This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Nestin Antibody, clone 10C2
catalog :
MAB5326
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
reactivity :
giant panda, human, mouse, rat, chicken, pigs , rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
citations: 233
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; loading ...; fig s2a
Gao J, Wu Y, He D, Zhu X, Li H, Liu H, et al. Anti-aging effects of Ribes meyeri anthocyanins on neural stem cells and aging mice. Aging (Albany NY). 2020;12:17738-17753 pubmed publisher
  • other; human; 1:100; loading ...
Leelatian N, Sinnaeve J, Mistry A, Barone S, Brockman A, Diggins K, et al. Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2d
  • western blot; human; 1:1000; loading ...; fig 1a
Huang C, Lu S, Huang T, Huang B, Sun H, Yang S, et al. FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells. Theranostics. 2020;10:2817-2831 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig s15e
Kielkowski P, Buchsbaum I, Kirsch V, Bach N, Drukker M, Cappello S, et al. FICD activity and AMPylation remodelling modulate human neurogenesis. Nat Commun. 2020;11:517 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1d
Marin Navarro A, Pronk R, van der Geest A, Oliynyk G, Nordgren A, Arsenian Henriksson M, et al. p53 controls genomic stability and temporal differentiation of human neural stem cells and affects neural organization in human brain organoids. Cell Death Dis. 2020;11:52 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig s3
  • immunocytochemistry; human; 1:100; loading ...; fig s6
Skoda J, Neradil J, Staniczkova Zambo I, Nunukova A, Macsek P, Borankova K, et al. Serial Xenotransplantation in NSG Mice Promotes a Hybrid Epithelial/Mesenchymal Gene Expression Signature and Stemness in Rhabdomyosarcoma Cells. Cancers (Basel). 2020;12: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7a
Farahani R, Rezaei Lotfi S, Simonian M, Xaymardan M, Hunter N. Neural microvascular pericytes contribute to human adult neurogenesis. J Comp Neurol. 2019;527:780-796 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1g
Wang C, Najm R, Xu Q, Jeong D, Walker D, Balestra M, et al. Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector. Nat Med. 2018;24:647-657 pubmed publisher
  • immunocytochemistry; human; fig 3a
Markmiller S, Soltanieh S, Server K, Mak R, Jin W, Fang M, et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 2018;172:590-604.e13 pubmed publisher
  • immunohistochemistry - frozen section; human; loading ...; fig 3a
Sartore R, Cardoso S, Lages Y, Paraguassu J, Stelling M, Madeiro da Costa R, et al. Trace elements during primordial plexiform network formation in human cerebral organoids. Peerj. 2017;5:e2927 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 4a
Palmero E, Palmero S, Murrell W. Brain tissue banking for stem cells for our future. Sci Rep. 2016;6:39394 pubmed publisher
  • flow cytometry; human; 1:200; loading ...
  • immunocytochemistry; human; 1:200; loading ...; fig s1d
Zhu L, Gómez Durán A, Saretzki G, Jin S, Tilgner K, Melguizo Sanchís D, et al. The mitochondrial protein CHCHD2 primes the differentiation potential of human induced pluripotent stem cells to neuroectodermal lineages. J Cell Biol. 2016;215:187-202 pubmed
  • immunocytochemistry; human; fig 1e
Zhang L, Hua Q, Tang K, Shi C, Xie X, Zhang R. CXCR4 activation promotes differentiation of human embryonic stem cells to neural stem cells. Neuroscience. 2016;337:88-97 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; tbl s4
Hansen S, Stummann T, Borland H, Hasholt L, Tumer Z, Nielsen J, et al. Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3. Stem Cell Res. 2016;17:306-317 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 3
Son M, Lee M, Jeon H, Seol B, Kim J, Chang J, et al. Generation and characterization of integration-free induced pluripotent stem cells from patients with autoimmune disease. Exp Mol Med. 2016;48:e232 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 2
De Filippis L, Halikere A, McGowan H, Moore J, Tischfield J, Hart R, et al. Ethanol-mediated activation of the NLRP3 inflammasome in iPS cells and iPS cells-derived neural progenitor cells. Mol Brain. 2016;9:51 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 5
