Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8 | EMD Millipore MAB5278 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8

catalog :

MAB5278

quantity :

500 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PH8

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human	Kao T, Lee H, Higuchi A, Ling Q, Yu W, Chou Y, et al. Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments. J Biomed Mater Res B Appl Biomater. 2014;102:463-76 pubmed publisher
immunohistochemistry - frozen section; rat; 1:4000	Geerling J, Shin J, Chimenti P, Loewy A. Paraventricular hypothalamic nucleus: axonal projections to the brainstem. J Comp Neurol. 2010;518:1460-99 pubmed publisher
	Miller R, Loewy A. 5-HT neurons of the area postrema become c-Fos-activated after increases in plasma sodium levels and transmit interoceptive information to the nucleus accumbens. Am J Physiol Regul Integr Comp Physiol. 2014;306:R663-73 pubmed publisher
	Jaffe E, Stith L, Lawrence S, Andrake M, Dunbrack R. A new model for allosteric regulation of phenylalanine hydroxylase: implications for disease and therapeutics. Arch Biochem Biophys. 2013;530:73-82 pubmed publisher
	Lu Y, Simpson K, Weaver K, Lin R. Coexpression of serotonin and nitric oxide in the raphe complex: cortical versus subcortical circuit. Anat Rec (Hoboken). 2010;293:1954-65 pubmed publisher
	Broadbelt K, Paterson D, Rivera K, Trachtenberg F, Kinney H. Neuroanatomic relationships between the GABAergic and serotonergic systems in the developing human medulla. Auton Neurosci. 2010;154:30-41 pubmed publisher
	Schreyer S, Buttner Ennever J, Tang X, Mustari M, Horn A. Orexin-A inputs onto visuomotor cell groups in the monkey brainstem. Neuroscience. 2009;164:629-40 pubmed publisher
	Paterson D, Darnall R. 5-HT2A receptors are concentrated in regions of the human infant medulla involved in respiratory and autonomic control. Auton Neurosci. 2009;147:48-55 pubmed publisher
	Duncan J, Paterson D, Kinney H. The development of nicotinic receptors in the human medulla oblongata: inter-relationship with the serotonergic system. Auton Neurosci. 2008;144:61-75 pubmed publisher
	Shin J, Geerling J, Loewy A. Inputs to the ventrolateral bed nucleus of the stria terminalis. J Comp Neurol. 2008;511:628-57 pubmed publisher
	Winge I, McKinney J, Ying M, D Santos C, Kleppe R, Knappskog P, et al. Activation and stabilization of human tryptophan hydroxylase 2 by phosphorylation and 14-3-3 binding. Biochem J. 2008;410:195-204 pubmed
	Chen S, Brown I. Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases. Cell Stress Chaperones. 2007;12:51-8 pubmed
	Lebrand C, Gaspar P, Nicolas D, Hornung J. Transitory uptake of serotonin in the developing sensory pathways of the common marmoset. J Comp Neurol. 2006;499:677-89 pubmed
	Tóth I, Tóth D, Boldogkoi Z, Hornyák A, Palkovits M, Blessing W. Serotonin-synthesizing neurons in the rostral medullary raphé/parapyramidal region transneuronally labelled after injection of pseudorabies virus into the rat tail. Neurochem Res. 2006;31:277-86 pubmed

product information

Catalog Number :

MAB5278

Subcategory :

Neuroscience

Product Name :

Anti-Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P17752;P07101

Host Name :

Mouse

Antigen :

Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase

Clone :

PH8

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8

Cross Reactivity :

Human

Background :

PH8 Antibody is a murine monoclonal antibody that binds a common epitope of Tryptophan hydroxylase (TRH), Tyrosine hydroxylase (TYH) and Phenylalanine hydroxylase (PAH).1 In the literature this monoclonal antibody is cited as PH8. Tyrosine hydroxylase (TYH) is the enzyme which converts tyrosine to dihydroxyphenylalanine (L-dopa), a precursor of the catecholamine neurotransmitters dopamine, noradrenaline and adrenaline. Tryptophan hydroxylase (TRH) is the enzyme that converts 5-hydroxytryptophan to serotonin. In fresh tissue the PH8 Antibody binds to TYH and TRH so is a marker for TYH-containing catecholaminergic neurons,2 and serotonergic neurons.3,4,5 Tryptophan hydroxylase (TRH) can be used as a marker for serotonin as it converts 5-hydroxy-tryptophan to serotonin. Serotonin is rapidly metabolised and is unable to be detected by anti-serotonin antibodies in post mortem tissue. In human tissue that has been formalin-fixed, due to a change in the antigenic determinant of tyrosine hydroxylase (TYH), PH8 Antibody will bind only tryptophan hydroxylase (TRH).3,4,5 The PH8 Antibody can therefore be used to specifically identify serotonergic neurons in fixed human tissue. Phenylalanine hydroxylase (PAH) can be detected in hepatic tissue sections using the PH8 Antibody.

Package Size :

500 µg

Uses :

Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

The PH8 Antibody is shipped liquid at ambient temperature. Liquid material is stable for at up to 6 months when stored at 2-8°C.