OCT-4 [POU5F1] Antibody, clone 7F9.2 | EMD Millipore MAB4419 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

OCT-4 [POU5F1] Antibody, clone 7F9.2

catalog :

MAB4419

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7F9.2

reactivity :

human, mouse

application :

western blot, immunocytochemistry, flow cytometry

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 4	Pijuan Galitó S, Tamm C, Schuster J, Sobol M, Forsberg L, Merry C, et al. Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture. Nat Commun. 2016;7:12170 pubmed publisher
immunocytochemistry; mouse; 1:200; loading ...; fig s1i	Miyawaki S, Kawamura Y, Oiwa Y, Shimizu A, Hachiya T, Bono H, et al. Tumour resistance in induced pluripotent stem cells derived from naked mole-rats. Nat Commun. 2016;7:11471 pubmed publisher
flow cytometry; human; 1:400	Ovchinnikov D, Titmarsh D, Fortuna P, Hidalgo A, Alharbi S, Whitworth D, et al. Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro. Stem Cell Res. 2014;13:251-61 pubmed publisher
immunocytochemistry; mouse; 1:150	Grymula K, Tarnowski M, Piotrowska K, Suszynska M, Mierzejewska K, Borkowska S, et al. Evidence that the population of quiescent bone marrow-residing very small embryonic/epiblast-like stem cells (VSELs) expands in response to neurotoxic treatment. J Cell Mol Med. 2014;18:1797-806 pubmed publisher
immunocytochemistry; mouse	Khromov T, Pantakani D, Nolte J, Wolf M, Dressel R, Engel W, et al. Global and gene-specific histone modification profiles of mouse multipotent adult germline stem cells. Mol Hum Reprod. 2011;17:166-74 pubmed publisher
	Pijuan GalitÃ³ S, Tamm C, AnnerÃ©n C. Serum Inter-Î±-inhibitor activates the Yes tyrosine kinase and YAP/TEAD transcriptional complex in mouse embryonic stem cells. J Biol Chem. 2014;289:33492-502 pubmed publisher
	Han X, Zhao L, Lu G, Ge J, Zhao Y, Zu S, et al. Improving outcomes of acute kidney injury using mouse renal progenitor cells alone or in combination with erythropoietin or suramin. Stem Cell Res Ther. 2013;4:74 pubmed publisher
	Shiozawa S, Kawai K, Okada Y, Tomioka I, Maeda T, Kanda A, et al. Gene targeting and subsequent site-specific transgenesis at the ?-actin (ACTB) locus in common marmoset embryonic stem cells. Stem Cells Dev. 2011;20:1587-99 pubmed publisher
	Armstrong L, Tilgner K, Saretzki G, Atkinson S, Stojkovic M, Moreno R, et al. Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human embryonic stem cells. Stem Cells. 2010;28:661-73 pubmed publisher

product information

Catalog Number :

MAB4419

Subcategory :

Stem Cell Research

Product Name :

Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P20263

Host Name :

Mouse

Antigen :

OCT-4 [POU5F1]

Clone :

7F9.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

Cross Reactivity :

Human;Mouse

Background :

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

ALT Names :

Octamer-binding transcription factor 3;POU class 5 homeobox 1 ;POU domain class 5, transcription factor 1;POU-type homeodomain-containing DNA-binding protein ;octamer-binding transcription factor-3

Immunogen :

Recombinant human Oct-4 protein, a.a. 1-145.

Specificity :

This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.

Package Size :

100 µg

Uses :

Immunocytochemistry;Flow Cytometry;Western Blotting

Storage :

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.