This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Neurofilament 200 kDa Antibody, clone RT97
catalog :
MAB5262
quantity :
50 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RT97

The same clone is also sold as:
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 45
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3d
López Erauskin J, Tadokoro T, Baughn M, Myers B, McAlonis Downes M, Chillon Marinas C, et al. ALS/FTD-Linked Mutation in FUS Suppresses Intra-axonal Protein Synthesis and Drives Disease Without Nuclear Loss-of-Function of FUS. Neuron. 2018;100:816-830.e7 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 3
Kawabata S, Takano M, Numasawa Kuroiwa Y, Itakura G, Kobayashi Y, Nishiyama Y, et al. Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury. Stem Cell Reports. 2016;6:1-8 pubmed publisher
  • immunocytochemistry; mouse; 1:500; tbl 1
Hirata H, Umemori J, Yoshioka H, Koide T, Watanabe K, Shimoda Y. Cell adhesion molecule contactin-associated protein 3 is expressed in the mouse basal ganglia during early postnatal stages. J Neurosci Res. 2016;94:74-89 pubmed publisher
  • immunohistochemistry; rat
Chen W, Chen C, Chen N, Sung C, Wen Z. Neuroprotective Effects of Direct Intrathecal Administration of Granulocyte Colony-Stimulating Factor in Rats with Spinal Cord Injury. CNS Neurosci Ther. 2015;21:698-707 pubmed publisher
  • immunohistochemistry; mouse; 1:800; fig 2
McCoy E, Street S, Taylor Blake B, Yi J, Edwards M, Wightman M, et al. Deletion of ENTPD3 does not impair nucleotide hydrolysis in primary somatosensory neurons or spinal cord. F1000Res. 2014;3:163 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:200; loading ...; fig 5
  • immunocytochemistry; human; 1:500; loading ...; fig 1b
Lee H, Kim K, Lim H, Choi M, Kim H, Ahn H, et al. Priming Wharton's jelly-derived mesenchymal stromal/stem cells with ROCK inhibitor improves recovery in an intracerebral hemorrhage model. J Cell Biochem. 2015;116:310-9 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:200
  • western blot; rat
Zang Y, Chen S, Liao G, Zhu H, Wei X, Cui Y, et al. Calpain-2 contributes to neuropathic pain following motor nerve injury via up-regulating interleukin-6 in DRG neurons. Brain Behav Immun. 2015;44:37-47 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:2000
Oklinski M, Lim J, Choi H, Oklinska P, Skowronski M, Kwon T. Immunolocalization of Water Channel Proteins AQP1 and AQP4 in Rat Spinal Cord. J Histochem Cytochem. 2014;62:598-611 pubmed publisher
Pradier B, Shin H, Kim D, St Laurent R, Lipscombe D, Kauer J. Long-Term Depression Induced by Optogenetically Driven Nociceptive Inputs to Trigeminal Nucleus Caudalis or Headache Triggers. J Neurosci. 2018;38:7529-7540 pubmed publisher
Ouyang H, Nie B, Wang P, Li Q, Huang W, Xin W, et al. Ulinastatin attenuates neuropathic pain induced by L5-VRT via the calcineurin/IL-10 pathway. Mol Pain. 2016;12: pubmed publisher
Patzke C, Acuna C, Giam L, Wernig M, Südhof T. Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation. J Exp Med. 2016;213:499-515 pubmed publisher
Musick K, Rigosa J, Narasimhan S, Wurth S, Capogrosso M, Chew D, et al. Chronic multichannel neural recordings from soft regenerative microchannel electrodes during gait. Sci Rep. 2015;5:14363 pubmed publisher
Nicholls S, Copland D, Vítová A, Kuffova L, Forrester J, Dick A. Local targeting of the CD200-CD200R axis does not promote corneal graft survival. Exp Eye Res. 2015;130:1-8 pubmed publisher
Li M, Liu J, Wang S, Xu H, Cui L, Lv S, et al. Multipotent neural crest stem cell-like cells from rat vibrissa dermal papilla induce neuronal differentiation of PC12 cells. Biomed Res Int. 2014;2014:186239 pubmed publisher
