony G, Zunino G, et al. Early depolarizing GABA controls critical-period plasticity in the rat visual cortex. Nat Neurosci. 2015;18:87-96 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500	Sharaf A, Rahhal B, Spittau B, Roussa E. Localization of reelin signaling pathway components in murine midbrain and striatum. Cell Tissue Res. 2015;359:393-407 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig s6b	Lee Y, Ehninger D, Zhou M, Oh J, Kang M, Kwak C, et al. Mechanism and treatment for learning and memory deficits in mouse models of Noonan syndrome. Nat Neurosci. 2014;17:1736-43 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500	Levy C, Brooks J, Chen J, Su J, Fox M. Cell-specific and developmental expression of lectican-cleaving proteases in mouse hippocampus and neocortex. J Comp Neurol. 2015;523:629-48 pubmed publisher
immunohistochemistry - free floating section; rat; 1:500	SÃ¡nchez PÃ©rez A, Arnal Vicente I, Santos F, Pereira C, ElMlili N, Sanjuan J, et al. Septal projections to nucleus incertus in the rat: bidirectional pathways for modulation of hippocampal function. J Comp Neurol. 2015;523:565-88 pubmed publisher
immunocytochemistry; rat; 1:1000	Masuda C, Takeuchi S, J Bisem N, R Vincent S, Tooyama I. Immunohistochemical Localization of an Isoform of TRK-Fused Gene-Like Protein in the Rat Retina. Acta Histochem Cytochem. 2014;47:75-83 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:750; loading ...; fig 4b	Calfa G, Li W, Rutherford J, Pozzo Miller L. Excitation/inhibition imbalance and impaired synaptic inhibition in hippocampal area CA3 of Mecp2 knockout mice. Hippocampus. 2015;25:159-68 pubmed publisher
immunocytochemistry; mouse; 1:1000	Liu C, Bickford L, Held R, Nyitrai H, SÃ¼dhof T, Kaeser P. The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons. J Neurosci. 2014;34:12289-303 pubmed publisher
immunohistochemistry; mouse; 1:100	Wang H, Yuan Y, Zhang Z, Yan H, Feng Y, Li W. Dysbindin-1C is required for the survival of hilar mossy cells and the maturation of adult newborn neurons in dentate gyrus. J Biol Chem. 2014;289:29060-72 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1000	Apland J, Aroniadou Anderjaska V, Figueiredo T, Rossetti F, Miller S, Braga M. The limitations of diazepam as a treatment for nerve agent-induced seizures and neuropathology in rats: comparison with UBP302. J Pharmacol Exp Ther. 2014;351:359-72 pubmed publisher
immunohistochemistry - free floating section; mouse	Albrecht O, Dondzillo A, Mayer F, Thompson J, Klug A. Inhibitory projections from the ventral nucleus of the trapezoid body to the medial nucleus of the trapezoid body in the mouse. Front Neural Circuits. 2014;8:83 pubmed publisher
immunohistochemistry - free floating section; rat; 1:500	Iceman K, Corcoran A, Taylor B, Harris M. CO2-inhibited neurons in the medullary raphé are GABAergic. Respir Physiol Neurobiol. 2014;203:28-34 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1000	Almeida Suhett C, Prager E, Pidoplichko V, Figueiredo T, Marini A, Li Z, et al. Reduced GABAergic inhibition in the basolateral amygdala and the development of anxiety-like behaviors after mild traumatic brain injury. PLoS ONE. 2014;9:e102627 pubmed publisher
immunohistochemistry; mouse; 1:100	Renda A, Nashmi R. Chronic nicotine pretreatment is sufficient to upregulate ?4* nicotinic receptors and increase oral nicotine self-administration in mice. BMC Neurosci. 2014;15:89 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:800	Flavin S, Matthews R, Wang Q, Muly E, Winder D. ?(2A)-adrenergic receptors filter parabrachial inputs to the bed nucleus of the stria terminalis. J Neurosci. 2014;34:9319-31 pubmed publisher
