This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Tyrosine Hydroxylase Antibody, clone 2/40/15
catalog :
MAB5280
quantity :
40 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2/40/15
reactivity :
Shaw's jird, tree shrew, human, mouse, rat, bovine, domestic sheep, rhesus macaque
application :
western blot, immunohistochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 76
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - free floating section; human; 1:6000; loading ...; fig 4c
  • immunohistochemistry - free floating section; tree shrew; 1:6000; loading ...; fig 4a
  • immunohistochemistry - frozen section; tree shrew; 1:6000; loading ...; fig 3
  • western blot; tree shrew; 1:20,000; loading ...; fig 1a
  • immunohistochemistry - free floating section; rat; 1:6000; loading ...; fig 4b
Huang Z, Ni R, Luo P, Zhou J. Distribution of tyrosine-hydroxylase-immunoreactive neurons in the hypothalamus of tree shrews. J Comp Neurol. 2019;: pubmed publisher
  • immunohistochemistry; Shaw's jird; 1:250; loading ...; tbl 1
Hammoum I, Benlarbi M, Dellaa A, Szabó K, Dékány B, Csaba D, et al. Study of retinal neurodegeneration and maculopathy in diabetic Meriones shawi: A particular animal model with human-like macula. J Comp Neurol. 2017;525:2890-2914 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:1000; loading ...; fig 1a
Beloate L, Omrani A, Adan R, Webb I, Coolen L. Ventral Tegmental Area Dopamine Cell Activation during Male Rat Sexual Behavior Regulates Neuroplasticity and d-Amphetamine Cross-Sensitization following Sex Abstinence. J Neurosci. 2016;36:9949-61 pubmed publisher
  • immunohistochemistry; domestic sheep; 1:10,000
Grachev P, Porter K, Coolen L, McCosh R, Connors J, Hileman S, et al. Surge-Like Luteinising Hormone Secretion Induced by Retrochiasmatic Area NK3R Activation is Mediated Primarily by Arcuate Kisspeptin Neurones in the Ewe. J Neuroendocrinol. 2016;28: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 4.f,g
Watanabe S, Sanuki R, Sugita Y, Imai W, Yamazaki R, Kozuka T, et al. Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity. J Neurosci. 2015;35:8004-20 pubmed publisher
  • immunohistochemistry - free floating section; rhesus macaque; 1:1000
Weltzien F, Percival K, Martin P, Grünert U. Analysis of bipolar and amacrine populations in marmoset retina. J Comp Neurol. 2015;523:313-34 pubmed publisher
  • immunohistochemistry - free floating section; rat; 1:2000
Guimarães P, Huber D, Campagnole Santos M, Schreihofer A. Development of attenuated baroreflexes in obese Zucker rats coincides with impaired activation of nucleus tractus solitarius. Am J Physiol Regul Integr Comp Physiol. 2014;306:R681-92 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:2000
Piskuric N, Vollmer C, Nurse C. Confocal immunofluorescence study of rat aortic body chemoreceptors and associated neurons in situ and in vitro. J Comp Neurol. 2011;519:856-73 pubmed publisher
  • immunohistochemistry - frozen section; rat
Hernandez Montiel H, Tamariz E, Sandoval Minero M, Varela Echavarria A. Semaphorins 3A, 3C, and 3F in mesencephalic dopaminergic axon pathfinding. J Comp Neurol. 2008;506:387-97 pubmed
Csikós V, Varro P, Bódi V, Oláh S, Vilagi I, Dobolyi A. The mycotoxin deoxynivalenol activates GABAergic neurons in the reward system and inhibits feeding and maternal behaviours. Arch Toxicol. 2020;94:3297-3313 pubmed publisher
Lopes P, Campos A, Fonoff E, Britto L, Pagano R. Motor cortex and pain control: exploring the descending relay analgesic pathways and spinal nociceptive neurons in healthy conscious rats. Behav Brain Funct. 2019;15:5 pubmed publisher