Tomassoni D, Amenta F, Di Cesare Mannelli L, Ghelardini C, Nwankwo I, Pacini A, et al. Neuroprotective activity of thioctic acid in central nervous system lesions consequent to peripheral nerve injury. Biomed Res Int. 2013;2013:985093 pubmed publisher
Kanno H, Pressman Y, Moody A, Berg R, Muir E, Rogers J, et al. Combination of engineered Schwann cell grafts to secrete neurotrophin and chondroitinase promotes axonal regeneration and locomotion after spinal cord injury. J Neurosci. 2014;34:1838-55 pubmed publisher
Flowerdew S, Wick D, Himmelein S, Horn A, Sinicina I, Strupp M, et al. Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection. PLoS ONE. 2013;8:e83603 pubmed publisher
de Carlos F, Cobo J, Macias E, Feito J, Cobo T, Calavia M, et al. The sensory innervation of the human pharynx: searching for mechanoreceptors. Anat Rec (Hoboken). 2013;296:1735-46 pubmed publisher
Do J, Bonni A, Tuszynski M. SnoN facilitates axonal regeneration after spinal cord injury. PLoS ONE. 2013;8:e71906 pubmed publisher
Zoupi L, Markoullis K, Kleopa K, Karagogeos D. Alterations of juxtaparanodal domains in two rodent models of CNS demyelination. Glia. 2013;61:1236-49 pubmed publisher
Samaranch L, Salegio E, San Sebastián W, Kells A, Bringas J, Forsayeth J, et al. Strong cortical and spinal cord transduction after AAV7 and AAV9 delivery into the cerebrospinal fluid of nonhuman primates. Hum Gene Ther. 2013;24:526-32 pubmed publisher
Tomassoni D, Amenta F, Amantini C, Farfariello V, Di Cesare Mannelli L, Nwankwo I, et al. Brain activity of thioctic Acid enantiomers: in vitro and in vivo studies in an animal model of cerebrovascular injury. Int J Mol Sci. 2013;14:4580-95 pubmed publisher
Allodi I, Casals Díaz L, Santos Nogueira E, Gonzalez Perez F, Navarro X, Udina E. FGF-2 low molecular weight selectively promotes neuritogenesis of motor neurons in vitro. Mol Neurobiol. 2013;47:770-81 pubmed publisher
Wei X, Na X, Liao G, Chen Q, Cui Y, Chen F, et al. The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection. Exp Neurol. 2013;241:159-68 pubmed publisher
Park S, Ki C, Park Y, Lee K, Kang S, Kweon H, et al. Functional recovery guided by an electrospun silk fibroin conduit after sciatic nerve injury in rats. J Tissue Eng Regen Med. 2015;9:66-76 pubmed publisher
Beaumont T, Yao B, Shah A, Kapatos G, Loeb J. Layer-specific CREB target gene induction in human neocortical epilepsy. J Neurosci. 2012;32:14389-401 pubmed publisher
Konomi T, Fujiyoshi K, Hikishima K, Komaki Y, Tsuji O, Okano H, et al. Conditions for quantitative evaluation of injured spinal cord by in vivo diffusion tensor imaging and tractography: preclinical longitudinal study in common marmosets. Neuroimage. 2012;63:1841-53 pubmed publisher
Gelpi E, Lladó A, Clarimon J, Rey M, Rivera R, Ezquerra M, et al. Phenotypic variability within the inclusion body spectrum of basophilic inclusion body disease and neuronal intermediate filament inclusion disease in frontotemporal lobar degenerations with FUS-positive inclusions. J Neuropathol Exp Neurol. 2012;71:795-805 pubmed publisher
Vilas D, Marti M, Botta Orfila T, Colom Cadena M, Gelpi E. Pick's pathology in Parkinson's disease with dementia. Neuropathol Appl Neurobiol. 2012;38:737-43 pubmed publisher
Wang Y, Ledgerwood C, Grills C, Fitzgerald D, Hamilton P. A robust co-localisation measurement utilising z-stack image intensity similarities for biological studies. PLoS ONE. 2012;7:e30632 pubmed publisher
Nitzan Luques A, Devor M, Tal M. Genotype-selective phenotypic switch in primary afferent neurons contributes to neuropathic pain. Pain. 2011;152:2413-26 pubmed publisher
Elbrønd Bek H, Wellejus A, Kelly N, Weidner M, Jørgensen S. 2-(Cyclohexylamino)-1-(4-cyclopentylpiperazin-1-yl)-2-methylpropan-1-one, a novel compound with neuroprotective and neurotrophic effects in vitro. Neurochem Int. 2011;59:821-9 pubmed publisher
