Hypoxia Inducible Factor 1 α Antibody, clone H1α67 | EMD Millipore MAB5382 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Hypoxia Inducible Factor 1 α Antibody, clone H1α67

catalog :

MAB5382

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H1alpha67

The same clone is also sold as:

Abnova: MAB1892
Abcam: ab1
LifeSpan Biosciences: LS-B110, LS-C140805, LS-C87584, LS-C140780, LS-C140804, LS-C140803, LS-C140806, LS-C2557, LS-C140777, LS-C140802, LS-C140778, LS-C140776, LS-C87583
Novus Biologicals: NB100-105, NBP2-80760, NB100-123
Invitrogen: MA5-16010, MA5-16008, MA1-16511, MA5-16009, MA1-16504, MA1-46457
OriGene: TA309691

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation

citations: 25

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4a	Piao L, Yang Z, Feng Y, Zhang C, Cui C, Xuan Y. LETM1 is a potential biomarker of prognosis in lung non-small cell carcinoma. BMC Cancer. 2019;19:898 pubmed publisher
chromatin immunoprecipitation; rat western blot; rat; 1:1000; fig 5	Formisano L, Guida N, Valsecchi V, Cantile M, Cuomo O, Vinciguerra A, et al. Sp3/REST/HDAC1/HDAC2 Complex Represses and Sp1/HIF-1/p300 Complex Activates ncx1 Gene Transcription, in Brain Ischemia and in Ischemic Brain Preconditioning, by Epigenetic Mechanism. J Neurosci. 2015;35:7332-48 pubmed publisher
	Liu W, Zhao Z, Liu Y, Pan H, Lin L. Weipiling ameliorates gastric precancerous lesions in Atp4a-/- mice. BMC Complement Altern Med. 2019;19:318 pubmed publisher
	Wendeln A, Degenhardt K, Kaurani L, Gertig M, Ulas T, Jain G, et al. Innate immune memory in the brain shapes neurological disease hallmarks. Nature. 2018;556:332-338 pubmed publisher
	Pahima H, Reina S, Tadmor N, Dadon Klein D, Shteinfer Kuzmine A, Mazure N, et al. Hypoxic-induced truncation of voltage-dependent anion channel 1 is mediated by both asparagine endopeptidase and calpain 1 activities. Oncotarget. 2018;9:12825-12841 pubmed publisher
	Owen J, McEwan C, Nesbitt H, Bovornchutichai P, Averre R, Borden M, et al. Reducing Tumour Hypoxia via Oral Administration of Oxygen Nanobubbles. PLoS ONE. 2016;11:e0168088 pubmed publisher
	de Wit R, MujiÄ‡ DeliÄ‡ A, van Senten J, Fraile Ramos A, Siderius M, Smit M. Human cytomegalovirus encoded chemokine receptor US28 activates the HIF-1Î±/PKM2 axis in glioblastoma cells. Oncotarget. 2016;7:67966-67985 pubmed publisher
	Ivanina A, Nesmelova I, Leamy L, Sokolov E, Sokolova I. Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs. J Exp Biol. 2016;219:1659-74 pubmed publisher
	Seino H, Ono S, Miura H, Morohashi S, Wu Y, Tsushima F, et al. Hypoxia is important in F?18 FDG accumulation in thecoma?fibroma tumors on F?18 FDG PET/CT scans. Mol Med Rep. 2016;13:3821-7 pubmed publisher
	Micucci C, Matacchione G, Valli D, Orciari S, Catalano A. HIF2Î± is involved in the expansion of CXCR4-positive cancer stem-like cells in renal cell carcinoma. Br J Cancer. 2015;113:1178-85 pubmed publisher
	To C, Farnsworth R, Vail M, Chheang C, Gargett C, Murone C, et al. Hypoxia-controlled EphA3 marks a human endometrium-derived multipotent mesenchymal stromal cell that supports vascular growth. PLoS ONE. 2014;9:e112106 pubmed publisher
	Dong M, Fan X, Chen Z, Wang T, Li X, Chen J, et al. Aberrant expression of enhancer of zeste homologue 2, correlated with HIF-1?, refines relapse risk and predicts poor outcome for breast cancer. Oncol Rep. 2014;32:1101-7 pubmed publisher
	Dong M, Wan X, Yuan Z, Wei L, Fan X, Wang T, et al. Low expression of Beclin 1 and elevated expression of HIF-1? refine distant metastasis risk and predict poor prognosis of ER-positive, HER2-negative breast cancer. Med Oncol. 2013;30:355 pubmed publisher
	Xu X, Lv Y, Gu W, Tang L, Wei J, Zhang L, et al. Epigenetics of hypoxic pulmonary arterial hypertension following intrauterine growth retardation rat: epigenetics in PAH following IUGR. Respir Res. 2013;14:20 pubmed publisher
	Wu X, Chen J, Cao Q, Dong M, Lin Q, Fan X, et al. Beclin 1 activation enhances chemosensitivity and predicts a favorable outcome for primary duodenal adenocarcinoma. Tumour Biol. 2013;34:713-22 pubmed publisher
	Shi Q, Yang P, Xu X, Chen X, Qi C, Zhang J, et al. Neuroprotection of neurotrophin-3 against focal cerebral ischemia/reperfusion injury is regulated by hypoxia-responsive element in rats. Neuroscience. 2012;222:1-9 pubmed publisher
	Wan X, Zhao Y, Fan X, Cai H, Zhang Y, Chen M, et al. Molecular prognostic prediction for locally advanced nasopharyngeal carcinoma by support vector machine integrated approach. PLoS ONE. 2012;7:e31989 pubmed publisher
	Lakemeier S, Schofer M, Foltz L, Schmid R, Efe T, Rohlfs J, et al. Expression of hypoxia-inducible factor-1?, vascular endothelial growth factor, and matrix metalloproteinases 1, 3, and 9 in hypertrophied ligamentum flavum. J Spinal Disord Tech. 2013;26:400-6 pubmed publisher
	Husted R, Lu H, Sigmund R, Stokes J. Oxygen regulation of the epithelial Na channel in the collecting duct. Am J Physiol Renal Physiol. 2011;300:F412-24 pubmed publisher
	Lakemeier S, Reichelt J, Patzer T, Fuchs Winkelmann S, Paletta J, Schofer M. The association between retraction of the torn rotator cuff and increasing expression of hypoxia inducible factor 1? and vascular endothelial growth factor expression: an immunohistological study. BMC Musculoskelet Disord. 2010;11:230 pubmed publisher
	De Gasperi R, Sosa M, Dracheva S, Elder G. Presenilin-1 regulates induction of hypoxia inducible factor-1?: altered activation by a mutation associated with familial Alzheimer's disease. Mol Neurodegener. 2010;5:38 pubmed publisher
	Ferrari D, Nesic O, Perez Polo J. Oxygen resuscitation does not ameliorate neonatal hypoxia/ischemia-induced cerebral edema. J Neurosci Res. 2010;88:2056-65 pubmed publisher
	Bianco C, Cotten C, Lonardo E, Strizzi L, Baraty C, Mancino M, et al. Cripto-1 is required for hypoxia to induce cardiac differentiation of mouse embryonic stem cells. Am J Pathol. 2009;175:2146-58 pubmed publisher
	Kolev Y, Uetake H, Takagi Y, Sugihara K. Lactate dehydrogenase-5 (LDH-5) expression in human gastric cancer: association with hypoxia-inducible factor (HIF-1alpha) pathway, angiogenic factors production and poor prognosis. Ann Surg Oncol. 2008;15:2336-44 pubmed publisher
	Picchio M, Beck R, Haubner R, Seidl S, Machulla H, Johnson T, et al. Intratumoral spatial distribution of hypoxia and angiogenesis assessed by 18F-FAZA and 125I-Gluco-RGD autoradiography. J Nucl Med. 2008;49:597-605 pubmed publisher

