Neurocan Antibody, clone 650.24 | EMD Millipore MAB5212 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Neurocan Antibody, clone 650.24

catalog :

MAB5212

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

650.24

The same clone is also sold as:

Invitrogen: MA1-5843
MyBioSource: MBS375211
LifeSpan Biosciences: LS-C82321
ImmuQuest: IQ321, IQ322
MilliporeSigma: N0913
Santa Cruz Biotechnology: sc-33663
Abnova: MAB2432

reactivity :

rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat	Chen W, Chen C, Chen N, Sung C, Wen Z. Neuroprotective Effects of Direct Intrathecal Administration of Granulocyte Colony-Stimulating Factor in Rats with Spinal Cord Injury. CNS Neurosci Ther. 2015;21:698-707 pubmed publisher
	Blosa M, Sonntag M, Bruckner G, Jager C, Seeger G, Matthews R, et al. Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body--implications for physiological functions. Neuroscience. 2013;228:215-34 pubmed publisher
	Jeong S, Kwon M, Lee H, Joe E, Lee J, Kim S, et al. Hepatocyte growth factor reduces astrocytic scar formation and promotes axonal growth beyond glial scars after spinal cord injury. Exp Neurol. 2012;233:312-22 pubmed publisher
	Andrews E, Richards R, Yin F, Viapiano M, Jakeman L. Alterations in chondroitin sulfate proteoglycan expression occur both at and far from the site of spinal contusion injury. Exp Neurol. 2012;235:174-87 pubmed publisher
	Haist V, Ulrich R, Kalkuhl A, Deschl U, Baumgartner W. Distinct spatio-temporal extracellular matrix accumulation within demyelinated spinal cord lesions in Theiler's murine encephalomyelitis. Brain Pathol. 2012;22:188-204 pubmed publisher
	Del Bigio M, Enno T. Effect of hydrocephalus on rat brain extracellular compartment. Cerebrospinal Fluid Res. 2008;5:12 pubmed publisher
	Mizuno H, Warita H, Aoki M, Itoyama Y. Accumulation of chondroitin sulfate proteoglycans in the microenvironment of spinal motor neurons in amyotrophic lateral sclerosis transgenic rats. J Neurosci Res. 2008;86:2512-23 pubmed publisher
	Laabs T, Wang H, Katagiri Y, McCann T, Fawcett J, Geller H. Inhibiting glycosaminoglycan chain polymerization decreases the inhibitory activity of astrocyte-derived chondroitin sulfate proteoglycans. J Neurosci. 2007;27:14494-501 pubmed
	Iaci J, Vecchione A, Zimber M, Caggiano A. Chondroitin sulfate proteoglycans in spinal cord contusion injury and the effects of chondroitinase treatment. J Neurotrauma. 2007;24:1743-59 pubmed
	Cullen D, STABENFELDT S, Simon C, Tate C, LaPlaca M. In vitro neural injury model for optimization of tissue-engineered constructs. J Neurosci Res. 2007;85:3642-51 pubmed
	Vitellaro Zuccarello L, Bosisio P, Mazzetti S, Monti C, De Biasi S. Differential expression of several molecules of the extracellular matrix in functionally and developmentally distinct regions of rat spinal cord. Cell Tissue Res. 2007;327:433-47 pubmed
	Maiya R, Ponomarev I, Linse K, Harris R, Mayfield R. Defining the dopamine transporter proteome by convergent biochemical and in silico analyses. Genes Brain Behav. 2007;6:97-106 pubmed

product information

Catalog Number :

MAB5212

Subcategory :

Neuroscience

Product Name :

Anti-Neurocan Antibody, clone 650.24

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O14594

Host Name :

Mouse

Antigen :

Neurocan

Clone :

650.24

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Neurocan Antibody, clone 650.24

Cross Reactivity :

Rat

Background :

Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.

ALT Names :

245 kDa Early Postnatal Core Glycoprotein

Immunogen :

Embryonic rat brain proteoglycans

Specificity :

Neurocan. The specificity of clone 650.24 was checked by western blots of brain lysates and purified neurocan; the antibody reacted with the identical bands that clones 1D1 and 1F6 (other neurocan monoclonals) reacted; furthermore, the staining pattern of brain sections with these antibodies were all identical. Finally 650.24 also recognized recombinant neurocan expressed in transfected 293 cells by western blot.

Package Size :

100 µg

Uses :

Immunoprecipitation;Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 6 months.