This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4
catalog :
MAB5356
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Rho 1D4

The same clone is also sold as:
reactivity :
American opossums, human, mouse, rat, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 47
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4e1
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1e1
Chen F, Liu X, Chen Y, Liu J, Lu H, Wang W, et al. Sphere-induced reprogramming of RPE cells into dual-potential RPE stem-like cells. EBioMedicine. 2020;52:102618 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5c
Findlay A, McKie L, Keighren M, Clementson Mobbs S, Sanchez Pulido L, Wells S, et al. Fam151b, the mouse homologue of C.elegans menorin gene, is essential for retinal function. Sci Rep. 2020;10:437 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3a
Datta P, Hendrickson B, Brendalen S, Ruffcorn A, Seo S. The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. J Biol Chem. 2019;294:19119-19136 pubmed publisher
  • immunohistochemistry; mouse; 1:250; loading ...; fig 6a
Rubio Fernández M, Uribe M, Vicente Tejedor J, Germain F, Susín Lara C, Quereda C, et al. Impairment of photoreceptor ribbon synapses in a novel Pomt1 conditional knockout mouse model of dystroglycanopathy. Sci Rep. 2018;8:8543 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:5000; loading ...; tbl 2
  • western blot; mouse; loading ...; fig 4
Chaney S, Mukherjee S, Giddabasappa A, Rueda E, Hamilton W, Johnson J, et al. Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation. Mol Vis. 2016;22:1468-1489 pubmed
  • immunohistochemistry - frozen section; mouse; 1:100; fig 1
Su X, Tan Q, Parikh B, Tan A, Mehta M, Sia Wey Y, et al. Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina. Invest Ophthalmol Vis Sci. 2016;57:3397-408 pubmed publisher
  • immunohistochemistry; mouse; 1:5000; fig 3
Jain V, Srivastava I, Palchaudhuri S, Goel M, Sinha Mahapatra S, Dhingra N. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS ONE. 2016;11:e0157226 pubmed publisher
  • immunocytochemistry; human; 1:200; tbl 1
de Souza C, Nivison Smith L, Christie D, Polkinghorne P, McGhee C, Kalloniatis M, et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp Eye Res. 2016;150:135-48 pubmed publisher
  • immunohistochemistry; rat; 1:100; fig s1
Bakondi B, Lv W, Lu B, Jones M, Tsai Y, Kim K, et al. In Vivo CRISPR/Cas9 Gene Editing Corrects Retinal Dystrophy in the S334ter-3 Rat Model of Autosomal Dominant Retinitis Pigmentosa. Mol Ther. 2016;24:556-63 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 1e
  • western blot; mouse; 1:1000; loading ...; fig 1f
Grishchuk Y, Stember K, Matsunaga A, Olivares A, CRUZ N, King V, et al. Retinal Dystrophy and Optic Nerve Pathology in the Mouse Model of Mucolipidosis IV. Am J Pathol. 2016;186:199-209 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:500; loading ...; fig 8f
Pinilla I, Fernández Sánchez L, Segura F, Sánchez Cano A, Tamarit J, Fuentes Broto L, et al. Long time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence. Exp Eye Res. 2016;150:122-34 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 2
Zhao L, Zabel M, Wang X, Ma W, Shah P, Fariss R, et al. Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Mol Med. 2015;7:1179-97 pubmed publisher
  • immunohistochemistry; mouse; fig 4
Du M, Otalora L, Martin A, Moiseyev G, Vanlandingham P, Wang Q, et al. Transgenic Mice Overexpressing Serum Retinol-Binding Protein Develop Progressive Retinal Degeneration through a Retinoid-Independent Mechanism. Mol Cell Biol. 2015;35:2771-89 pubmed publisher
  • western blot; human; 1:2000
Berger A, Lorain S, Joséphine C, Desrosiers M, Peccate C, Voit T, et al. Repair of rhodopsin mRNA by spliceosome-mediated RNA trans-splicing: a new approach for autosomal dominant retinitis pigmentosa. Mol Ther. 2015;23:918-930 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:3000
