Neurofilament 200 kDa Antibody, clone NE14 | EMD Millipore MAB5256 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Neurofilament 200 kDa Antibody, clone NE14

catalog :

MAB5256

quantity :

40 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NE14

The same clone is also sold as:

MilliporeSigma: N5389, SAB4200811
Novus Biologicals: NBP2-44728-0.1mg, NBP2-47834-0.1mg

reactivity :

human, mouse, rat

application :

immunohistochemistry

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:250	Lee C, Umemori H. Suppression of epileptogenesis-associated changes in response to seizures in FGF22-deficient mice. Front Cell Neurosci. 2013;7:43 pubmed publisher
	Lee H, Cho S, Moon I. The non-canonical effect of N-acetyl-D-glucosamine kinase on the formation of neuronal dendrites. Mol Cells. 2014;37:248-56 pubmed publisher
	Bangratz M, Sarrazin N, Devaux J, Zambroni D, Echaniz Laguna A, Rene F, et al. A mouse model of Schwartz-Jampel syndrome reveals myelinating Schwann cell dysfunction with persistent axonal depolarization in vitro and distal peripheral nerve hyperexcitability when perlecan is lacking. Am J Pathol. 2012;180:2040-55 pubmed publisher
	Baiou D, Santha P, Avelino A, Charrua A, Bacskai T, Matesz K, et al. Neurochemical characterization of insulin receptor-expressing primary sensory neurons in wild-type and vanilloid type 1 transient receptor potential receptor knockout mice. J Comp Neurol. 2007;503:334-47 pubmed

product information

Catalog Number :

MAB5256

Subcategory :

Neuroscience

Product Name :

Anti-Neurofilament 200 kDa Antibody, clone NE14

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12036

Host Name :

Mouse

Antigen :

Neurofilament 200 kDa

Clone :

NE14

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Neurofilament 200 kDa Antibody, clone NE14

Cross Reactivity :

Human;Pig;Rat

Background :

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Immunogen :

Purified neurofilament polypeptides (Debus et al., 1983).

Specificity :

The antibody reacts with neurofilament 200 kD.

Package Size :

40 µg

Uses :

Immunohistochemistry

Storage :

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months.DO NOT FREEZE