Douvaras P, Rusielewicz T, Kim K, Haines J, CASACCIA P, Fossati V. Epigenetic Modulation of Human Induced Pluripotent Stem Cell Differentiation to Oligodendrocytes. Int J Mol Sci. 2016;17: pubmed publisher
  • immunocytochemistry; human; fig 4n
Suchanek J, Suchánková Kleplová T, Rehacek V, Browne K, Soukup T. Proliferative Capacity and Phenotypical Alteration of Multipotent Ecto-Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth Cultured in Xenogeneic and Allogeneic Media. Folia Biol (Praha). 2016;62:1-14 pubmed
  • immunocytochemistry; human; fig 1
Rolland M, Li X, Sellier Y, Martin H, Pérez Berezo T, Rauwel B, et al. PPARγ Is Activated during Congenital Cytomegalovirus Infection and Inhibits Neuronogenesis from Human Neural Stem Cells. PLoS Pathog. 2016;12:e1005547 pubmed publisher
  • immunocytochemistry; human; 1:2000; fig 2
Francis K, Ton A, Xin Y, O Halloran P, Wassif C, Malik N, et al. Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes. Nat Med. 2016;22:388-96 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 2
Sancho Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016;7:10743 pubmed publisher
  • immunocytochemistry; human; 1:200; tbl 1
de Souza C, Nivison Smith L, Christie D, Polkinghorne P, McGhee C, Kalloniatis M, et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp Eye Res. 2016;150:135-48 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; tbl 1
Mikhailova A, Ilmarinen T, Ratnayake A, Petrovski G, Uusitalo H, Skottman H, et al. Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction. Exp Eye Res. 2016;146:26-34 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 1
Stebbins M, Wilson H, Canfield S, Qian T, Palecek S, Shusta E. Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells. Methods. 2016;101:93-102 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 17j
Sugiyama S, Yoshino Y, Kuriyama S, Inoue M, Komine K, Otsuka K, et al. A Curcumin Analog, GO-Y078, Effectively Inhibits Angiogenesis through Actin Disorganization. Anticancer Agents Med Chem. 2016;16:633-47 pubmed
  • immunohistochemistry; giant panda; 1:100; fig 4
Prescott H, Manning C, Gardner A, Ritchie W, Pizzi R, Girling S, et al. Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells. PLoS ONE. 2015;10:e0138840 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig s1f
Uchida H, Morita T, Niizuma K, Kushida Y, Kuroda Y, Wakao S, et al. Transplantation of Unique Subpopulation of Fibroblasts, Muse Cells, Ameliorates Experimental Stroke Possibly via Robust Neuronal Differentiation. Stem Cells. 2016;34:160-73 pubmed publisher
  • flow cytometry; human; fig 1
Priest C, Manley N, Denham J, Wirth E, Lebkowski J. Preclinical safety of human embryonic stem cell-derived oligodendrocyte progenitors supporting clinical trials in spinal cord injury. Regen Med. 2015;10:939-58 pubmed publisher
  • western blot; human; 1:1000; fig s8b
Mughal A, Grieg Z, Skjellegrind H, Fayzullin A, Lamkhannat M, Joel M, et al. Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells. Mol Cancer. 2015;14:160 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 2
Ishikawa M, Ohnishi H, Skerleva D, Sakamoto T, Yamamoto N, Hotta A, et al. Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae. J Tissue Eng Regen Med. 2017;11:1766-1778 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 3h
Garcia Calero E, Botella Lopez A, Bahamonde O, Perez Balaguer A, Martinez S. FoxP2 protein levels regulate cell morphology changes and migration patterns in the vertebrate developing telencephalon. Brain Struct Funct. 2016;221:2905-17 pubmed publisher
  • immunocytochemistry; rat; 1:100
Tang N, Lyu D, Liu T, Chen F, Jing S, Hao T, et al. Different Effects of p52SHC1 and p52SHC3 on the Cell Cycle of Neurons and Neural Stem Cells. J Cell Physiol. 2016;231:172-80 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:75; loading ...; fig 1
Zidlik V, Brychtova S, Uvirova M, Ziak D, Dvorackova J. The changes of angiogenesis and immune cell infiltration in the intra- and peri-tumoral melanoma microenvironment. Int J Mol Sci. 2015;16:7876-89 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 2Af