immunohistochemistry; rat; 1:1000	Gaillard F, Kuny S, Sauve Y. Retinal distribution of Disabled-1 in a diurnal murine rodent, the Nile grass rat Arvicanthis niloticus. Exp Eye Res. 2014;125:236-43 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Shivers K, Nikolopoulou A, Machlovi S, Vallabhajosula S, Figueiredo Pereira M. PACAP27 prevents Parkinson-like neuronal loss and motor deficits but not microglia activation induced by prostaglandin J2. Biochim Biophys Acta. 2014;1842:1707-19 pubmed publisher
immunohistochemistry; mouse; 1:200	Liu C, Ward P, English A. The effects of exercise on synaptic stripping require androgen receptor signaling. PLoS ONE. 2014;9:e98633 pubmed publisher
immunocytochemistry; mouse; 1:500	Huang H, Yoon B, Brooks S, Bakal R, Berrios J, Larsen R, et al. Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice. PLoS ONE. 2014;9:e98383 pubmed publisher
immunohistochemistry; rat; 1:1000	Ito T, Oliver D. Local and commissural IC neurons make axosomatic inputs on large GABAergic tectothalamic neurons. J Comp Neurol. 2014;522:3539-54 pubmed publisher
western blot; domestic rabbit	Vose L, Vinukonda G, Diamond D, Korumilli R, Hu F, Zia M, et al. Prenatal betamethasone does not affect glutamatergic or GABAergic neurogenesis in preterm newborns. Neuroscience. 2014;270:148-57 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Deng J, Wan Y, Wang X, Cohen S, Wetsel W, Greenberg M, et al. MeCP2 phosphorylation limits psychostimulant-induced behavioral and neuronal plasticity. J Neurosci. 2014;34:4519-27 pubmed publisher
immunohistochemistry; mouse; 1:1000	Mishra A, Traut M, Becker L, Klopstock T, Stein V, Klein R. Genetic evidence for the adhesion protein IgSF9/Dasm1 to regulate inhibitory synapse development independent of its intracellular domain. J Neurosci. 2014;34:4187-99 pubmed publisher
immunocytochemistry; mouse; 1:200	Cheng L, Hu W, Qiu B, Zhao J, Yu Y, Guan W, et al. Generation of neural progenitor cells by chemical cocktails and hypoxia. Cell Res. 2014;24:665-79 pubmed publisher
immunohistochemistry; mouse; fig 2	West A, Cowell R, Daher J, Moehle M, Hinkle K, Melrose H, et al. Differential LRRK2 expression in the cortex, striatum, and substantia nigra in transgenic and nontransgenic rodents. J Comp Neurol. 2014;522:2465-80 pubmed publisher
immunohistochemistry; mouse; 1:2000	Uchida T, Furukawa T, Iwata S, Yanagawa Y, Fukuda A. Selective loss of parvalbumin-positive GABAergic interneurons in the cerebral cortex of maternally stressed Gad1-heterozygous mouse offspring. Transl Psychiatry. 2014;4:e371 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000	Ziemli ska E, K gler S, Schachner M, Wewi r I, Czarkowska Bauch J, Skup M. Overexpression of BDNF increases excitability of the lumbar spinal network and leads to robust early locomotor recovery in completely spinalized rats. PLoS ONE. 2014;9:e88833 pubmed publisher
western blot; human; 1:5000	Ippolito J, Piwnica Worms D. A fluorescence-coupled assay for gamma aminobutyric acid (GABA) reveals metabolic stress-induced modulation of GABA content in neuroendocrine cancer. PLoS ONE. 2014;9:e88667 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1000	Prager E, Aroniadou Anderjaska V, Almeida Suhett C, Figueiredo T, Apland J, Rossetti F, et al. The recovery of acetylcholinesterase activity and the progression of neuropathological and pathophysiological alterations in the rat basolateral amygdala after soman-induced status epilepticus: relation to anxiety-like behavior. Neuropharmacology. 2014;81:64-74 pubmed publisher