  • western blot; mouse; 1:3000
Zhou Z, Doggett T, Sene A, Apte R, Ferguson T. Autophagy supports survival and phototransduction protein levels in rod photoreceptors. Cell Death Differ. 2015;22:488-98 pubmed publisher
  • immunohistochemistry; human; 1:200
Bray A, Cevallos R, Gazarian K, Lamas M. Human dental pulp stem cells respond to cues from the rat retina and differentiate to express the retinal neuronal marker rhodopsin. Neuroscience. 2014;280:142-55 pubmed publisher
  • immunocytochemistry; human; 1:1000
  • western blot; human; 1:5000
Molday L, Molday R. 1D4: a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins. Methods Mol Biol. 2014;1177:1-15 pubmed publisher
  • immunohistochemistry - free floating section; American opossums
Vlahos L, Knott B, Valter K, Hemmi J. Photoreceptor topography and spectral sensitivity in the common brushtail possum (Trichosurus vulpecula). J Comp Neurol. 2014;522:3423-36 pubmed publisher
Kotov V, Bartels K, Veith K, Josts I, Subhramanyam U, Günther C, et al. High-throughput stability screening for detergent-solubilized membrane proteins. Sci Rep. 2019;9:10379 pubmed publisher
Saeed U, Kim J, Piracha Z, Kwon H, Jung J, Chwae Y, et al. Parvulin 14 and Parvulin 17 Bind to HBx and cccDNA and Upregulate Hepatitis B Virus Replication from cccDNA to Virion in an HBx-Dependent Manner. J Virol. 2019;93: pubmed publisher
Zhang F, Jara Oseguera A, Chang T, Bae C, Hanson S, Swartz K. Heat activation is intrinsic to the pore domain of TRPV1. Proc Natl Acad Sci U S A. 2018;115:E317-E324 pubmed publisher
Pyakurel A, Balmer D, Saba El Leil M, Kizilyaprak C, Daraspe J, Humbel B, et al. Loss of Extracellular Signal-Regulated Kinase 1/2 in the Retinal Pigment Epithelium Leads to RPE65 Decrease and Retinal Degeneration. Mol Cell Biol. 2017;37: pubmed publisher
Vargas A, Kim H, Baral E, Yu W, CRAFT C, Lee E. Protective effect of clusterin on rod photoreceptor in rat model of retinitis pigmentosa. PLoS ONE. 2017;12:e0182389 pubmed publisher
Shen W, Zhu L, Lee S, Chung S, Gillies M. Involvement of NT3 and P75(NTR) in photoreceptor degeneration following selective Müller cell ablation. J Neuroinflammation. 2013;10:137 pubmed publisher
Reyes Aguirre L, Ferraro S, Quintero H, Sánchez Serrano S, Gomez Montalvo A, Lamas M. Glutamate-induced epigenetic and morphological changes allow rat Müller cell dedifferentiation but not further acquisition of a photoreceptor phenotype. Neuroscience. 2013;254:347-60 pubmed publisher
Wyatt M, Tsai J, Mishra S, Campos M, Jaworski C, Fariss R, et al. Interaction of complement factor h and fibulin3 in age-related macular degeneration. PLoS ONE. 2013;8:e68088 pubmed publisher
Li J, Liu K, Liu Y, Xu Y, Zhang F, Yang H, et al. Exosomes mediate the cell-to-cell transmission of IFN-?-induced antiviral activity. Nat Immunol. 2013;14:793-803 pubmed publisher
Zhao Y, Yu B, Xiang Y, Han X, Xu Y, So K, et al. Changes in retinal morphology, electroretinogram and visual behavior after transient global ischemia in adult rats. PLoS ONE. 2013;8:e65555 pubmed publisher
Powner M, Gillies M, Zhu M, Vevis K, Hunyor A, Fruttiger M. Loss of Müller's cells and photoreceptors in macular telangiectasia type 2. Ophthalmology. 2013;120:2344-52 pubmed publisher
Tiwari S, Hudson S, Gattone V, Miller C, Chernoff E, Belecky Adams T. Meckelin 3 is necessary for photoreceptor outer segment development in rat Meckel syndrome. PLoS ONE. 2013;8:e59306 pubmed publisher
Goel M, Dhingra N. Müller glia express rhodopsin in a mouse model of inherited retinal degeneration. Neuroscience. 2012;225:152-61 pubmed publisher
Yin J, Brocher J, Linder B, Hirmer A, Sundaramurthi H, Fischer U, et al. The 1D4 antibody labels outer segments of long double cone but not rod photoreceptors in zebrafish. Invest Ophthalmol Vis Sci. 2012;53:4943-51 pubmed publisher
