This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Synaptophysin Antibody, clone SY38
catalog :
MAB5258-20UG
quantity :
20 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SY38

The same clone is also sold as:
reactivity :
Pacific electric ray, human, mouse, rat, bovine, cat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 63
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 9e
Han L, Wang L, Tang S, Yuan L, Wu S, Du X, et al. ITGB4 deficiency in bronchial epithelial cells directs airway inflammation and bipolar disorder-related behavior. J Neuroinflammation. 2018;15:246 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 6c
Fuchs C, Fustini N, Trazzi S, Gennaccaro L, Rimondini R, Ciani E. Treatment with the GSK3-beta inhibitor Tideglusib improves hippocampal development and memory performance in juvenile, but not adult, Cdkl5 knockout mice. Eur J Neurosci. 2018;47:1054-1066 pubmed publisher
  • immunohistochemistry; rat; 1:10,000; loading ...; fig s4a
Pocratsky A, Burke D, Morehouse J, Beare J, Riegler A, Tsoulfas P, et al. Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation. Nat Commun. 2017;8:1963 pubmed publisher
  • western blot; mouse; 1:10,000
  • western blot; rat
Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:200
  • western blot; mouse; 1:5000
Wang D, Kinoshita Y, Kinoshita C, Uo T, Sopher B, Cudaback E, et al. Loss of endophilin-B1 exacerbates Alzheimer's disease pathology. Brain. 2015;138:2005-19 pubmed publisher
  • immunohistochemistry; human; 1:1000
Böttner M, Fricke T, Muller M, Barrenschee M, Deuschl G, Schneider S, et al. Alpha-synuclein is associated with the synaptic vesicle apparatus in the human and rat enteric nervous system. Brain Res. 2015;1614:51-9 pubmed publisher
  • western blot; rat
Chen L, Wang Y, Chen J, Tseng G. NMDA receptor triggered molecular cascade underlies compression-induced rapid dendritic spine plasticity in cortical neurons. Exp Neurol. 2015;266:86-98 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 4a
Boska M, Dash P, Knibbe J, Epstein A, Akhter S, Fields N, et al. Associations between brain microstructures, metabolites, and cognitive deficits during chronic HIV-1 infection of humanized mice. Mol Neurodegener. 2014;9:58 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:2000
Höllerhage M, Deck R, de Andrade A, Respondek G, Xu H, Rösler T, et al. Piericidin A aggravates Tau pathology in P301S transgenic mice. PLoS ONE. 2014;9:e113557 pubmed publisher
  • immunocytochemistry; human
Pamies D, Bal Price A, Fabbri M, Gribaldo L, Scelfo B, Harris G, et al. Silencing of PNPLA6, the neuropathy target esterase (NTE) codifying gene, alters neurodifferentiation of human embryonal carcinoma stem cells (NT2). Neuroscience. 2014;281:54-67 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:150
König N, Trolle C, Kapuralin K, Adameyko I, Mitrecic D, Aldskogius H, et al. Murine neural crest stem cells and embryonic stem cell-derived neuron precursors survive and differentiate after transplantation in a model of dorsal root avulsion. J Tissue Eng Regen Med. 2017;11:129-137 pubmed publisher
  • western blot; Pacific electric ray
Li H, Harlow M. Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP. Physiol Rep. 2014;2:e00206 pubmed publisher
  • immunohistochemistry - frozen section; cat; 1:1000
Williams P, Kim S, Martin J. Postnatal maturation of the red nucleus motor map depends on rubrospinal connections with forelimb motor pools. J Neurosci. 2014;34:4432-41 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:2,000
Oda S, Funato H, Sato F, Adachi Akahane S, Ito M, Takase K, et al. A subset of thalamocortical projections to the retrosplenial cortex possesses two vesicular glutamate transporter isoforms, VGluT1 and VGluT2, in axon terminals and somata. J Comp Neurol. 2014;522:2089-106 pubmed publisher
  • western blot; rat; 1 ug/ml
