Notch 1 Antibody, clone mN1A | EMD Millipore MAB5352 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Notch 1 Antibody, clone mN1A

catalog :

MAB5352

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mN1A

The same clone is also sold as:

BD Biosciences: 552768, 552466
Exbio: 11-651-C100, 1P-651-C025, 1A-651-C025, 1P-651-C100, 1A-651-C100, 11-651-C025
BioLegend: 629105, 629106
Invitrogen: 14-5785-81, 14-5785-95, MA1-81888, 14-5785-37, 41-3500, 12-5785-82, MA5-16862
LifeSpan Biosciences: LS-C204395, LS-C359244, LS-C44817, LS-C204456, LS-C188303, LS-C204312, LS-C44818
OriGene: SM2297PS, TA336494
Novus Biologicals: NBP2-80884, NB100-78486
MilliporeSigma: SAB4700742, N6786
Abcam: ab128076
Bio-Rad: MCA2410GA, MCA2410PE, MCA2410F, MCA2410A647
Santa Cruz Biotechnology: sc-32745

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; fig 4	Gökbuget D, Pereira J, Bachofner S, Marchais A, Ciaudo C, Stoffel M, et al. The Lin28/let-7 axis is critical for myelination in the peripheral nervous system. Nat Commun. 2015;6:8584 pubmed publisher
immunocytochemistry; mouse; 1:200	Feng Y, Liang G, Pan B, Qin X, Zhang X, Chen C, et al. Notch pathway regulates female germ cell meiosis progression and early oogenesis events in fetal mouse. Cell Cycle. 2014;13:782-91 pubmed publisher
	Kim J, Kim J, Lee J, Park M. Oct4 suppresses IR‑induced premature senescence in breast cancer cells through STAT3- and NF‑κB-mediated IL‑24 production. Int J Oncol. 2018;53:47-58 pubmed publisher
	Li S, Wang R, Wang Y, Li H, Zheng J, Duan R, et al. Receptors of the Notch signaling pathway are associated with hemorrhage of brain arteriovenous malformations. Mol Med Rep. 2014;9:2233-8 pubmed publisher
	Ressel L, Else R, Poli A, Argyle D. Aberrant subcellular immunolocalization of NOTCH-1 activated intracellular domain in feline mammary tumours. J Comp Pathol. 2014;150:366-72 pubmed publisher
	Pettersson S, Sczaniecka M, McLaren L, Russell F, Gladstone K, Hupp T, et al. Non-degradative ubiquitination of the Notch1 receptor by the E3 ligase MDM2 activates the Notch signalling pathway. Biochem J. 2013;450:523-36 pubmed publisher
	Guo F, Lv S, Lou Y, Tu W, Liao W, Wang Y, et al. Bone marrow stromal cells enhance the angiogenesis in ischaemic cortex after stroke: involvement of notch signalling. Cell Biol Int. 2012;36:997-1004 pubmed publisher
	Sczaniecka M, Gladstone K, Pettersson S, McLaren L, Huart A, Wallace M. MDM2 protein-mediated ubiquitination of numb protein: identification of a second physiological substrate of MDM2 that employs a dual-site docking mechanism. J Biol Chem. 2012;287:14052-68 pubmed publisher
	Iulianella A, Sharma M, Vanden Heuvel G, Trainor P. Cux2 functions downstream of Notch signaling to regulate dorsal interneuron formation in the spinal cord. Development. 2009;136:2329-34 pubmed publisher
	Fischer D, Van Dijk R, Sluijs J, Nair S, Racchi M, Levelt C, et al. Activation of the Notch pathway in Down syndrome: cross-talk of Notch and APP. FASEB J. 2005;19:1451-8 pubmed

product information

Catalog Number :

MAB5352

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Notch 1 Antibody, clone mN1A

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P46531

Host Name :

Mouse

Antigen :

Notch 1

Clone :

mN1A

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Notch 1 Antibody, clone mN1A

Cross Reactivity :

Mouse;Rat;Feline

Background :

Notch1 consists of three domains. The extracellular domain containing 36 epidermal growth factor (EGF) repeats and 3 lin-12/Notch repeats (LNR) and functions as a receptor in cell-cell interactions. There is a single transmembrane domain (TM). The intracellular domain contains six cdc10/ankyrin repeats (cdc10) and the newly defined Notch cytokine response (NCR) region, which contains a putative bipartite NLS (nuclear localization signal).

Immunogen :

Cdc10-NCR region of mouse Notch1 expressed as a GST fusion protein.

Specificity :

Recognizes Notch1, intracellular domain. The antibody has a high affinity for the "activated" intracellular form of Notch1, but a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms. No reactivity with Notch2, 3 or 4.

Package Size :

100 μg

Uses :

Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.