product information

Catalog Number :

MAB5382

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Hypoxia Inducible Factor 1 α Antibody, clone H1α67

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16665

Host Name :

Mouse

Antigen :

Hypoxia Inducible Factor 1 α

Clone :

H1alpha67

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-Hypoxia Inducible Factor 1 α Antibody, clone H1α67

Cross Reactivity :

Ferret;Human;Mouse;Rat;Sheep

Background :

HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis, the posttranslational modification by prolyl hydroxylation as a key regulatory event that targets HIF-alpha subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. The transcriptional complex is composed of an alpha-beta heterodimer; HIF-beta being a constitutive nuclear protein that dimerises with oxygen regulated HIF-alpha subunits. In normoxia, 4-hydroxylation of human HIF-alpha at Pro402 or Pro564 by a set of HIF prolyl hydroxylase isoenzymes (PHD 1-3) mediates HIF1-alpha recognition by von Hippel-Lindau ubiquitin ligase complex leading to its proteasomal destruction. In hypoxia (deprivation of oxygen), lack of hydroxylase activity enables HIF-alpha subunits to escape destruction and become transcriptionally active. Thus HIF hydroxylases provide a focus for understanding cellular responses to hypoxia and target for therapeutic manipulation. There are several HIF factors, which include HIF 1-alpha, HIF 1-beta, HIF 2-alpha. HIF 1-alpha is an 812 a.a. protein in rat and 836 a.a. long in mouse and human. A master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. It is ubiquitous in expression as cytoplasmic in normoxia, nuclear translocation in response to hypoxia.

ALT Names :

HIF-1 alpha; ARNT Interacting Protein; MOP1

Immunogen :

Fusion protein from amino acids 432-528 of human HIF-1alpha.

Specificity :

Recognizes Hypoxia inducible factor-1alpha (HIF-1alpha).

Package Size :

100 μg

Uses :

Electrophoretic Mobility Shift Assay;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.