immunocytochemistry; mouse; 1:500	Balu D, Takagi S, Puhl M, Benneyworth M, Coyle J. D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain. Cell Mol Neurobiol. 2014;34:419-35 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:1000	Ikrar T, Guo N, He K, Besnard A, Levinson S, Hill A, et al. Adult neurogenesis modifies excitability of the dentate gyrus. Front Neural Circuits. 2013;7:204 pubmed publisher
immunocytochemistry; mouse; 1:500	Seo S, Leitch B. Altered thalamic GABAA-receptor subunit expression in the stargazer mouse model of absence epilepsy. Epilepsia. 2014;55:224-32 pubmed publisher
immunohistochemistry; mouse; 1:2000	Holmstrand E, Lund D, Cherian A, Wright J, Martin R, Ennis E, et al. Transgenic overexpression of the presynaptic choline transporter elevates acetylcholine levels and augments motor endurance. Neurochem Int. 2014;73:217-28 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000 western blot; mouse; 1:5000	Beitnere U, van Groen T, Kumar A, Jansone B, Klusa V, Kadish I. Mildronate improves cognition and reduces amyloid-? pathology in transgenic Alzheimer's disease mice. J Neurosci Res. 2014;92:338-46 pubmed publisher
immunohistochemistry - frozen section; pigs ; 1:25	Nakamoto K, Sowick C, Schofield B. Auditory cortical axons contact commissural cells throughout the guinea pig inferior colliculus. Hear Res. 2013;306:131-44 pubmed publisher
immunocytochemistry; mouse; 1:2000	Trotter J, Lee G, Kazdoba T, Crowell B, Domogauer J, Mahoney H, et al. Dab1 is required for synaptic plasticity and associative learning. J Neurosci. 2013;33:15652-68 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1,000	Spiegel A, Koh M, Vogt N, Rapp P, Gallagher M. Hilar interneuron vulnerability distinguishes aged rats with memory impairment. J Comp Neurol. 2013;521:3508-23 pubmed publisher
immunocytochemistry; mouse; 1:1000	Hazra A, Gu F, Aulakh A, Berridge C, Eriksen J, Ziburkus J. Inhibitory neuron and hippocampal circuit dysfunction in an aged mouse model of Alzheimer's disease. PLoS ONE. 2013;8:e64318 pubmed publisher
immunohistochemistry; human; 1:2000	Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, et al. A human Dravet syndrome model from patient induced pluripotent stem cells. Mol Brain. 2013;6:19 pubmed publisher
immunocytochemistry; guinea pig; 1:250	Bonifazi P, Difato F, Massobrio P, Breschi G, Pasquale V, Lévi T, et al. In vitro large-scale experimental and theoretical studies for the realization of bi-directional brain-prostheses. Front Neural Circuits. 2013;7:40 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500	Tochitani S, Kondo S. Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain. PLoS ONE. 2013;8:e56901 pubmed publisher
immunohistochemistry - frozen section; mouse	Takao K, Kobayashi K, Hagihara H, Ohira K, Shoji H, Hattori S, et al. Deficiency of schnurri-2, an MHC enhancer binding protein, induces mild chronic inflammation in the brain and confers molecular, neuronal, and behavioral phenotypes related to schizophrenia. Neuropsychopharmacology. 2013;38:1409-25 pubmed publisher
immunocytochemistry; mouse; 1:1000	Ohtsuka N, Badurek S, Busslinger M, Benes F, Minichiello L, Rudolph U. GABAergic neurons regulate lateral ventricular development via transcription factor Pax5. Genesis. 2013;51:234-45 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:2000; fig 7	Amschl D, Neddens J, Havas D, Flunkert S, Rabl R, Römer H, et al. Time course and progression of wild type ?-synuclein accumulation in a transgenic mouse model. BMC Neurosci. 2013;14:6 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:1,000 immunohistochemistry - paraffin section; mouse; 1:1,000	de Sevilla Müller L, Liu J, Solomon A, Rodriguez A, Brecha N. Expression of voltage-gated calcium channel ?(2)?(4) subunits in the mouse and rat retina. J Comp Neurol. 2013;521:2486-501 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000	Calu D, Kawa A, Marchant N, Navarre B, Henderson M, Chen B, et al. Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats. J Neurosci. 2013;33:214-26 pubmed publisher