Shenton F, Pyner S. Expression of transient receptor potential channels TRPC1 and TRPV4 in venoatrial endocardium of the rat heart. Neuroscience. 2014;267:195-204 pubmed publisher
  • western blot; mouse; 1:1,000,000
Eagleson K, Milner T, Xie Z, Levitt P. Synaptic and extrasynaptic location of the receptor tyrosine kinase met during postnatal development in the mouse neocortex and hippocampus. J Comp Neurol. 2013;521:3241-59 pubmed publisher
  • immunocytochemistry; human
Ruzicka J, Romanyuk N, Hejcl A, Vetrik M, Hruby M, Cocks G, et al. Treating spinal cord injury in rats with a combination of human fetal neural stem cells and hydrogels modified with serotonin. Acta Neurobiol Exp (Wars). 2013;73:102-15 pubmed
  • western blot; mouse
Chao H, Lai Y, Lu Y, Lin C, Mai W, Huang Y. NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3. Nucleic Acids Res. 2012;40:8484-98 pubmed
  • immunohistochemistry - frozen section; rat; 1:400
Issa A, Zhan W, Sieck G, Mantilla C. Neuregulin-1 at synapses on phrenic motoneurons. J Comp Neurol. 2010;518:4213-25 pubmed publisher
  • western blot; mouse; fig 5
Han M, Lin C, Meng S, Wang X. Proteomics analysis reveals overlapping functions of clustered protocadherins. Mol Cell Proteomics. 2010;9:71-83 pubmed publisher
Dalmo J, Spetz J, Montelius M, Langen B, Arvidsson Y, Johansson H, et al. Priming increases the anti-tumor effect and therapeutic window of 177Lu-octreotate in nude mice bearing human small intestine neuroendocrine tumor GOT1. EJNMMI Res. 2017;7:6 pubmed publisher
Rockenstein E, Overk C, Ubhi K, Mante M, Patrick C, Adame A, et al. A novel triple repeat mutant tau transgenic model that mimics aspects of pick's disease and fronto-temporal tauopathies. PLoS ONE. 2015;10:e0121570 pubmed publisher
Smith S, Figley S, Schreyer D, Paterson P. Protein-energy malnutrition developing after global brain ischemia induces an atypical acute-phase response and hinders expression of GAP-43. PLoS ONE. 2014;9:e107570 pubmed publisher
Dixon Salazar T, Fourgeaud L, Tyler C, Poole J, Park J, Boulanger L. MHC class I limits hippocampal synapse density by inhibiting neuronal insulin receptor signaling. J Neurosci. 2014;34:11844-56 pubmed publisher
Moreno Piovano G, Varayoud J, Luque E, Ramos J. Long-term ovariectomy increases BDNF gene methylation status in mouse hippocampus. J Steroid Biochem Mol Biol. 2014;144 Pt B:243-52 pubmed publisher
Games D, Valera E, Spencer B, Rockenstein E, Mante M, Adame A, et al. Reducing C-terminal-truncated alpha-synuclein by immunotherapy attenuates neurodegeneration and propagation in Parkinson's disease-like models. J Neurosci. 2014;34:9441-54 pubmed publisher
Crescenzi R, Debrosse C, Nanga R, Reddy S, Haris M, Hariharan H, et al. In vivo measurement of glutamate loss is associated with synapse loss in a mouse model of tauopathy. Neuroimage. 2014;101:185-92 pubmed publisher
Fuchs C, Trazzi S, Torricella R, Viggiano R, De Franceschi M, Amendola E, et al. Loss of CDKL5 impairs survival and dendritic growth of newborn neurons by altering AKT/GSK-3? signaling. Neurobiol Dis. 2014;70:53-68 pubmed publisher
Wang N, Gray M, Lu X, Cantle J, Holley S, Greiner E, et al. Neuronal targets for reducing mutant huntingtin expression to ameliorate disease in a mouse model of Huntington's disease. Nat Med. 2014;20:536-41 pubmed publisher
Rockenstein E, Nuber S, Overk C, Ubhi K, Mante M, Patrick C, et al. Accumulation of oligomer-prone ?-synuclein exacerbates synaptic and neuronal degeneration in vivo. Brain. 2014;137:1496-513 pubmed publisher
Sierksma A, Van den Hove D, Pfau F, Philippens M, Bruno O, Fedele E, et al. Improvement of spatial memory function in APPswe/PS1dE9 mice after chronic inhibition of phosphodiesterase type 4D. Neuropharmacology. 2014;77:120-30 pubmed publisher
Alexiou G, Moschovi M, Stefanaki K, Panagopoulos D, Tsotra M, Siozos G, et al. Supratentorial ependymomas in children: Analysis of nine cases. J Pediatr Neurosci. 2013;8:15-8 pubmed publisher