Gaig C, Valldeoriola F, Gelpi E, Ezquerra M, Llufriu S, Buongiorno M, et al. Rapidly progressive diffuse Lewy body disease. Mov Disord. 2011;26:1316-23 pubmed publisher
Allodi I, Guzmán Lenis M, Hernandez J, Navarro X, Udina E. In vitro comparison of motor and sensory neuron outgrowth in a 3D collagen matrix. J Neurosci Methods. 2011;198:53-61 pubmed publisher
Chi G, Kim M, Kim D, Jiang M, Son Y. Schwann cells differentiated from spheroid-forming cells of rat subcutaneous fat tissue myelinate axons in the spinal cord injury. Exp Neurol. 2010;222:304-17 pubmed publisher
Jamieson S, Subramaniam J, Liu J, Jong N, Ip V, Connor B, et al. Oxaliplatin-induced loss of phosphorylated heavy neurofilament subunit neuronal immunoreactivity in rat DRG tissue. Mol Pain. 2009;5:66 pubmed publisher
Tayebati S, Di Tullio M, Tomassoni D, Amenta F. Neuroprotective effect of treatment with galantamine and choline alphoscerate on brain microanatomy in spontaneously hypertensive rats. J Neurol Sci. 2009;283:187-94 pubmed publisher
De Schepper H, de Winter B, van Nassauw L, Timmermans J, Herman A, Pelckmans P, et al. TRPV1 receptors on unmyelinated C-fibres mediate colitis-induced sensitization of pelvic afferent nerve fibres in rats. J Physiol. 2008;586:5247-58 pubmed publisher
Nishio T, Kawaguchi S, Fujiwara H. Emergence of highly neurofilament-immunoreactive zipper-like axon segments at the transection site in scalpel-cordotomized adult rats. Neuroscience. 2008;155:90-103 pubmed publisher
Geeta R, Ramnath R, Rao H, Chandra V. One year survival and significant reversal of motor deficits in parkinsonian rats transplanted with hESC derived dopaminergic neurons. Biochem Biophys Res Commun. 2008;373:258-64 pubmed publisher
Löw K, Culbertson M, Bradke F, Tessier Lavigne M, Tuszynski M. Netrin-1 is a novel myelin-associated inhibitor to axon growth. J Neurosci. 2008;28:1099-108 pubmed publisher
Raedt R, Pinxteren J, Van Dycke A, Waeytens A, Craeye D, Timmermans F, et al. Differentiation assays of bone marrow-derived Multipotent Adult Progenitor Cell (MAPC)-like cells towards neural cells cannot depend on morphology and a limited set of neural markers. Exp Neurol. 2007;203:542-54 pubmed
Nakao S, Uemura M, Aoki E, Suzuki S, Takeichi M, Hirano S. Distribution of OL-protocadherin in axon fibers in the developing chick nervous system. Brain Res Mol Brain Res. 2005;134:294-308 pubmed
Bampton E, Taylor J. Effects of Schwann cell secreted factors on PC12 cell neuritogenesis and survival. J Neurobiol. 2005;63:29-48 pubmed
Itoh K, Cheng L, Kamei Y, Fushiki S, Kamiguchi H, Gutwein P, et al. Brain development in mice lacking L1-L1 homophilic adhesion. J Cell Biol. 2004;165:145-54 pubmed
product information
Catalog Number :
MAB5262
Subcategory :
Neuroscience
Product Name :
Anti-Neurofilament 200 kDa Antibody, clone RT97
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P12036
Host Name :
Mouse
Antigen :
Neurofilament 200 kDa
Clone :
RT97
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Neurofilament 200 kDa Antibody, clone RT97
Cross Reactivity :
Human;Mouse;Rat
Background :
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section.
Immunogen :
Rat brain protein preparation
Specificity :
The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type).
Package Size :
50 μg
Uses :
Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Stable for 1 year at 2-8ºC from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
  • • Cancer
  • • Nuclear Signaling
  • • Apoptosis
  • • Cardiovascular Disease
  • • Developmental Biology
  • • Metabolic Disease
  • • Neurodegenerative Disease
  • • Cell Biology
View all the new antibodies at www.emdmillipore.com.