Neurofilament 160 kDa Antibody, clone NN18 | EMD Millipore MAB5254 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Neurofilament 160 kDa Antibody, clone NN18

catalog :

MAB5254

quantity :

40 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NN18

The same clone is also sold as:

MilliporeSigma: N5264

reactivity :

human, mouse, rat, chicken, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:500; fig 6g immunohistochemistry; mouse; 1:500; loading ...; fig 3a	Zhang J, Chen S, Cai J, Hou Z, Wang X, Kachelmeier A, et al. Culture media-based selection of endothelial cells, pericytes, and perivascular-resident macrophage-like melanocytes from the young mouse vestibular system. Hear Res. 2017;345:10-22 pubmed publisher
immunohistochemistry - frozen section; rat; 1:200; loading ...; fig 6	Cudré Cung H, Zavadakova P, Do Vale Pereira S, Remacle N, Henry H, Ivanisevic J, et al. Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria. Mol Genet Metab. 2016;119:57-67 pubmed publisher
immunohistochemistry - paraffin section; human; fig 2	Wharton K, Quigley C, Themeles M, Dunstan R, Doyle K, Cahir McFarland E, et al. JC Polyomavirus Abundance and Distribution in Progressive Multifocal Leukoencephalopathy (PML) Brain Tissue Implicates Myelin Sheath in Intracerebral Dissemination of Infection. PLoS ONE. 2016;11:e0155897 pubmed publisher
immunohistochemistry; pigs ; 1:5000	Duque S, Arnold W, Odermatt P, Li X, Porensky P, Schmelzer L, et al. A large animal model of spinal muscular atrophy and correction of phenotype. Ann Neurol. 2015;77:399-414 pubmed publisher
	Lin C, Yoshida M, Li L, Ikenaka A, Oshima S, Nakagawa K, et al. iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases. JCI Insight. 2019;4: pubmed publisher
	Dittrich F, Ramenda C, Grillitsch D, Frankl Vilches C, Ko M, Hertel M, et al. Regulatory mechanisms of testosterone-stimulated song in the sensorimotor nucleus HVC of female songbirds. BMC Neurosci. 2014;15:128 pubmed publisher
	Walko G, Wögenstein K, Winter L, Fischer I, Feltri M, Wiche G. Stabilization of the dystroglycan complex in Cajal bands of myelinating Schwann cells through plectin-mediated anchorage to vimentin filaments. Glia. 2013;61:1274-87 pubmed publisher
	Jafari P, Braissant O, Zavadakova P, Henry H, Bonafe L, Ballhausen D. Ammonium accumulation and cell death in a rat 3D brain cell model of glutaric aciduria type I. PLoS ONE. 2013;8:e53735 pubmed publisher
	Yuan A, Sasaki T, Kumar A, Peterhoff C, Rao M, LIEM R, et al. Peripherin is a subunit of peripheral nerve neurofilaments: implications for differential vulnerability of CNS and peripheral nervous system axons. J Neurosci. 2012;32:8501-8 pubmed publisher
	Lin L, Nitschke Dragon D, Jin J, Tian X, Chu Y, SIGMUND C, et al. Decreased expression of neuronal nitric oxide synthase in the nucleus tractus solitarii inhibits sympathetically mediated baroreflex responses in rat. J Physiol. 2012;590:3545-59 pubmed publisher
	Audet J, Gowing G, Paradis R, Soucy G, Julien J. Ablation of proliferating cells in the CNS exacerbates motor neuron disease caused by mutant superoxide dismutase. PLoS ONE. 2012;7:e34932 pubmed publisher
	Magalon K, Zimmer C, Cayre M, Khaldi J, Bourbon C, Robles I, et al. Olesoxime accelerates myelination and promotes repair in models of demyelination. Ann Neurol. 2012;71:213-26 pubmed publisher
	Swarup V, Phaneuf D, Bareil C, Robertson J, Rouleau G, Kriz J, et al. Pathological hallmarks of amyotrophic lateral sclerosis/frontotemporal lobar degeneration in transgenic mice produced with TDP-43 genomic fragments. Brain. 2011;134:2610-26 pubmed publisher
	Ganay T, Boizot A, Burrer R, Chauvin J, Bomont P. Sensory-motor deficits and neurofilament disorganization in gigaxonin-null mice. Mol Neurodegener. 2011;6:25 pubmed publisher
	Aizawa T, Hasegawa K, Ohkumo T, Haga S, Ikeda K, Yoshikawa K. Neural stem cell-like gene expression in a mouse ependymoma cell line transformed by human BK polyomavirus. Cancer Sci. 2011;102:122-9 pubmed publisher
