Neurofilament 200 kDa Antibody, clone N52 | EMD Millipore MAB5266 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Neurofilament 200 kDa Antibody, clone N52

catalog :

MAB5266

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N52

The same clone is also sold as:

Abcam: ab82259
MilliporeSigma: N0142
Boster: MA1071

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; dogs; 1:200; loading ...; fig 8	Badiei A, Sudharsan R, Santana E, Dunaief J, Aguirre G. Comparative localization of cystathionine beta synthases and cystathionine gamma lyase in canine, non-human primate and human retina. Exp Eye Res. 2019;181:72-84 pubmed publisher
immunocytochemistry; rat; 1:800; fig 6a	Kobayashi M, Chandrasekhar A, Cheng C, Martinez J, Ng H, de La Hoz C, et al. Diabetic polyneuropathy, sensory neurons, nuclear structure and spliceosome alterations: a role for CWC22. Dis Model Mech. 2017;10:215-224 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:400; fig 3	Park S, Brenner D, Shin G, Morgan C, Copits B, Chung H, et al. Soft, stretchable, fully implantable miniaturized optoelectronic systems for wireless optogenetics. Nat Biotechnol. 2015;33:1280-1286 pubmed publisher
western blot; mouse; 1:200; fig 2	Blanchard J, Eade K, Szucs A, Lo Sardo V, Tsunemoto R, Williams D, et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci. 2015;18:25-35 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000	Kojundzic S, Puljak L, Hogan Q, Sapunar D. Depression of Ca(2+)/calmodulin-dependent protein kinase II in dorsal root ganglion neurons after spinal nerve ligation. J Comp Neurol. 2010;518:64-74 pubmed publisher
immunohistochemistry; rat; 1:500 immunohistochemistry; mouse; 1:500	Amadesi S, Grant A, Cottrell G, Vaksman N, Poole D, Rozengurt E, et al. Protein kinase D isoforms are expressed in rat and mouse primary sensory neurons and are activated by agonists of protease-activated receptor 2. J Comp Neurol. 2009;516:141-56 pubmed publisher
	Watson Scales S, Kalmar B, Lana Elola E, Gibbins D, La Russa F, Wiseman F, et al. Analysis of motor dysfunction in Down Syndrome reveals motor neuron degeneration. PLoS Genet. 2018;14:e1007383 pubmed publisher
	Repić T, Madirazza K, Bektur E, Sapunar D. Characterization of dorsal root ganglion neurons cultured on silicon micro-pillar substrates. Sci Rep. 2016;6:39560 pubmed publisher
	Rodriguez Franco E, Cantres Rosario Y, Plaud Valentin M, Romeu R, Rodriguez Y, Skolasky R, et al. Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosis. PLoS ONE. 2012;7:e36571 pubmed publisher
	Patel A, Krimm R. Neurotrophin-4 regulates the survival of gustatory neurons earlier in development using a different mechanism than brain-derived neurotrophic factor. Dev Biol. 2012;365:50-60 pubmed publisher
	Ganay T, Boizot A, Burrer R, Chauvin J, Bomont P. Sensory-motor deficits and neurofilament disorganization in gigaxonin-null mice. Mol Neurodegener. 2011;6:25 pubmed publisher
	Knerlich Lukoschus F, von der Ropp Brenner B, Lucius R, Mehdorn H, Held Feindt J. Chemokine expression in the white matter spinal cord precursor niche after force-defined spinal cord contusion injuries in adult rats. Glia. 2010;58:916-31 pubmed publisher
	Patel A, Krimm R. BDNF is required for the survival of differentiated geniculate ganglion neurons. Dev Biol. 2010;340:419-29 pubmed publisher
	Mojumder D, Wensel T, Frishman L. Subcellular compartmentalization of two calcium binding proteins, calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina. Mol Vis. 2008;14:1600-13 pubmed
	Mojumder D, Sherry D, Frishman L. Contribution of voltage-gated sodium channels to the b-wave of the mammalian flash electroretinogram. J Physiol. 2008;586:2551-80 pubmed publisher
	Guipponi M, Toh M, Tan J, Park D, Hanson K, Ballana E, et al. An integrated genetic and functional analysis of the role of type II transmembrane serine proteases (TMPRSSs) in hearing loss. Hum Mutat. 2008;29:130-41 pubmed
	Tan J, Shepherd R. Aminoglycoside-induced degeneration of adult spiral ganglion neurons involves differential modulation of tyrosine kinase B and p75 neurotrophin receptor signaling. Am J Pathol. 2006;169:528-43 pubmed

product information

Catalog Number :

MAB5266

Subcategory :

Neuroscience

Product Name :

Anti-Neurofilament 200 kDa Antibody, clone N52

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12036

Host Name :

Mouse

Antigen :

Neurofilament 200 kDa

Clone :

N52

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Neurofilament 200 kDa Antibody, clone N52

Cross Reactivity :

Human;Mouse;Monkey;Pig;Rat

Background :

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Immunogen :

Carboxy terminal tail segment of enzymatically dephosphorylated porcine H-chain.

Specificity :

MAB5266 reacts with the phophorylated and dephosphorylated H-chain of neurofilament 200kDa (NF-H) in normal tissues/extracts (Shaw, 1986). MAB5266 can be used to detect cells of neuronal origin by immunohistochemistry and Western blot. It has been reported that reactivity of MAB5266 with NF-H is blocked in cdk-5 over-expressing cells (Guidato, 1996).

Package Size :

50 µg

Uses :

Western Blotting;Immunohistochemistry

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.