immunohistochemistry; mouse; 1:500	Dougherty S, Reeves J, Lesort M, Detloff P, Cowell R. Purkinje cell dysfunction and loss in a knock-in mouse model of Huntington disease. Exp Neurol. 2013;240:96-102 pubmed publisher
immunocytochemistry; mouse; 1:500 western blot; mouse	Heldt S, Mou L, Ressler K. In vivo knockdown of GAD67 in the amygdala disrupts fear extinction and the anxiolytic-like effect of diazepam in mice. Transl Psychiatry. 2012;2:e181 pubmed publisher
immunohistochemistry - frozen section; rat; 1:2000	Shikanai H, Yoshida T, Konno K, Yamasaki M, Izumi T, Ohmura Y, et al. Distinct neurochemical and functional properties of GAD67-containing 5-HT neurons in the rat dorsal raphe nucleus. J Neurosci. 2012;32:14415-26 pubmed publisher
immunohistochemistry; mouse; 1:500	Hutchinson A, Deng J, Cohen S, West A. Phosphorylation of MeCP2 at Ser421 contributes to chronic antidepressant action. J Neurosci. 2012;32:14355-63 pubmed publisher
immunohistochemistry; mouse; 1:4000	Li J, Xue Z, Deng S, Luo X, Patrylo P, Rose G, et al. Amyloid plaque pathogenesis in 5XFAD mouse spinal cord: retrograde transneuronal modulation after peripheral nerve injury. Neurotox Res. 2013;24:1-14 pubmed publisher
immunohistochemistry - free floating section; rat; 2 ug/ml	Nakamura K, Sharott A, Magill P. Temporal coupling with cortex distinguishes spontaneous neuronal activities in identified basal ganglia-recipient and cerebellar-recipient zones of the motor thalamus. Cereb Cortex. 2014;24:81-97 pubmed publisher
immunohistochemistry; mouse; 1:1000	Geis H, Borst J. Large GABAergic neurons form a distinct subclass within the mouse dorsal cortex of the inferior colliculus with respect to intrinsic properties, synaptic inputs, sound responses, and projections. J Comp Neurol. 2013;521:189-202 pubmed publisher
immunohistochemistry; mouse; 1:1000	Chapman R, Lall V, Maxeiner S, Willecke K, Deuchars J, King A. Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice. Brain Struct Funct. 2013;218:751-65 pubmed publisher
immunohistochemistry; mouse; 1:500	Kadriu B, Guidotti A, Chen Y, Grayson D. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012;520:1951-64 pubmed publisher
immunohistochemistry - frozen section; rat; 1:300 or 1:150	Olucha Bordonau F, Otero García M, Sánchez Pérez A, Nunez A, Ma S, Gundlach A. Distribution and targets of the relaxin-3 innervation of the septal area in the rat. J Comp Neurol. 2012;520:1903-39 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500	Wang X, Sun Q. Characterization of axo-axonic synapses in the piriform cortex of Mus musculus. J Comp Neurol. 2012;520:832-47 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000	Stranahan A, Jiam N, Spiegel A, Gallagher M. Aging reduces total neuron number in the dorsal component of the rodent prefrontal cortex. J Comp Neurol. 2012;520:1318-26 pubmed publisher
immunohistochemistry; rat; 1:500	Corteen N, Cole T, Sarna A, Sieghart W, Swinny J. Localization of GABA-A receptor alpha subunits on neurochemically distinct cell types in the rat locus coeruleus. Eur J Neurosci. 2011;34:250-62 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500	Brunjes P, Kay R, Arrivillaga J. The mouse olfactory peduncle. J Comp Neurol. 2011;519:2870-86 pubmed publisher
