B23 Antibody, clone 3C9 | EMD Millipore MAB4500 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

B23 Antibody, clone 3C9

catalog :

MAB4500

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C9

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3d	Evsyukov V, Domanskyi A, Bierhoff H, Gispert S, Mustafa R, Schlaudraff F, et al. Genetic mutations linked to Parkinson's disease differentially control nucleolar activity in pre-symptomatic mouse models. Dis Model Mech. 2017;10:633-643 pubmed publisher
immunocytochemistry; human western blot; human	Gupte M, Tuck A, Sharma V, Williams K. Major differences between tumor and normal human cell fates after exposure to chemotherapeutic monofunctional alkylator. PLoS ONE. 2013;8:e74071 pubmed publisher
western blot; human	Ratnakumar K, Duarte L, LeRoy G, Hasson D, Smeets D, Vardabasso C, et al. ATRX-mediated chromatin association of histone variant macroH2A1 regulates ?-globin expression. Genes Dev. 2012;26:433-8 pubmed publisher
	Jin Y, An G, Ni J, Li S, Jia H. ATM-dependent E2F1 accumulation in the nucleolus is an indicator of ribosomal stress in early response to DNA damage. Cell Cycle. 2014;13:1627-38 pubmed publisher
	Domanskyi A, Geissler C, Vinnikov I, Alter H, Schober A, Vogt M, et al. Pten ablation in adult dopaminergic neurons is neuroprotective in Parkinson's disease models. FASEB J. 2011;25:2898-910 pubmed publisher
	Rieker C, Engblom D, Kreiner G, Domanskyi A, Schober A, Stotz S, et al. Nucleolar disruption in dopaminergic neurons leads to oxidative damage and parkinsonism through repression of mammalian target of rapamycin signaling. J Neurosci. 2011;31:453-60 pubmed publisher
	Wang H, Takenaka K, Nakanishi A, Miki Y. BRCA2 and nucleophosmin coregulate centrosome amplification and form a complex with the Rho effector kinase ROCK2. Cancer Res. 2011;71:68-77 pubmed publisher

product information

Catalog Number :

MAB4500

Subcategory :

Neuroscience

Product Name :

Anti-B23 Antibody, clone 3C9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P06748

Host Name :

Mouse

Antigen :

B23

Clone :

3C9

Conjugate :

Purified

Isotype :

IgM

Product Description :

Anti-B23 Antibody, clone 3C9

Cross Reactivity :

Human

Background :

B23/Nucleophosmin is a major nucleolar phosphoprotein that is mainly located within the nucleolar granular compartment, where preribosomes are assembled. B23/Nucleophosmin is a multifunctional protein that is overexpressed in actively proliferating cells and cancer cells. Antibodies against B23 might be useful for recognition of human peripheral lymphocytes at early stages of their activation for proliferation, preceding the S-phase (Dergunova, 2002). By parallel immunolabeling with the anti-Ki-67 antibody, it was shown that the early changes of B23 amount and localization occurred before an appearance of Ki-67 protein, a well-known marker of proliferating cells (Dergunova, 2002).

ALT Names :

Nucleophosmin

Immunogen :

Total extracts of Daudi cell nuclei.

Specificity :

Recognizes human Nucleophosmin (B23).

Package Size :

100 µg

Uses :

Immunofluorescence;Immunoprecipitation;Western Blotting

Storage :

Maintain at 2-8°C for up 12 months from date of receipt.