Zeng R, Zhang Y, Shi F, Kong F. A novel experimental mouse model of retinal detachment: complete functional and histologic recovery of the retina. Invest Ophthalmol Vis Sci. 2012;53:1685-95 pubmed publisher
Nakajima E, Hammond K, Rosales J, Shearer T, Azuma M. Calpain, not caspase, is the causative protease for hypoxic damage in cultured monkey retinal cells. Invest Ophthalmol Vis Sci. 2011;52:7059-67 pubmed publisher
Fabiano A, Panfoli I, Calzia D, Bruschi M, Ravera S, Bachi A, et al. Catalytic properties of the retinal rod outer segment disk ADP-ribosyl cyclase. Vis Neurosci. 2011;28:121-8 pubmed publisher
Giddabasappa A, Hamilton W, Chaney S, Xiao W, Johnson J, Mukherjee S, et al. Low-level gestational lead exposure increases retinal progenitor cell proliferation and rod photoreceptor and bipolar cell neurogenesis in mice. Environ Health Perspect. 2011;119:71-7 pubmed publisher
Ohgane K, Dodo K, Hashimoto Y. Retinobenzaldehydes as proper-trafficking inducers of folding-defective P23H rhodopsin mutant responsible for retinitis pigmentosa. Bioorg Med Chem. 2010;18:7022-8 pubmed publisher
Powner M, Gillies M, Tretiach M, Scott A, Guymer R, Hageman G, et al. Perifoveal müller cell depletion in a case of macular telangiectasia type 2. Ophthalmology. 2010;117:2407-16 pubmed publisher
Zhong M, Molday R. Binding of retinoids to ABCA4, the photoreceptor ABC transporter associated with Stargardt macular degeneration. Methods Mol Biol. 2010;652:163-76 pubmed publisher
Sato H, Suzuki T, Ikeda K, Masuya H, Sezutsu H, Kaneda H, et al. A monogenic dominant mutation in Rom1 generated by N-ethyl-N-nitrosourea mutagenesis causes retinal degeneration in mice. Mol Vis. 2010;16:378-91 pubmed
Panfoli I, Calzia D, Ravera S, Bianchini P, Diaspro A. Immunochemical or fluorescent labeling of vesicular subcellular fractions for microscopy imaging. Microsc Res Tech. 2010;73:1086-90 pubmed publisher
Cammas L, Trensz F, Jellali A, Ghyselinck N, Roux M, Dolle P. Retinoic acid receptor (RAR)-alpha is not critically required for mediating retinoic acid effects in the developing mouse retina. Invest Ophthalmol Vis Sci. 2010;51:3281-90 pubmed publisher
Sun X, Jiang R, Zhang Y, Chen M, Xiang P, Qi Y, et al. Gene expression and differentiation characteristics in mice E13.5 and E17.5 neural retinal progenitors. Mol Vis. 2009;15:2503-14 pubmed
Haruta M, Bush R, Kjellstrom S, Vijayasarathy C, Zeng Y, Le Y, et al. Depleting Rac1 in mouse rod photoreceptors protects them from photo-oxidative stress without affecting their structure or function. Proc Natl Acad Sci U S A. 2009;106:9397-402 pubmed publisher
Nagar S, Krishnamoorthy V, Cherukuri P, Jain V, Dhingra N. Early remodeling in an inducible animal model of retinal degeneration. Neuroscience. 2009;160:517-29 pubmed publisher
Zacks D, Boehlke C, Richards A, Zheng Q. Role of the Fas-signaling pathway in photoreceptor neuroprotection. Arch Ophthalmol. 2007;125:1389-95 pubmed
Ravera S, Musante L, Calzia D, Panfoli I, Bruschi M, Candiano G, et al. Expression of adenylate kinase 1 in bovine retinal cytosol. Curr Eye Res. 2007;32:249-57 pubmed
product information
Catalog Number :
MAB5356
Subcategory :
Neuroscience
Product Name :
Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P08100
Host Name :
Mouse
Antigen :
Rhodopsin
Clone :
Rho 1D4
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4
Cross Reactivity :
Vertebrates
Immunogen :
Bovine rhodopsin
Specificity :
Recognizes Rhodopsin. Reacts with both native and recombinant protein. No known reactivity to other proteins.
Package Size :
100 μg
Uses :
Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
  • • Cancer
  • • Nuclear Signaling
  • • Apoptosis
  • • Cardiovascular Disease
  • • Developmental Biology
  • • Metabolic Disease
  • • Neurodegenerative Disease
  • • Cell Biology
View all the new antibodies at www.emdmillipore.com.