Machado C, Griesi Oliveira K, Rosenberg C, Kok F, Martins S, Passos Bueno M, et al. Collybistin binds and inhibits mTORC1 signaling: a potential novel mechanism contributing to intellectual disability and autism. Eur J Hum Genet. 2016;24:59-65 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 3
  • western blot; human; 1:200; fig 5
Hossini A, Megges M, Prigione A, Lichtner B, Toliat M, Wruck W, et al. Induced pluripotent stem cell-derived neuronal cells from a sporadic Alzheimer's disease donor as a model for investigating AD-associated gene regulatory networks. BMC Genomics. 2015;16:84 pubmed publisher
  • immunocytochemistry; human
Peretz Y, Wu H, Patel S, Bellacosa A, Katz R. Inhibitor of DNA Binding 4 (ID4) is highly expressed in human melanoma tissues and may function to restrict normal differentiation of melanoma cells. PLoS ONE. 2015;10:e0116839 pubmed publisher
  • immunocytochemistry; human
Lankford L, Selby T, Becker J, Ryzhuk V, Long C, Farmer D, et al. Early gestation chorionic villi-derived stromal cells for fetal tissue engineering. World J Stem Cells. 2015;7:195-207 pubmed publisher
  • immunocytochemistry; human; 1:200
Kumar M, Csaba Z, Peineau S, Srivastava R, Rasika S, Mani S, et al. Endogenous cerebellar neurogenesis in adult mice with progressive ataxia. Ann Clin Transl Neurol. 2014;1:968-81 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 1
  • immunocytochemistry; human; 1:200; fig 5
Yao P, Kang D, Wang X, Lin R, Ye Z. Cell-density-dependent manifestation of partial characteristics for neuronal precursors in a newly established human gliosarcoma cell line. In Vitro Cell Dev Biol Anim. 2015;51:345-52 pubmed publisher
  • immunocytochemistry; human; 1:250
  • immunocytochemistry; mouse; 1:250
Wan C, O Carroll S, Kim S, Green C, Nicholson L. Spatiotemporal changes in Cx30 and Cx43 expression during neuronal differentiation of P19 EC and NT2/D1 cells. Cell Biol Int Rep (2010). 2013;20:13-23 pubmed
  • immunocytochemistry; human; 1:100; tbl 1
Johnstone S, Liley M, Dalby M, Barnett S. Comparison of human olfactory and skeletal MSCs using osteogenic nanotopography to demonstrate bone-specific bioactivity of the surfaces. Acta Biomater. 2015;13:266-76 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 2
Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
  • immunocytochemistry; human; 1:400; tbl 1
Gervois P, Struys T, Hilkens P, Bronckaers A, Ratajczak J, Politis C, et al. Neurogenic maturation of human dental pulp stem cells following neurosphere generation induces morphological and electrophysiological characteristics of functional neurons. Stem Cells Dev. 2015;24:296-311 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1b
Lee H, Kim K, Lim H, Choi M, Kim H, Ahn H, et al. Priming Wharton's jelly-derived mesenchymal stromal/stem cells with ROCK inhibitor improves recovery in an intracerebral hemorrhage model. J Cell Biochem. 2015;116:310-9 pubmed publisher
  • western blot; human; 1:1000; fig 3
Van Brocklyn J, Wojton J, Meisen W, Kellough D, Ecsedy J, Kaur B, et al. Aurora-A inhibition offers a novel therapy effective against intracranial glioblastoma. Cancer Res. 2014;74:5364-70 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 7
Liu G, Suzuki K, Li M, Qu J, Montserrat N, Tarantino C, et al. Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs. Nat Commun. 2014;5:4330 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Oh S, Park H, Hwang I, Park H, Choi K, Jeong H, et al. Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons. ScientificWorldJournal. 2014;2014:957548 pubmed publisher
  • immunocytochemistry; human; 1:200
Muchkaeva I, Dashinimaev E, Artyuhov A, Myagkova E, Vorotelyak E, Yegorov Y, et al. Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells. Acta Naturae. 2014;6:45-53 pubmed
  • immunocytochemistry; human
Nguyen H, Nekanti U, Haus D, Funes G, Moreno D, Kamei N, et al. Induction of early neural precursors and derivation of tripotent neural stem cells from human pluripotent stem cells under xeno-free conditions. J Comp Neurol. 2014;522:2767-83 pubmed publisher
  • immunohistochemistry; human