	Grimal S, Puech S, Wagener R, Venteo S, Carroll P, Fichard Carroll A. Collagen XXVIII is a distinctive component of the peripheral nervous system nodes of ranvier and surrounds nonmyelinating glial cells. Glia. 2010;58:1977-87 pubmed publisher
	Biondi O, Branchu J, Sanchez G, Lancelin C, Deforges S, Lopes P, et al. In vivo NMDA receptor activation accelerates motor unit maturation, protects spinal motor neurons, and enhances SMN2 gene expression in severe spinal muscular atrophy mice. J Neurosci. 2010;30:11288-99 pubmed publisher
	Shan X, Chiang P, Price D, Wong P. Altered distributions of Gemini of coiled bodies and mitochondria in motor neurons of TDP-43 transgenic mice. Proc Natl Acad Sci U S A. 2010;107:16325-30 pubmed publisher
	Kaplamadzhiev D, Hisha H, Adachi Y, Ikehara S, Tonchev A, Boneva N, et al. Bone marrow-derived stromal cells can express neuronal markers by DHA/GPR40 signaling. Biosci Trends. 2010;4:119-29 pubmed
	Huxtable A, Zwicker J, Alvares T, Ruangkittisakul A, Fang X, Hahn L, et al. Glia contribute to the purinergic modulation of inspiratory rhythm-generating networks. J Neurosci. 2010;30:3947-58 pubmed publisher
	Gowing G, Lalancette Hébert M, Audet J, Dequen F, Julien J. Macrophage colony stimulating factor (M-CSF) exacerbates ALS disease in a mouse model through altered responses of microglia expressing mutant superoxide dismutase. Exp Neurol. 2009;220:267-75 pubmed publisher
	Chung C, Koprich J, Siddiqi H, Isacson O. Dynamic changes in presynaptic and axonal transport proteins combined with striatal neuroinflammation precede dopaminergic neuronal loss in a rat model of AAV alpha-synucleinopathy. J Neurosci. 2009;29:3365-73 pubmed publisher
	Gowing G, Philips T, Van Wijmeersch B, Audet J, Dewil M, Van Den Bosch L, et al. Ablation of proliferating microglia does not affect motor neuron degeneration in amyotrophic lateral sclerosis caused by mutant superoxide dismutase. J Neurosci. 2008;28:10234-44 pubmed publisher
	Sekizawa S, Chen C, Bechtold A, Tabor J, Bric J, Pinkerton K, et al. Extended secondhand tobacco smoke exposure induces plasticity in nucleus tractus solitarius second-order lung afferent neurons in young guinea pigs. Eur J Neurosci. 2008;28:771-81 pubmed publisher
	Biondi O, Grondard C, Lecolle S, Deforges S, Pariset C, Lopes P, et al. Exercise-induced activation of NMDA receptor promotes motor unit development and survival in a type 2 spinal muscular atrophy model mouse. J Neurosci. 2008;28:953-62 pubmed publisher
	Millecamps S, Gowing G, Corti O, Mallet J, Julien J. Conditional NF-L transgene expression in mice for in vivo analysis of turnover and transport rate of neurofilaments. J Neurosci. 2007;27:4947-56 pubmed
	Gros Louis F, Dupre N, Dion P, Fox M, Laurent S, Verreault S, et al. Mutations in SYNE1 lead to a newly discovered form of autosomal recessive cerebellar ataxia. Nat Genet. 2007;39:80-5 pubmed
	Fedorov V, Hucker W, Dobrzynski H, Rosenshtraukh L, Efimov I. Postganglionic nerve stimulation induces temporal inhibition of excitability in rabbit sinoatrial node. Am J Physiol Heart Circ Physiol. 2006;291:H612-23 pubmed

product information

Catalog Number :

MAB5254

Subcategory :

Neuroscience

Product Name :

Anti-Neurofilament 160 kDa Antibody, clone NN18

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P07197

Host Name :

Mouse

Antigen :

Neurofilament 160 kDa

Clone :

NN18

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Neurofilament 160 kDa Antibody, clone NN18

Cross Reactivity :

Chicken;Guinea Pig;Mouse;Pig;Rat;Human

Background :

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Immunogen :

Purified neurofilament polypeptides.

Specificity :

The antibody reacts with neurofilament 160 kD (Debus, E., 1982; Shaw, G., 1984).

Package Size :

40 µg

Uses :

Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. Do not freeze.