Bragado Alonso S, Reinert J, Marichal N, Massalini S, Berninger B, Kuner T, et al. An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants. EMBO J. 2019;38: pubmed publisher
Franco Iborra S, Cuadros T, Parent A, Romero Gimenez J, Vila M, Perier C. Defective mitochondrial protein import contributes to complex I-induced mitochondrial dysfunction and neurodegeneration in Parkinson's disease. Cell Death Dis. 2018;9:1122 pubmed publisher
Playne R, Jones K, Connor B. Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors. J Stem Cells Regen Med. 2018;14:34-44 pubmed
Pahng A, Paulsen R, McGinn M, Edwards K, Edwards S. Neurobiological Correlates of Pain Avoidance-Like Behavior in Morphine-Dependent and Non-Dependent Rats. Neuroscience. 2017;366:1-14 pubmed publisher
Zapiec B, Dieriks B, Tan S, Faull R, Mombaerts P, Curtis M. A ventral glomerular deficit in Parkinson's disease revealed by whole olfactory bulb reconstruction. Brain. 2017;140:2722-2736 pubmed publisher
Favier M, Carcenac C, Drui G, Vachez Y, Boulet S, Savasta M, et al. Implication of dorsostriatal D3 receptors in motivational processes: a potential target for neuropsychiatric symptoms in Parkinson's disease. Sci Rep. 2017;7:41589 pubmed publisher
Mravec B, Lejavova K, Vargovic P, Ondicova K, Horvathova L, Novak P, et al. Tauopathy in transgenic (SHR72) rats impairs function of central noradrenergic system and promotes neuroinflammation. J Neuroinflammation. 2016;13:15 pubmed publisher
Fernández Santiago R, Carballo Carbajal I, Castellano G, Torrent R, Richaud Y, Sánchez Danés A, et al. Aberrant epigenome in iPSC-derived dopaminergic neurons from Parkinson's disease patients. EMBO Mol Med. 2015;7:1529-46 pubmed publisher
Wang L, Mogami S, Yakabi S, Karasawa H, Yamada C, Yakabi K, et al. Patterns of Brain Activation and Meal Reduction Induced by Abdominal Surgery in Mice and Modulation by Rikkunshito. PLoS ONE. 2015;10:e0139325 pubmed publisher
Hall Z, Healy S, Meddle S. A role for nonapeptides and dopamine in nest-building behaviour. J Neuroendocrinol. 2015;27:158-65 pubmed publisher
Tando S, Itoh K, Yaoi T, Ogi H, Goto S, Mori M, et al. Bisphenol A exposure disrupts the development of the locus coeruleus-noradrenergic system in mice. Neuropathology. 2014;34:527-34 pubmed publisher
Tatti R, Bhaukaurally K, Gschwend O, Seal R, Edwards R, Rodriguez I, et al. A population of glomerular glutamatergic neurons controls sensory information transfer in the mouse olfactory bulb. Nat Commun. 2014;5:3791 pubmed publisher
McCollum L, Roberts R. Ultrastructural localization of tyrosine hydroxylase in tree shrew nucleus accumbens core and shell. Neuroscience. 2014;271:23-34 pubmed publisher
Wagner F, Stroh T, Veh R. Correlating habenular subnuclei in rat and mouse by using topographic, morphological, and cytochemical criteria. J Comp Neurol. 2014;522:2650-62 pubmed publisher
Piskuric N, Zhang M, Vollmer C, Nurse C. Potential roles of ATP and local neurons in the monitoring of blood O2 content by rat aortic bodies. Exp Physiol. 2014;99:248-61 pubmed publisher
Ballaz S, Morales I, Rodriguez M, Obeso J. Ascorbate prevents cell death from prolonged exposure to glutamate in an in vitro model of human dopaminergic neurons. J Neurosci Res. 2013;91:1609-17 pubmed publisher
Khalil O, Forrest C, Pisar M, Smith R, Darlington L, Stone T. Prenatal activation of maternal TLR3 receptors by viral-mimetic poly(I:C) modifies GluN2B expression in embryos and sonic hedgehog in offspring in the absence of kynurenine pathway activation. Immunopharmacol Immunotoxicol. 2013;35:581-93 pubmed publisher
Weltzien F, Dimarco S, Protti D, Daraio T, Martin P, Grünert U. Characterization of secretagogin-immunoreactive amacrine cells in marmoset retina. J Comp Neurol. 2014;522:435-55 pubmed publisher