Wang Z, Wang B, Shi Y, Xu C, Xiao H, Ma L, et al. Oncogenic miR-20a and miR-106a enhance the invasiveness of human glioma stem cells by directly targeting TIMP-2. Oncogene. 2015;34:1407-19 pubmed publisher
  • immunocytochemistry; mouse; 1:1 000
Cheng L, Hu W, Qiu B, Zhao J, Yu Y, Guan W, et al. Generation of neural progenitor cells by chemical cocktails and hypoxia. Cell Res. 2014;24:665-79 pubmed publisher
  • immunocytochemistry; human; 1:100
Wyatt L, Filbin M, Keirstead H. PTEN inhibition enhances neurite outgrowth in human embryonic stem cell-derived neuronal progenitor cells. J Comp Neurol. 2014;522:2741-55 pubmed publisher
  • flow cytometry; rhesus macaque; 1:100
Nemati S, Jabbari R, Hajinasrollah M, Zare Mehrjerdi N, Azizi H, Hemmesi K, et al. Transplantation of adult monkey neural stem cells into a contusion spinal cord injury model in rhesus macaque monkeys. Cell J. 2014;16:117-130 pubmed
  • immunocytochemistry; human; 1:1000
Macdonald C, Unsworth C, Graham E. Enrichment of differentiated hNT neurons and subsequent analysis using flow-cytometry and xCELLigence sensing. J Neurosci Methods. 2014;227:47-56 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:500
Lee K, Seo J, Shin J, Ji E, Roh J, Kim J, et al. Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system. Proc Natl Acad Sci U S A. 2014;111:2794-9 pubmed publisher
  • immunohistochemistry - paraffin section; human
Ye J, Vives Pi M, Gillespie K. Maternal microchimerism: increased in the insulin positive compartment of type 1 diabetes pancreas but not in infiltrating immune cells or replicating islet cells. PLoS ONE. 2014;9:e86985 pubmed publisher
  • immunocytochemistry; human; 1:100
Mallon B, Hamilton R, Kozhich O, Johnson K, Fann Y, Rao M, et al. Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin. Stem Cell Res. 2014;12:376-86 pubmed publisher
  • immunocytochemistry; human
Zhou J, Lu P, Ren H, Zheng Z, Ji J, Liu H, et al. 17?-estradiol protects human eyelid-derived adipose stem cells against cytotoxicity and increases transplanted cell survival in spinal cord injury. J Cell Mol Med. 2014;18:326-43 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000
Larson A, Lee C, Lezcano C, Zhan Q, Huang J, Fischer A, et al. Melanoma spheroid formation involves laminin-associated vasculogenic mimicry. Am J Pathol. 2014;184:71-8 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1600; fig s1
Jelen S, Parm Ulhøi B, Larsen A, Frøkiær J, Nielsen S, Rutzler M. AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15(+)/CalB(+) leukocytes. PLoS ONE. 2013;8:e75764 pubmed publisher
  • immunocytochemistry; pigs ; 1:200
Lim J, McCullen S, Piedrahita J, Loboa E, Olby N. Alternating current electric fields of varying frequencies: effects on proliferation and differentiation of porcine neural progenitor cells. Cell Reprogram. 2013;15:405-12 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500
  • western blot; human; 1:2000
Mao X, Hütt Cabezas M, Orr B, Weingart M, Taylor I, Rajan A, et al. LIN28A facilitates the transformation of human neural stem cells and promotes glioblastoma tumorigenesis through a pro-invasive genetic program. Oncotarget. 2013;4:1050-64 pubmed
  • immunocytochemistry; human; 1:100
Hou P, Chuang C, Kao C, Chou S, Stone L, Ho H, et al. LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1. Nucleic Acids Res. 2013;41:7753-70 pubmed publisher
  • immunohistochemistry; human; 1:200
  • western blot; human; 1:1000
Zemp F, Lun X, McKenzie B, Zhou H, Maxwell L, Sun B, et al. Treating brain tumor-initiating cells using a combination of myxoma virus and rapamycin. Neuro Oncol. 2013;15:904-20 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
Shahhoseini M, Taghizadeh Z, Hatami M, Baharvand H. Retinoic acid dependent histone 3 demethylation of the clustered HOX genes during neural differentiation of human embryonic stem cells. Biochem Cell Biol. 2013;91:116-22 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200
Prabowo A, Anink J, Lammens M, Nellist M, van den Ouweland A, Adle Biassette H, et al. Fetal brain lesions in tuberous sclerosis complex: TORC1 activation and inflammation. Brain Pathol. 2013;23:45-59 pubmed publisher
