This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Nitrotyrosine Antibody, clone 2A8.2
catalog :
MAB5404
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2A8.2
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 34
Reference
Chauhan P, Sheng W, Hu S, Prasad S, Lokensgard J. Nitrosative damage during retrovirus infection-induced neuropathic pain. J Neuroinflammation. 2018;15:66 pubmed publisher
Chang J, Chow L, Dismuke W, Ethier C, Stevens M, Stamer W, et al. Peptide-Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite-Mediated Nitration. Adv Healthc Mater. 2017;6: pubmed publisher
Kruzliak P, Sabo J, Zulli A. Endothelial endoplasmic reticulum and nitrative stress in endothelial dysfunction in the atherogenic rabbit model. Acta Histochem. 2015;117:762-6 pubmed publisher
Kovács M, Kiss A, Gönczi M, Miskolczi G, Seprényi G, Kaszaki J, et al. Effect of sodium nitrite on ischaemia and reperfusion-induced arrhythmias in anaesthetized dogs: is protein S-nitrosylation involved?. PLoS ONE. 2015;10:e0122243 pubmed publisher
Rodríguez González G, Vega C, Boeck L, Vázquez M, Bautista C, Reyes Castro L, et al. Maternal obesity and overnutrition increase oxidative stress in male rat offspring reproductive system and decrease fertility. Int J Obes (Lond). 2015;39:549-56 pubmed publisher
Davies C, Dey N, Negrette O, Parada L, Basombrio M, Garg N. Hepatotoxicity in mice of a novel anti-parasite drug candidate hydroxymethylnitrofurazone: a comparison with Benznidazole. PLoS Negl Trop Dis. 2014;8:e3231 pubmed publisher
Huang E, Wang F, Chen Y, Chen Y, Wang C, Lin I, et al. Amifostine alleviates radiation-induced lethal small bowel damage via promotion of 14-3-3σ-mediated nuclear p53 accumulation. Oncotarget. 2014;5:9756-69 pubmed
Marín T, Contreras P, Castro J, Chamorro D, Balboa E, Bosch Morató M, et al. Vitamin E dietary supplementation improves neurological symptoms and decreases c-Abl/p73 activation in Niemann-Pick C mice. Nutrients. 2014;6:3000-17 pubmed publisher
Liaw N, Fox J, Siddiqui A, Meng H, Kolega J. Endothelial nitric oxide synthase and superoxide mediate hemodynamic initiation of intracranial aneurysms. PLoS ONE. 2014;9:e101721 pubmed publisher
Noonan K, Ghosh N, Rudraraju L, Bui M, Borrello I. Targeting immune suppression with PDE5 inhibition in end-stage multiple myeloma. Cancer Immunol Res. 2014;2:725-31 pubmed publisher
Das Gupta S, So J, Wall B, Wahler J, Smolarek A, Sae Tan S, et al. Tocopherols inhibit oxidative and nitrosative stress in estrogen-induced early mammary hyperplasia in ACI rats. Mol Carcinog. 2015;54:916-25 pubmed publisher
Cheng C, Lin J, Tang N, Kao S, Hsieh C. Electroacupuncture-like stimulation at the Baihui (GV20) and Dazhui (GV14) acupoints protects rats against subacute-phase cerebral ischemia-reperfusion injuries by reducing S100B-mediated neurotoxicity. PLoS ONE. 2014;9:e91426 pubmed publisher
Miyata R, Hiraiwa K, Cheng J, Bai N, Vincent R, Francis G, et al. Statins attenuate the development of atherosclerosis and endothelial dysfunction induced by exposure to urban particulate matter (PM10). Toxicol Appl Pharmacol. 2013;272:1-11 pubmed publisher
Phan O, Maillard M, Peregaux C, Mordasini D, Stehle J, Funk F, et al. PA21, a new iron-based noncalcium phosphate binder, prevents vascular calcification in chronic renal failure rats. J Pharmacol Exp Ther. 2013;346:281-9 pubmed publisher
Koyama Y, Taura K, Hatano E, Tanabe K, Yamamoto G, Nakamura K, et al. Effects of oral intake of hydrogen water on liver fibrogenesis in mice. Hepatol Res. 2014;44:663-677 pubmed publisher
Wu L, Li X, Zhu H, Xu P, Gao X. A prescribed Chinese herbal medicine improves glucose profile and ameliorates oxidative stress in Goto-Kakisaki rats fed with high fat diet. PLoS ONE. 2013;8:e60262 pubmed publisher
Leonelli M, Martins D, Britto L. Retinal cell death induced by TRPV1 activation involves NMDA signaling and upregulation of nitric oxide synthases. Cell Mol Neurobiol. 2013;33:379-92 pubmed publisher
Wan X, Gupta S, Zago M, Davidson M, Dousset P, Amoroso A, et al. Defects of mtDNA replication impaired mitochondrial biogenesis during Trypanosoma cruzi infection in human cardiomyocytes and chagasic patients: the role of Nrf1/2 and antioxidant response. J Am Heart Assoc. 2012;1:e003855 pubmed publisher