Alvarez Fischer D, Noelker C, Grünewald A, Vulinović F, Guerreiro S, Fuchs J, et al. Probenecid potentiates MPTP/MPP+ toxicity by interference with cellular energy metabolism. J Neurochem. 2013;127:782-92 pubmed publisher
Chocyk A, Bobula B, Dudys D, Przyborowska A, Majcher Maślanka I, Hess G, et al. Early-life stress affects the structural and functional plasticity of the medial prefrontal cortex in adolescent rats. Eur J Neurosci. 2013;38:2089-107 pubmed publisher
Drui G, Carnicella S, Carcenac C, Favier M, Bertrand A, Boulet S, et al. Loss of dopaminergic nigrostriatal neurons accounts for the motivational and affective deficits in Parkinson's disease. Mol Psychiatry. 2014;19:358-67 pubmed publisher
Vargovic P, Ukropec J, Laukova M, Kurdiova T, Balaz M, Manz B, et al. Repeated immobilization stress induces catecholamine production in rat mesenteric adipocytes. Stress. 2013;16:340-52 pubmed publisher
Rojas P, Ruiz Sanchez E, Rojas C, Ogren S. Ginkgo biloba extract (EGb 761) modulates the expression of dopamine-related genes in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinsonism in mice. Neuroscience. 2012;223:246-57 pubmed publisher
Lu Y, Simpson K, Weaver K, Lin R. Differential distribution patterns from medial prefrontal cortex and dorsal raphe to the locus coeruleus in rats. Anat Rec (Hoboken). 2012;295:1192-201 pubmed publisher
Jhou T, Xu S, Lee M, Gallen C, Ikemoto S. Mapping of reinforcing and analgesic effects of the mu opioid agonist endomorphin-1 in the ventral midbrain of the rat. Psychopharmacology (Berl). 2012;224:303-12 pubmed publisher
Melendez Ferro M, Rice M, Roberts R, Perez Costas E. Dual use of immunohistochemistry for film densitometry and light microscopy. J Neurosci Methods. 2012;208:86-91 pubmed publisher
Perez Costas E, Melendez Ferro M, Rice M, Conley R, Roberts R. Dopamine pathology in schizophrenia: analysis of total and phosphorylated tyrosine hydroxylase in the substantia nigra. Front Psychiatry. 2012;3:31 pubmed publisher
Sánchez Danés A, Richaud Patin Y, Carballo Carbajal I, Jimenez Delgado S, Caig C, Mora S, et al. Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease. EMBO Mol Med. 2012;4:380-95 pubmed publisher
Kvetnansky R, Ukropec J, Laukova M, Manz B, Pacak K, Vargovic P. Stress stimulates production of catecholamines in rat adipocytes. Cell Mol Neurobiol. 2012;32:801-13 pubmed publisher
Blesa J, Juri C, García Cabezas M, Adánez R, Sánchez González M, Cavada C, et al. Inter-hemispheric asymmetry of nigrostriatal dopaminergic lesion: a possible compensatory mechanism in Parkinson's disease. Front Syst Neurosci. 2011;5:92 pubmed publisher
Chung K, Freestone P, Lipski J. Expression and functional properties of TRPM2 channels in dopaminergic neurons of the substantia nigra of the rat. J Neurophysiol. 2011;106:2865-75 pubmed publisher
Rice M, Roberts R, Melendez Ferro M, Perez Costas E. Neurochemical characterization of the tree shrew dorsal striatum. Front Neuroanat. 2011;5:53 pubmed publisher
Ratzka A, Baron O, Grothe C. FGF-2 deficiency does not influence FGF ligand and receptor expression during development of the nigrostriatal system. PLoS ONE. 2011;6:e23564 pubmed publisher
Tan W, Sherman D, Turesson J, Shao X, Janczewski W, Feldman J. Reelin demarcates a subset of pre-Bötzinger complex neurons in adult rat. J Comp Neurol. 2012;520:606-19 pubmed publisher
Vargovic P, Ukropec J, Laukova M, Cleary S, Manz B, Pacak K, et al. Adipocytes as a new source of catecholamine production. FEBS Lett. 2011;585:2279-84 pubmed publisher
Ventura A, Kusakabe M, Takei Y. Salinity-dependent in vitro effects of homologous natriuretic peptides on the pituitary-interrenal axis in eels. Gen Comp Endocrinol. 2011;173:129-38 pubmed publisher
