This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Synaptophysin
catalog :
MAB5258
conjugate :
nonconjugated
reactivity :
human, mouse, rat, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section, western blot knockout validation
citations: 54
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1
Raja M, Preobraschenski J, Del Olmo Cabrera S, Martínez Turrillas R, Jahn R, Pérez Otaño I, et al. Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts. elife. 2019;8: pubmed publisher
  • immunohistochemistry - free floating section; mouse; loading ...; fig 5b
Ou Yang M, Kurz J, Nomura T, Popovic J, Rajapaksha T, Dong H, et al. Axonal organization defects in the hippocampus of adult conditional BACE1 knockout mice. Sci Transl Med. 2018;10: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 9e
Han L, Wang L, Tang S, Yuan L, Wu S, Du X, et al. ITGB4 deficiency in bronchial epithelial cells directs airway inflammation and bipolar disorder-related behavior. J Neuroinflammation. 2018;15:246 pubmed publisher
  • immunohistochemistry; rat; 1:10,000; loading ...; fig s4a
Pocratsky A, Burke D, Morehouse J, Beare J, Riegler A, Tsoulfas P, et al. Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation. Nat Commun. 2017;8:1963 pubmed publisher
  • western blot; mouse; 1:10,000; loading ...; fig 4c
Tanabe Y, Naito Y, Vasuta C, Lee A, Soumounou Y, Linhoff M, et al. IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2?. Nat Commun. 2017;8:408 pubmed publisher
  • immunocytochemistry; mouse; 1:3000; fig s3d
Huang H, Lin X, Liang Z, Zhao T, Du S, Loy M, et al. Cdk5-dependent phosphorylation of liprin?1 mediates neuronal activity-dependent synapse development. Proc Natl Acad Sci U S A. 2017;114:E6992-E7001 pubmed publisher
  • immunohistochemistry; rat; 1:2000; loading ...; fig 8a
Lee H, White J, Chung J, Tansey K. Peripheral and central anatomical organization of cutaneous afferent subtypes in a rat nociceptive intersegmental spinal reflex. J Comp Neurol. 2017;525:2216-2234 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig s7
Klementieva O, Willén K, Martinsson I, Israelsson B, Engdahl A, Cladera J, et al. Pre-plaque conformational changes in Alzheimer's disease-linked Aβ and APP. Nat Commun. 2017;8:14726 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 4a
Le H, Ahn B, Lee H, Shin A, Chae S, Lee S, et al. Disruption of Ninjurin1 Leads to Repetitive and Anxiety-Like Behaviors in Mice. Mol Neurobiol. 2017;54:7353-7368 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:500; fig 2
Vidigal de Castro M, Barbizan R, Seabra Ferreira R, Barraviera B, Leite Rodrigues de Oliveira A. Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration. Neural Plast. 2016;2016:2932784 pubmed publisher
  • immunohistochemistry - free floating section; mouse; loading ...; fig s5a
  • western blot; mouse; loading ...; fig 5h
Yan S, Du F, Wu L, Zhang Z, Zhong C, Yu Q, et al. F1F0 ATP Synthase-Cyclophilin D Interaction Contributes to Diabetes-Induced Synaptic Dysfunction and Cognitive Decline. Diabetes. 2016;65:3482-3494 pubmed
  • immunohistochemistry - frozen section; rat; loading ...; fig 4a
Wang W, Trieu B, Palmer L, Jia Y, Pham D, Jung K, et al. A Primary Cortical Input to Hippocampus Expresses a Pathway-Specific and Endocannabinoid-Dependent Form of Long-Term Potentiation. Eneuro. 2016;3: pubmed publisher
  • immunocytochemistry; rat; loading ...; fig 3
Li Y, Chang L, Song Y, Gao X, Roselli F, Liu J, et al. Astrocytic GluN2A and GluN2B Oppose the Synaptotoxic Effects of Amyloid-?1-40 in Hippocampal Cells. J Alzheimers Dis. 2016;54:135-48 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 3d
Mazzulli J, Zunke F, Tsunemi T, Toker N, Jeon S, Burbulla L, et al. Activation of β-Glucocerebrosidase Reduces Pathological α-Synuclein and Restores Lysosomal Function in Parkinson's Patient Midbrain Neurons. J Neurosci. 2016;36:7693-706 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:100; fig 11
Vernay A, Therreau L, Blot B, Risson V, Dirrig Grosch S, Waegaert R, et al. A transgenic mouse expressing CHMP2Bintron5 mutant in neurons develops histological and behavioural features of amyotrophic lateral sclerosis and frontotemporal dementia. Hum Mol Genet. 2016;25:3341-3360 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