Zlatic S, Grossniklaus E, Ryder P, Salazar G, Mattheyses A, Peden A, et al. Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis. Mol Biol Cell. 2013;24:2378-88 pubmed publisher
Nakajima C, Kulik A, Frotscher M, Herz J, Schafer M, Bock H, et al. Low density lipoprotein receptor-related protein 1 (LRP1) modulates N-methyl-D-aspartate (NMDA) receptor-dependent intracellular signaling and NMDA-induced regulation of postsynaptic protein complexes. J Biol Chem. 2013;288:21909-23 pubmed publisher
Larimore J, Ryder P, Kim K, Ambrose L, Chapleau C, Calfa G, et al. MeCP2 regulates the synaptic expression of a Dysbindin-BLOC-1 network component in mouse brain and human induced pluripotent stem cell-derived neurons. PLoS ONE. 2013;8:e65069 pubmed publisher
Stagni F, Magistretti J, Guidi S, Ciani E, Mangano C, Calza L, et al. Pharmacotherapy with fluoxetine restores functional connectivity from the dentate gyrus to field CA3 in the Ts65Dn mouse model of down syndrome. PLoS ONE. 2013;8:e61689 pubmed publisher
Bachstetter A, Rowe R, Kaneko M, Goulding D, Lifshitz J, Van Eldik L. The p38? MAPK regulates microglial responsiveness to diffuse traumatic brain injury. J Neurosci. 2013;33:6143-53 pubmed publisher
Garringer H, Murrell J, Sammeta N, Gnezda A, Ghetti B, Vidal R. Increased tau phosphorylation and tau truncation, and decreased synaptophysin levels in mutant BRI2/tau transgenic mice. PLoS ONE. 2013;8:e56426 pubmed publisher
Nakata Y, Yasuda T, Fukaya M, Yamamori S, Itakura M, Nihira T, et al. Accumulation of ?-synuclein triggered by presynaptic dysfunction. J Neurosci. 2012;32:17186-96 pubmed publisher
Mitchell D, Blasier K, Jeffery E, Ross M, Pullikuth A, Suo D, et al. Trk activation of the ERK1/2 kinase pathway stimulates intermediate chain phosphorylation and recruits cytoplasmic dynein to signaling endosomes for retrograde axonal transport. J Neurosci. 2012;32:15495-510 pubmed publisher
Hitt B, Riordan S, Kukreja L, Eimer W, Rajapaksha T, Vassar R. ?-Site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1)-deficient mice exhibit a close homolog of L1 (CHL1) loss-of-function phenotype involving axon guidance defects. J Biol Chem. 2012;287:38408-25 pubmed publisher
Bachstetter A, Norris C, Sompol P, Wilcock D, Goulding D, Neltner J, et al. Early stage drug treatment that normalizes proinflammatory cytokine production attenuates synaptic dysfunction in a mouse model that exhibits age-dependent progression of Alzheimer's disease-related pathology. J Neurosci. 2012;32:10201-10 pubmed publisher
Wang C, Huang Y. Calpain 2 activated through N-methyl-D-aspartic acid receptor signaling cleaves CPEB3 and abrogates CPEB3-repressed translation in neurons. Mol Cell Biol. 2012;32:3321-32 pubmed publisher
Orozco D, Tahirovic S, Rentzsch K, Schwenk B, Haass C, Edbauer D. Loss of fused in sarcoma (FUS) promotes pathological Tau splicing. EMBO Rep. 2012;13:759-64 pubmed publisher
Nunes A, Amaral J, Lo A, Fonseca M, Viana R, Callaerts Vegh Z, et al. TUDCA, a bile acid, attenuates amyloid precursor protein processing and amyloid-? deposition in APP/PS1 mice. Mol Neurobiol. 2012;45:440-54 pubmed publisher
Silasi G, Klahr A, Hackett M, Auriat A, Nichol H, Colbourne F. Prolonged therapeutic hypothermia does not adversely impact neuroplasticity after global ischemia in rats. J Cereb Blood Flow Metab. 2012;32:1525-34 pubmed publisher
Mandler M, Rockenstein E, Ubhi K, Hansen L, Adame A, Michael S, et al. Detection of peri-synaptic amyloid-? pyroglutamate aggregates in early stages of Alzheimer's disease and in A?PP transgenic mice using a novel monoclonal antibody. J Alzheimers Dis. 2012;28:783-94 pubmed publisher
Tang J, Mardini F, Caltagarone B, Garrity S, Li R, Bianchi S, et al. Anesthesia in presymptomatic Alzheimer's disease: a study using the triple-transgenic mouse model. Alzheimers Dement. 2011;7:521-531.e1 pubmed publisher