Billig G, Pál B, Fidzinski P, Jentsch T. Ca2+-activated Cl? currents are dispensable for olfaction. Nat Neurosci. 2011;14:763-9 pubmed publisher
Gackiere F, Saliba L, Baude A, Bosler O, Strube C. Ozone inhalation activates stress-responsive regions of the CNS. J Neurochem. 2011;117:961-72 pubmed publisher
Chocyk A, Przyborowska A, Dudys D, Majcher I, Mackowiak M, Wedzony K. The impact of maternal separation on the number of tyrosine hydroxylase-expressing midbrain neurons during different stages of ontogenesis. Neuroscience. 2011;182:43-61 pubmed publisher
Nullmeier S, Panther P, Dobrowolny H, Frotscher M, Zhao S, Schwegler H, et al. Region-specific alteration of GABAergic markers in the brain of heterozygous reeler mice. Eur J Neurosci. 2011;33:689-98 pubmed publisher
Holstein G, Friedrich V, Kang T, Kukielka E, Martinelli G. Direct projections from the caudal vestibular nuclei to the ventrolateral medulla in the rat. Neuroscience. 2011;175:104-17 pubmed publisher
Chocyk A, Dudys D, Przyborowska A, Majcher I, Mackowiak M, Wedzony K. Maternal separation affects the number, proliferation and apoptosis of glia cells in the substantia nigra and ventral tegmental area of juvenile rats. Neuroscience. 2011;173:1-18 pubmed publisher
Robertson S, Matthies H, Owens W, Sathananthan V, Christianson N, Kennedy J, et al. Insulin reveals Akt signaling as a novel regulator of norepinephrine transporter trafficking and norepinephrine homeostasis. J Neurosci. 2010;30:11305-16 pubmed publisher
Bachis A, Cruz M, Mocchetti I. M-tropic HIV envelope protein gp120 exhibits a different neuropathological profile than T-tropic gp120 in rat striatum. Eur J Neurosci. 2010;32:570-8 pubmed publisher
Renner E, Szabó Meltzer K, Puskas N, Toth Z, Dobolyi A, Palkovits M. Activation of neurons in the hypothalamic dorsomedial nucleus via hypothalamic projections of the nucleus of the solitary tract following refeeding of fasted rats. Eur J Neurosci. 2010;31:302-14 pubmed publisher
Adori C, Zelena D, Timár J, Gyarmati Z, Domokos A, Sobor M, et al. Intermittent prenatal MDMA exposure alters physiological but not mood related parameters in adult rat offspring. Behav Brain Res. 2010;206:299-309 pubmed publisher
Cobacho N, Serano A, Casarejos M, Mena M, Paíno C. Use of transduced adipose tissue stromal cells as biologic minipumps to deliver levodopa for the treatment of neuropathic pain: possibilities and limitations. Cell Transplant. 2009;18:1341-58 pubmed publisher
Ries V, Cheng H, Baohan A, Kareva T, Oo T, Rzhetskaya M, et al. Regulation of the postnatal development of dopamine neurons of the substantia nigra in vivo by Akt/protein kinase B. J Neurochem. 2009;110:23-33 pubmed publisher
Oo T, Marchionini D, Yarygina O, O Leary P, Hughes R, Kholodilov N, et al. Brain-derived neurotrophic factor regulates early postnatal developmental cell death of dopamine neurons of the substantia nigra in vivo. Mol Cell Neurosci. 2009;41:440-7 pubmed publisher
Garrido Gil P, Belzunegui S, San Sebastián W, Izal Azcárate A, López B, Marcilla I, et al. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine exposure fails to produce delayed degeneration of substantia nigra neurons in monkeys. J Neurosci Res. 2009;87:586-97 pubmed publisher
Varga T, Palkovits M, Usdin T, Dobolyi A. The medial paralemniscal nucleus and its afferent neuronal connections in rat. J Comp Neurol. 2008;511:221-37 pubmed publisher
Brill M, Snapyan M, Wohlfrom H, Ninkovic J, Jawerka M, Mastick G, et al. A dlx2- and pax6-dependent transcriptional code for periglomerular neuron specification in the adult olfactory bulb. J Neurosci. 2008;28:6439-52 pubmed publisher