  • immunocytochemistry; mouse; fig 3
  • western blot; mouse; fig 2
Hayashi Y, Nishimune H, Hozumi K, Saga Y, Harada A, Yuzaki M, et al. A novel non-canonical Notch signaling regulates expression of synaptic vesicle proteins in excitatory neurons. Sci Rep. 2016;6:23969 pubmed publisher
  • immunohistochemistry; mouse; 1:300; loading ...; fig 6a
Sadleir K, Kandalepas P, Buggia Prevot V, Nicholson D, Thinakaran G, Vassar R. Presynaptic dystrophic neurites surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aβ generation in Alzheimer's disease. Acta Neuropathol. 2016;132:235-56 pubmed publisher
  • immunocytochemistry; human; 1:100; tbl 1
de Souza C, Nivison Smith L, Christie D, Polkinghorne P, McGhee C, Kalloniatis M, et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp Eye Res. 2016;150:135-48 pubmed publisher
  • immunocytochemistry; rat; fig 2
Tagliatti E, Fadda M, Falace A, Benfenati F, Fassio A. Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse. elife. 2016;5: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 3
Caccamo A, Branca C, Talboom J, Shaw D, Turner D, Ma L, et al. Reducing Ribosomal Protein S6 Kinase 1 Expression Improves Spatial Memory and Synaptic Plasticity in a Mouse Model of Alzheimer's Disease. J Neurosci. 2015;35:14042-56 pubmed publisher
  • immunocytochemistry; rat; 1:200; fig 2
Takahashi N, Sawada W, Noguchi J, Watanabe S, Ucar H, Hayashi Takagi A, et al. Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and β cells. Nat Commun. 2015;6:8531 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 5c
  • western blot; mouse; 1:1000; loading ...; fig 5a
Bhatt D, Puig K, Gorr M, Wold L, Combs C. A pilot study to assess effects of long-term inhalation of airborne particulate matter on early Alzheimer-like changes in the mouse brain. PLoS ONE. 2015;10:e0127102 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig s3a
Frank C, Liu F, Wijayatunge R, Song L, Biegler M, Yang M, et al. Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum. Nat Neurosci. 2015;18:647-56 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 8
Yamanaka T, Tosaki A, Kurosawa M, Akimoto K, Hirose T, Ohno S, et al. Loss of aPKC? in differentiated neurons disrupts the polarity complex but does not induce obvious neuronal loss or disorientation in mouse brains. PLoS ONE. 2013;8:e84036 pubmed publisher
Van Skike C, Lin A, Roberts Burbank R, Halloran J, Hernandez S, Cuvillier J, et al. mTOR drives cerebrovascular, synaptic, and cognitive dysfunction in normative aging. Aging Cell. 2020;19:e13057 pubmed publisher
Wang X, Li R, Zacharek A, Landschoot Ward J, Wang F, Wu K, et al. Administration of Downstream ApoE Attenuates the Adverse Effect of Brain ABCA1 Deficiency on Stroke. Int J Mol Sci. 2018;19: pubmed publisher
Maimon R, Ionescu A, Bonnie A, Sweetat S, Wald Altman S, Inbar S, et al. miR126-5p Downregulation Facilitates Axon Degeneration and NMJ Disruption via a Non-Cell-Autonomous Mechanism in ALS. J Neurosci. 2018;38:5478-5494 pubmed publisher
Rotem N, Magen I, Ionescu A, Gershoni Emek N, Altman T, Costa C, et al. ALS Along the Axons - Expression of Coding and Noncoding RNA Differs in Axons of ALS models. Sci Rep. 2017;7:44500 pubmed publisher
Tao T, Sondalle S, Shi H, Zhu S, Perez Atayde A, Peng J, et al. The pre-rRNA processing factor DEF is rate limiting for the pathogenesis of MYCN-driven neuroblastoma. Oncogene. 2017;36:3852-3867 pubmed publisher
Zhang Y, Zhang Z, Chopp M, Meng Y, Zhang L, Mahmood A, et al. Treatment of traumatic brain injury in rats with N-acetyl-seryl-aspartyl-lysyl-proline. J Neurosurg. 2017;126:782-795 pubmed publisher
Bryant A, Moore D, Burdo T, Lakritz J, Gouaux B, Soontornniyomkij V, et al. Plasma soluble CD163 is associated with postmortem brain pathology in human immunodeficiency virus infection. AIDS. 2017;31:973-979 pubmed publisher
Belmer A, Klenowski P, Patkar O, Bartlett S. Mapping the connectivity of serotonin transporter immunoreactive axons to excitatory and inhibitory neurochemical synapses in the mouse limbic brain. Brain Struct Funct. 2017;222:1297-1314 pubmed publisher
Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed publisher
Li H, Wu C, Aramayo R, Sachs M, Harlow M. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep. 2015;5:14918 pubmed publisher