immunohistochemistry; mouse	Chatzi C, Brade T, Duester G. Retinoic acid functions as a key GABAergic differentiation signal in the basal ganglia. PLoS Biol. 2011;9:e1000609 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:20000 western blot; mouse; 1:1000; fig 4	Stanic D, Mulder J, Watanabe M, Hokfelt T. Characterization of NPY Y2 receptor protein expression in the mouse brain. II. Coexistence with NPY, the Y1 receptor, and other neurotransmitter-related molecules. J Comp Neurol. 2011;519:1219-57 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:1000	Bøttger P, Tracz Z, Heuck A, Nissen P, Romero Ramos M, Lykke Hartmann K. Distribution of Na/K-ATPase alpha 3 isoform, a sodium-potassium P-type pump associated with rapid-onset of dystonia parkinsonism (RDP) in the adult mouse brain. J Comp Neurol. 2011;519:376-404 pubmed publisher
immunohistochemistry; rat; 1:10000	Kay R, Meyer E, Illig K, Brunjes P. Spatial distribution of neural activity in the anterior olfactory nucleus evoked by odor and electrical stimulation. J Comp Neurol. 2011;519:277-89 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:10000	Fu W, Le Maitre E, Fabre V, Bernard J, David Xu Z, Hokfelt T. Chemical neuroanatomy of the dorsal raphe nucleus and adjacent structures of the mouse brain. J Comp Neurol. 2010;518:3464-94 pubmed publisher
immunohistochemistry - free floating section; pigs	Guo C, Hirano A, Stella S, Bitzer M, Brecha N. Guinea pig horizontal cells express GABA, the GABA-synthesizing enzyme GAD 65, and the GABA vesicular transporter. J Comp Neurol. 2010;518:1647-69 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:800	Biancardi V, Campos R, Stern J. Altered balance of gamma-aminobutyric acidergic and glutamatergic afferent inputs in rostral ventrolateral medulla-projecting neurons in the paraventricular nucleus of the hypothalamus of renovascular hypertensive rats. J Comp Neurol. 2010;518:567-85 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Su J, Gorse K, Ramirez F, Fox M. Collagen XIX is expressed by interneurons and contributes to the formation of hippocampal synapses. J Comp Neurol. 2010;518:229-53 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Kotani T, Murata Y, Ohnishi H, Mori M, Kusakari S, Saito Y, et al. Expression of PTPRO in the interneurons of adult mouse olfactory bulb. J Comp Neurol. 2010;518:119-36 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:5000	Gribaudo S, Bovetti S, Garzotto D, Fasolo A, De Marchis S. Expression and localization of the calmodulin-binding protein neurogranin in the adult mouse olfactory bulb. J Comp Neurol. 2009;517:683-94 pubmed publisher
immunohistochemistry - paraffin section; degu; 1:1,000	Delgado L, Vielma A, Kahne T, Palacios A, Schmachtenberg O. The GABAergic system in the retina of neonate and adult Octodon degus, studied by immunohistochemistry and electroretinography. J Comp Neurol. 2009;514:459-72 pubmed publisher
immunohistochemistry; rat; 1:10000	Kaufling J, Veinante P, Pawlowski S, Freund Mercier M, Barrot M. Afferents to the GABAergic tail of the ventral tegmental area in the rat. J Comp Neurol. 2009;513:597-621 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000	Toyoshima M, Sakurai K, Shimazaki K, Takeda Y, Nakamoto M, Serizawa S, et al. Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem. J Comp Neurol. 2009;513:349-62 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:2,000	Muzzi P, Camera P, Di Cunto F, Vercelli A. Deletion of the citron kinase gene selectively affects the number and distribution of interneurons in barrelfield cortex. J Comp Neurol. 2009;513:249-64 pubmed publisher