Lai Y, Hsieh K, Nguyen D, Peever J, Siegel J. Neurotoxic lesions at the ventral mesopontine junction change sleep time and muscle activity during sleep: an animal model of motor disorders in sleep. Neuroscience. 2008;154:431-43 pubmed publisher
Le Brun I, Dufour A, Crest M, Szabo G, Erdelyi F, Baude A. Differential expression of Nk1 and NK3 neurokinin receptors in neurons of the nucleus tractus solitarius and the dorsal vagal motor nucleus of the rat and mouse. Neuroscience. 2008;152:56-64 pubmed publisher
Gruber C, Kahl A, Lebenheim L, Kowski A, Dittgen A, Veh R. Dopaminergic projections from the VTA substantially contribute to the mesohabenular pathway in the rat. Neurosci Lett. 2007;427:165-70 pubmed
Kumar U. Colocalization of somatostatin receptor subtypes (SSTR1-5) with somatostatin, NADPH-diaphorase (NADPH-d), and tyrosine hydroxylase in the rat hypothalamus. J Comp Neurol. 2007;504:185-205 pubmed
Cyr M, Sotnikova T, Gainetdinov R, Caron M. Dopamine enhances motor and neuropathological consequences of polyglutamine expanded huntingtin. FASEB J. 2006;20:2541-3 pubmed
Salahpour A, Medvedev I, Beaulieu J, Gainetdinov R, Caron M. Local knockdown of genes in the brain using small interfering RNA: a phenotypic comparison with knockout animals. Biol Psychiatry. 2007;61:65-9 pubmed
Casini G, Rickman D, Brecha N. Expression of the gamma-aminobutyric acid (GABA) plasma membrane transporter-1 in monkey and human retina. Invest Ophthalmol Vis Sci. 2006;47:1682-90 pubmed
Fedorov V, Hucker W, Dobrzynski H, Rosenshtraukh L, Efimov I. Postganglionic nerve stimulation induces temporal inhibition of excitability in rabbit sinoatrial node. Am J Physiol Heart Circ Physiol. 2006;291:H612-23 pubmed
Yavich L, Oksman M, Tanila H, Kerokoski P, Hiltunen M, van Groen T, et al. Locomotor activity and evoked dopamine release are reduced in mice overexpressing A30P-mutated human alpha-synuclein. Neurobiol Dis. 2005;20:303-13 pubmed
Zvarova K, Vizzard M. Distribution and fate of cocaine- and amphetamine-regulated transcript peptide (CARTp)-expressing cells in rat urinary bladder: a developmental study. J Comp Neurol. 2005;489:501-17 pubmed
Sánchez González M, Garcia Cabezas M, Rico B, Cavada C. The primate thalamus is a key target for brain dopamine. J Neurosci. 2005;25:6076-83 pubmed
Mourlevat S, Debeir T, Ferrario J, Delbe J, Caruelle D, Lejeune O, et al. Pleiotrophin mediates the neurotrophic effect of cyclic AMP on dopaminergic neurons: analysis of suppression-subtracted cDNA libraries and confirmation in vitro. Exp Neurol. 2005;194:243-54 pubmed
Oo T, Ries V, Cho J, Kholodilov N, Burke R. Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat. J Comp Neurol. 2005;484:57-67 pubmed
product information
Catalog Number :
MAB5280
Subcategory :
Neuroscience
Product Name :
Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P07101
Host Name :
Mouse
Antigen :
Tyrosine Hydroxylase
Clone :
2/40/15
Conjugate :
Purified
Isotype :
IgG2a
Product Description :
Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15
Cross Reactivity :
Bovine;Chicken;Quail;Rat
Background :
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
ALT Names :
TH; Tyrosine Monooxygenase
Immunogen :
Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).
Specificity :
Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.
Package Size :
40 µg
Uses :
Immunohistochemistry;Western Blotting
Storage :
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
  • • Cancer
  • • Nuclear Signaling
  • • Apoptosis
  • • Cardiovascular Disease
  • • Developmental Biology
  • • Metabolic Disease
  • • Neurodegenerative Disease
  • • Cell Biology
View all the new antibodies at www.emdmillipore.com.