Petre B, Ulrich M, Stumbaum M, Bernevic B, Moise A, Doring G, et al. When is mass spectrometry combined with affinity approaches essential? A case study of tyrosine nitration in proteins. J Am Soc Mass Spectrom. 2012;23:1831-40 pubmed publisher
Takakusa H, Mohar I, Kavanagh T, Kelly E, Kaspera R, Nelson S. Protein tyrosine nitration of mitochondrial carbamoyl phosphate synthetase 1 and its functional consequences. Biochem Biophys Res Commun. 2012;420:54-60 pubmed publisher
Juhász L, Kiss A, Nyeso E, Kovacs M, Seprényi G, Kaszaki J, et al. Is there a trigger role of peroxynitrite in the anti-arrhythmic effect of ischaemic preconditioning and peroxynitrite infusion?. Eur J Pharmacol. 2011;667:306-13 pubmed publisher
Dr Guşanu M, Petre B, Przybylski M. Epitope motif of an anti-nitrotyrosine antibody specific for tyrosine-nitrated peptides revealed by a combination of affinity approaches and mass spectrometry. J Pept Sci. 2011;17:184-91 pubmed publisher
Leonelli M, Martins D, Britto L. TRPV1 receptors are involved in protein nitration and Müller cell reaction in the acutely axotomized rat retina. Exp Eye Res. 2010;91:755-68 pubmed publisher
Dragusanu M, Petre B, Slamnoiu S, Vlad C, Tu T, Przybylski M. On-line bioaffinity-electrospray mass spectrometry for simultaneous detection, identification, and quantification of protein-ligand interactions. J Am Soc Mass Spectrom. 2010;21:1643-8 pubmed publisher
Cheng C, Su S, Tang N, Ho T, Lo W, Hsieh C. Ferulic acid inhibits nitric oxide-induced apoptosis by enhancing GABA(B1) receptor expression in transient focal cerebral ischemia in rats. Acta Pharmacol Sin. 2010;31:889-99 pubmed publisher
Zheng B, Zheng T, Wang L, Chen X, Shi C, Zhao S. Aminoguanidine inhibition of iNOS activity ameliorates cerebral vasospasm after subarachnoid hemorrhage in rabbits via restoration of dysfunctional endothelial cells. J Neurol Sci. 2010;295:97-103 pubmed publisher
Zhan X, Desiderio D. Mass spectrometric identification of in vivo nitrotyrosine sites in the human pituitary tumor proteome. Methods Mol Biol. 2009;566:137-63 pubmed publisher
Rai S, Hare D, Zulli A. A physiologically relevant atherogenic diet causes severe endothelial dysfunction within 4 weeks in rabbit. Int J Exp Pathol. 2009;90:598-604 pubmed publisher
Kupai K, Csonka C, Fekete V, Odendaal L, van Rooyen J, Marais D, et al. Cholesterol diet-induced hyperlipidemia impairs the cardioprotective effect of postconditioning: role of peroxynitrite. Am J Physiol Heart Circ Physiol. 2009;297:H1729-35 pubmed publisher
Huang E, Wang F, Lin I, Yang K. Aminoguanidine alleviates radiation-induced small-bowel damage through its antioxidant effect. Int J Radiat Oncol Biol Phys. 2009;74:237-44 pubmed publisher
Kiss A, Juhasz L, Huliák I, Vegh A. Peroxynitrite decreases arrhythmias induced by ischaemia reperfusion in anaesthetized dogs, without involving mitochondrial KATP channels. Br J Pharmacol. 2008;155:1015-24 pubmed publisher
Siu P, Pistilli E, Alway S. Age-dependent increase in oxidative stress in gastrocnemius muscle with unloading. J Appl Physiol (1985). 2008;105:1695-705 pubmed publisher
Takahashi Y, Iida K, Takahashi K, Yoshioka S, Fukuoka H, Takeno R, et al. Growth hormone reverses nonalcoholic steatohepatitis in a patient with adult growth hormone deficiency. Gastroenterology. 2007;132:938-43 pubmed
Siu P, Alway S. Deficiency of the Bax gene attenuates denervation-induced apoptosis. Apoptosis. 2006;11:967-81 pubmed
product information
Catalog Number :
MAB5404
Subcategory :
Neuroscience
Product Name :
Anti-Nitrotyrosine Antibody, clone 2A8.2
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
Nitrotyrosine
Clone :
2A8.2
Conjugate :
Purified
Isotype :
IgG2b
Product Description :
Anti-Nitrotyrosine Antibody, clone 2A8.2
Cross Reactivity :
All
Background :
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Immunogen :
Nitrated KLH.
Specificity :
Reacts with nitrotyrosine.
Package Size :
100 μg
Uses :
Immunohistochemistry (Paraffin);Western Blotting
Storage :
Stable for 1 year at 2-8ºC from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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