Suslak T, Watson S, Thompson K, Shenton F, Bewick G, Armstrong J, et al. Piezo Is Essential for Amiloride-Sensitive Stretch-Activated Mechanotransduction in Larval Drosophila Dorsal Bipolar Dendritic Sensory Neurons. PLoS ONE. 2015;10:e0130969 pubmed publisher
Zhou H, Liu G. Regulation of density of functional presynaptic terminals by local energy supply. Mol Brain. 2015;8:42 pubmed publisher
Sun Y, Florer J, Mayhew C, Jia Z, Zhao Z, Xu K, et al. Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology. PLoS ONE. 2015;10:e0118771 pubmed publisher
Höllerhage M, Deck R, de Andrade A, Respondek G, Xu H, Rösler T, et al. Piericidin A aggravates Tau pathology in P301S transgenic mice. PLoS ONE. 2014;9:e113557 pubmed publisher
Akkerman S, Prickaerts J, Bruder A, Wolfs K, De Vry J, Vanmierlo T, et al. PDE5 inhibition improves object memory in standard housed rats but not in rats housed in an enriched environment: implications for memory models?. PLoS ONE. 2014;9:e111692 pubmed publisher
Fuchs C, Trazzi S, Torricella R, Viggiano R, De Franceschi M, Amendola E, et al. Loss of CDKL5 impairs survival and dendritic growth of newborn neurons by altering AKT/GSK-3? signaling. Neurobiol Dis. 2014;70:53-68 pubmed publisher
Kuypers E, Jellema R, Ophelders D, Dudink J, Nikiforou M, Wolfs T, et al. Effects of intra-amniotic lipopolysaccharide and maternal betamethasone on brain inflammation in fetal sheep. PLoS ONE. 2013;8:e81644 pubmed publisher
Xin H, Li Y, Cui Y, Yang J, Zhang Z, Chopp M. Systemic administration of exosomes released from mesenchymal stromal cells promote functional recovery and neurovascular plasticity after stroke in rats. J Cereb Blood Flow Metab. 2013;33:1711-5 pubmed publisher
Hajjar T, Goh Y, Rajion M, Vidyadaran S, Li T, Ebrahimi M. Alterations in neuronal morphology and synaptophysin expression in the rat brain as a result of changes in dietary n-6: n-3 fatty acid ratios. Lipids Health Dis. 2013;12:113 pubmed publisher
Zlatic S, Grossniklaus E, Ryder P, Salazar G, Mattheyses A, Peden A, et al. Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis. Mol Biol Cell. 2013;24:2378-88 pubmed publisher
Yoneda T, Kameyama K, Esumi K, Daimyo Y, Watanabe M, Hata Y. Developmental and visual input-dependent regulation of the CB1 cannabinoid receptor in the mouse visual cortex. PLoS ONE. 2013;8:e53082 pubmed publisher
López del Hoyo N, Fazioli L, López Begines S, Fernández Sánchez L, Cuenca N, Llorens J, et al. Overexpression of guanylate cyclase activating protein 2 in rod photoreceptors in vivo leads to morphological changes at the synaptic ribbon. PLoS ONE. 2012;7:e42994 pubmed publisher
Shin J, Padmanabhan A, de Groh E, Lee J, Haidar S, Dahlberg S, et al. Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development. Dis Model Mech. 2012;5:881-94 pubmed publisher
Chao H, Lai Y, Lu Y, Lin C, Mai W, Huang Y. NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3. Nucleic Acids Res. 2012;40:8484-98 pubmed
Ravache M, Weber C, Merienne K, Trottier Y. Transcriptional activation of REST by Sp1 in Huntington's disease models. PLoS ONE. 2010;5:e14311 pubmed publisher
Peng S, Lai Y, Huang H, Huang H, Huang Y. A novel role of CPEB3 in regulating EGFR gene transcription via association with Stat5b in neurons. Nucleic Acids Res. 2010;38:7446-57 pubmed publisher
Xin H, Li Y, Shen L, Liu X, Wang X, Zhang J, et al. Increasing tPA activity in astrocytes induced by multipotent mesenchymal stromal cells facilitate neurite outgrowth after stroke in the mouse. PLoS ONE. 2010;5:e9027 pubmed publisher
Chen W, Bergsman J, Wang X, Gilkey G, Pierpoint C, Daniel E, et al. Presynaptic external calcium signaling involves the calcium-sensing receptor in neocortical nerve terminals. PLoS ONE. 2010;5:e8563 pubmed publisher
Tong Y, Xu Y, Scearce Levie K, Ptacek L, Fu Y. COL25A1 triggers and promotes Alzheimer's disease-like pathology in vivo. Neurogenetics. 2010;11:41-52 pubmed publisher
Martínez Navarrete G, Martín Nieto J, Esteve Rudd J, Angulo A, Cuenca N. Alpha synuclein gene expression profile in the retina of vertebrates. Mol Vis. 2007;13:949-61 pubmed
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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