  • immunohistochemistry; human
Garzon Muvdi T, Schiapparelli P, Ap Rhys C, Guerrero Cazares H, Smith C, Kim D, et al. Regulation of brain tumor dispersal by NKCC1 through a novel role in focal adhesion regulation. PLoS Biol. 2012;10:e1001320 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50, 1:100, 1:200
Sellheyer K, Krahl D. Spatiotemporal expression pattern of neuroepithelial stem cell marker nestin suggests a role in dermal homeostasis, neovasculogenesis, and tumor stroma development: a study on embryonic and adult human skin. J Am Acad Dermatol. 2010;63:93-113 pubmed publisher
  • immunocytochemistry; human; 1:1000
Leonard B, Mastroeni D, Grover A, Liu Q, Yang K, Gao M, et al. Subventricular zone neural progenitors from rapid brain autopsies of elderly subjects with and without neurodegenerative disease. J Comp Neurol. 2009;515:269-94 pubmed publisher
Matsumura K, Seiriki K, Okada S, Nagase M, Ayabe S, Yamada I, et al. Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes. Nat Commun. 2020;11:859 pubmed publisher
Chen J, Ananthanarayanan B, Springer K, Wolf K, Sheyman S, Tran V, et al. Suppression of LIM Kinase 1 and LIM Kinase 2 Limits Glioblastoma Invasion. Cancer Res. 2020;80:69-78 pubmed publisher
Boso D, Rampazzo E, Zanon C, Bresolin S, Maule F, Porcù E, et al. HIF-1α/Wnt signaling-dependent control of gene transcription regulates neuronal differentiation of glioblastoma stem cells. Theranostics. 2019;9:4860-4877 pubmed publisher
Terada Y, Jo N, Arakawa Y, Sakakura M, Yamada Y, Ukai T, et al. Human Pluripotent Stem Cell-Derived Tumor Model Uncovers the Embryonic Stem Cell Signature as a Key Driver in Atypical Teratoid/Rhabdoid Tumor. Cell Rep. 2019;26:2608-2621.e6 pubmed publisher
Jeon H, Kim J, Choi J, Han E, Song C, Lee J, et al. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing. J Clin Med. 2018;7: pubmed publisher
Kim E, Hwang S, Yoon J, Kim H, Lee G, Hyun S. Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic piglet. BMC Vet Res. 2018;14:331 pubmed publisher
Omer Javed A, Li Y, Muffat J, Su K, Cohen M, Lungjangwa T, et al. Microcephaly Modeling of Kinetochore Mutation Reveals a Brain-Specific Phenotype. Cell Rep. 2018;25:368-382.e5 pubmed publisher
Sunamura N, Iwashita S, Enomoto K, Kadoshima T, Isono F. Loss of the fragile X mental retardation protein causes aberrant differentiation in human neural progenitor cells. Sci Rep. 2018;8:11585 pubmed publisher
Vetreno R, Lawrimore C, ROWSEY P, Crews F. Persistent Adult Neuroimmune Activation and Loss of Hippocampal Neurogenesis Following Adolescent Ethanol Exposure: Blockade by Exercise and the Anti-inflammatory Drug Indomethacin. Front Neurosci. 2018;12:200 pubmed publisher
Caja L, Tzavlaki K, Dadras M, Tan E, Hatem G, Maturi N, et al. Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells. Oncogene. 2018;37:2515-2531 pubmed publisher
Scior A, Buntru A, Arnsburg K, Ast A, Iburg M, Juenemann K, et al. Complete suppression of Htt fibrilization and disaggregation of Htt fibrils by a trimeric chaperone complex. EMBO J. 2018;37:282-299 pubmed publisher
Lan X, Jörg D, Cavalli F, Richards L, Nguyen L, Vanner R, et al. Fate mapping of human glioblastoma reveals an invariant stem cell hierarchy. Nature. 2017;549:227-232 pubmed publisher
Kraft A, Jubal E, von Laer R, Döring C, Rocha A, Grebbin M, et al. Astrocytic Calcium Waves Signal Brain Injury to Neural Stem and Progenitor Cells. Stem Cell Reports. 2017;8:701-714 pubmed publisher
Hanaford A, Archer T, Price A, Kahlert U, Maciaczyk J, Nikkhah G, et al. DiSCoVERing Innovative Therapies for Rare Tumors: Combining Genetically Accurate Disease Models with In Silico Analysis to Identify Novel Therapeutic Targets. Clin Cancer Res. 2016;22:3903-14 pubmed publisher
Fu L, Xu X, Ren R, Wu J, Zhang W, Yang J, et al. Modeling xeroderma pigmentosum associated neurological pathologies with patients-derived iPSCs. Protein Cell. 2016;7:210-21 pubmed publisher
Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, et al. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nat Commun. 2015;6:10068 pubmed publisher