immunohistochemistry - frozen section; rat; 1:500	Brischoux F, Mainville L, Jones B. Muscarinic-2 and orexin-2 receptors on GABAergic and other neurons in the rat mesopontine tegmentum and their potential role in sleep-wake state control. J Comp Neurol. 2008;510:607-30 pubmed publisher
immunocytochemistry; common tree shrew ; 1:2,000	Chomsung R, Petry H, Bickford M. Ultrastructural examination of diffuse and specific tectopulvinar projections in the tree shrew. J Comp Neurol. 2008;510:24-46 pubmed publisher
immunohistochemistry; African green monkey; 1:400	Tomioka R, Rockland K. Long-distance corticocortical GABAergic neurons in the adult monkey white and gray matter. J Comp Neurol. 2007;505:526-38 pubmed
immunohistochemistry - free floating section; rat; 1:1,000 or 1:3,000	Ito T, Hioki H, Nakamura K, Tanaka Y, Nakade H, Kaneko T, et al. Gamma-aminobutyric acid-containing sympathetic preganglionic neurons in rat thoracic spinal cord send their axons to the superior cervical ganglion. J Comp Neurol. 2007;502:113-25 pubmed
immunohistochemistry - free floating section; mouse; 1:1000	Parrish Aungst S, Shipley M, Erdelyi F, Szabo G, Puche A. Quantitative analysis of neuronal diversity in the mouse olfactory bulb. J Comp Neurol. 2007;501:825-36 pubmed
immunohistochemistry; rat; 2 ug/ml	Kuramoto E, Fujiyama F, Unzai T, Nakamura K, Hioki H, Furuta T, et al. Metabotropic glutamate receptor 4-immunopositive terminals of medium-sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum. J Comp Neurol. 2007;500:908-22 pubmed
immunohistochemistry - frozen section; mouse; 1:500	Xu X, Roby K, Callaway E. Mouse cortical inhibitory neuron type that coexpresses somatostatin and calretinin. J Comp Neurol. 2006;499:144-60 pubmed
immunohistochemistry - frozen section; rat; 1:20000	Loopuijt L, Zahm D. Synaptologic and fine structural features distinguishing a subset of basal forebrain cholinergic neurons embedded in the dense intrinsic fiber network of the caudal extended amygdala. J Comp Neurol. 2006;498:93-111 pubmed
immunohistochemistry - frozen section; rhesus macaque; 1:500	Imura K, Rockland K. Long-range interneurons within the medial pulvinar nucleus of macaque monkeys. J Comp Neurol. 2006;498:649-66 pubmed
immunocytochemistry; rat; 1:2000	King A, Chung R, Vickers J, Dickson T. Localization of glutamate receptors in developing cortical neurons in culture and relationship to susceptibility to excitotoxicity. J Comp Neurol. 2006;498:277-94 pubmed
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product information

Catalog Number :

MAB5406

Subcategory :

Neuroscience

Product Name :

Anti-GAD67 Antibody, clone 1G10.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q99259

Host Name :

Mouse

Antigen :

GAD67

Clone :

1G10.2

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-GAD67 Antibody, clone 1G10.2

Cross Reactivity :

Human;Rat;Mouse

Background :

Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.

ALT Names :

Glutamate Decarboxylase 67kDa Isoform; Glutamate Decarboxylase 1; Glutamic acid decarboxylase 1

Immunogen :

Recombinant GAD67 protein

Specificity :

Reacts with the 67kDa isoform of Glutamate Decarboxylase (GAD67) of rat, mouse and human origins, other species not yet tested. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate when compared to blot probed with AB1511 that reacts with both GAD65 & GAD67.

Package Size :

100 µg

Uses :

Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.