Bangasser D, Zhang X, Garachh V, Hanhauser E, Valentino R. Sexual dimorphism in locus coeruleus dendritic morphology: a structural basis for sex differences in emotional arousal. Physiol Behav. 2011;103:342-51 pubmed publisher
Peng W, Bao Y, Sawicki J. Epithelial cell-targeted transgene expression enables isolation of cyan fluorescent protein (CFP)-expressing prostate stem/progenitor cells. Transgenic Res. 2011;20:1073-86 pubmed publisher
Hao W, Liu Y, Liu S, Walter S, Grimm M, Kiliaan A, et al. Myeloid differentiation factor 88-deficient bone marrow cells improve Alzheimer's disease-related symptoms and pathology. Brain. 2011;134:278-92 pubmed publisher
Gil Bea F, Solas M, Mateos L, Winblad B, Ramirez M, Cedazo Minguez A. Cholinergic hypofunction impairs memory acquisition possibly through hippocampal Arc and BDNF downregulation. Hippocampus. 2011;21:999-1009 pubmed publisher
Kubinova S, Horak D, Kozubenko N, Vanecek V, Proks V, Price J, et al. The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors. Biomaterials. 2010;31:5966-75 pubmed publisher
Murray C, Sanderson D, Barkus C, Deacon R, Rawlins J, Bannerman D, et al. Systemic inflammation induces acute working memory deficits in the primed brain: relevance for delirium. Neurobiol Aging. 2012;33:603-616.e3 pubmed publisher
Huttner A, Kieran M, Yao X, Cruz L, Ladner J, Quayle K, et al. Clinicopathologic study of glioblastoma in children with neurofibromatosis type 1. Pediatr Blood Cancer. 2010;54:890-6 pubmed publisher
Gottschall P, Ajmo J, Eakin A, Howell M, Mehta H, Bailey L. Panel of synaptic protein ELISAs for evaluating neurological phenotype. Exp Brain Res. 2010;201:885-93 pubmed publisher
Newell Litwa K, Chintala S, Jenkins S, Pare J, McGaha L, Smith Y, et al. Hermansky-Pudlak protein complexes, AP-3 and BLOC-1, differentially regulate presynaptic composition in the striatum and hippocampus. J Neurosci. 2010;30:820-31 pubmed publisher
Paganoni S, Bernstein J, Ferreira A. Ror1-Ror2 complexes modulate synapse formation in hippocampal neurons. Neuroscience. 2010;165:1261-74 pubmed publisher
Salazar G, Falcon Perez J, Harrison R, Faundez V. SLC30A3 (ZnT3) oligomerization by dityrosine bonds regulates its subcellular localization and metal transport capacity. PLoS ONE. 2009;4:e5896 pubmed publisher
Tateno M, Kato S, Sakurai T, Nukina N, Takahashi R, Araki T. Mutant SOD1 impairs axonal transport of choline acetyltransferase and acetylcholine release by sequestering KAP3. Hum Mol Genet. 2009;18:942-55 pubmed publisher
Watts J, Drisaldi B, Ng V, Yang J, Strome B, Horne P, et al. The CNS glycoprotein Shadoo has PrP(C)-like protective properties and displays reduced levels in prion infections. EMBO J. 2007;26:4038-50 pubmed
Martínez Navarrete G, Martín Nieto J, Esteve Rudd J, Angulo A, Cuenca N. Alpha synuclein gene expression profile in the retina of vertebrates. Mol Vis. 2007;13:949-61 pubmed
Sano Y, Nakaya T, Pedrini S, Takeda S, Iijima Ando K, Iijima K, et al. Physiological mouse brain Abeta levels are not related to the phosphorylation state of threonine-668 of Alzheimer's APP. PLoS ONE. 2006;1:e51 pubmed
product information
Catalog Number :
MAB5258-20UG
Subcategory :
Neuroscience
Product Name :
Anti-Synaptophysin Antibody, clone SY38
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P08247
Host Name :
Mouse
Antigen :
Synaptophysin
Clone :
SY38
Conjugate :
Purified
Isotype :
IgG1κ
Product Description :
Anti-Synaptophysin Antibody, clone SY38
Cross Reactivity :
Avian;Bovine;Fish;Human;Mouse;Rat
Background :
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.
ALT Names :
synaptophysin;Synaptophysin
Immunogen :
Vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990).
Specificity :
The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
Package Size :
20 µg
Uses :
Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Western Blotting
Storage :
Stable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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