Balasubramaniyan V, Vaillant B, Wang S, Gumin J, Butalid M, Sai K, et al. Aberrant mesenchymal differentiation of glioma stem-like cells: implications for therapeutic targeting. Oncotarget. 2015;6:31007-17 pubmed publisher
Zhou H, Martínez H, Sun B, Li A, Zimmer M, Katsanis N, et al. Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood. Stem Cell Rev. 2015;11:652-65 pubmed publisher
Chow C, Guo W, Trivedi P, Zhao X, Gubbels S. Characterization of a unique cell population marked by transgene expression in the adult cochlea of nestin-CreER(T2)/tdTomato-reporter mice. J Comp Neurol. 2015;523:1474-87 pubmed publisher
Feng Y, Zhu M, Dangelmajer S, Lee Y, Wijesekera O, Castellanos C, et al. Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer. Cell Death Dis. 2014;5:e1567 pubmed publisher
Ooi J, Hayden M, Pouladi M. Inhibition of Excessive Monoamine Oxidase A/B Activity Protects Against Stress-induced Neuronal Death in Huntington Disease. Mol Neurobiol. 2015;52:1850-1861 pubmed publisher
Lee C, Zhan Q, Lezcano C, Frank M, Huang J, Larson A, et al. Nestin depletion induces melanoma matrix metalloproteinases and invasion. Lab Invest. 2014;94:1382-95 pubmed publisher
Leite C, Silva N, Mendes S, Ribeiro A, de Faria J, Lourenço T, et al. Differentiation of human umbilical cord matrix mesenchymal stem cells into neural-like progenitor cells and maturation into an oligodendroglial-like lineage. PLoS ONE. 2014;9:e111059 pubmed publisher
Fack F, Espedal H, Keunen O, Golebiewska A, Obad N, Harter P, et al. Bevacizumab treatment induces metabolic adaptation toward anaerobic metabolism in glioblastomas. Acta Neuropathol. 2015;129:115-31 pubmed publisher
Qendro V, Lundgren D, Rezaul K, Mahony F, Ferrell N, Bi A, et al. Large-scale proteomic characterization of melanoma expressed proteins reveals nestin and vimentin as biomarkers that can potentially distinguish melanoma subtypes. J Proteome Res. 2014;13:5031-40 pubmed publisher
Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling. J Neurosci. 2014;34:12788-800 pubmed publisher
Seino M, Okada M, Shibuya K, Seino S, Suzuki S, Ohta T, et al. Requirement of JNK signaling for self-renewal and tumor-initiating capacity of ovarian cancer stem cells. Anticancer Res. 2014;34:4723-31 pubmed
Choschzick I, Hirseland E, Cramer H, Schultz S, Leppert J, Tronnier V, et al. Responsiveness of stem-like human glioma cells to all-trans retinoic acid and requirement of retinoic acid receptor isotypes α, β and γ. Neuroscience. 2014;279:44-64 pubmed publisher
Kawai H, Satomi K, Morishita Y, Murata Y, Sugano M, Nakano N, et al. Developmental markers of ganglion cells in the enteric nervous system and their application for evaluation of Hirschsprung disease. Pathol Int. 2014;64:432-42 pubmed publisher
Baker G, Yadav V, Motsch S, Koschmann C, Calinescu A, Mineharu Y, et al. Mechanisms of glioma formation: iterative perivascular glioma growth and invasion leads to tumor progression, VEGF-independent vascularization, and resistance to antiangiogenic therapy. Neoplasia. 2014;16:543-61 pubmed publisher
Cortes C, Miranda H, Frankowski H, Batlevi Y, Young J, Le A, et al. Polyglutamine-expanded androgen receptor interferes with TFEB to elicit autophagy defects in SBMA. Nat Neurosci. 2014;17:1180-9 pubmed publisher
Na J, Kim H, Jung J, Kim Y, Kim S, Lee J, et al. Granular cell tumours of the colorectum: histopathological and immunohistochemical evaluation of 30 cases. Histopathology. 2014;65:764-74 pubmed publisher
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product information
Catalog Number :
MAB5326
Subcategory :
Stem Cell Research
Product Name :
Anti-Nestin Antibody, clone 10C2
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P48681
Host Name :
Mouse
Antigen :
Nestin
Clone :
10C2
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Nestin Antibody, clone 10C2
Cross Reactivity :
Human
Background :
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Immunogen :
Fusion protein
Specificity :
Reacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin.
Package Size :
100 μg
Uses :
Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Antibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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