Achaete Scute homolog 2 Antibody, clone 7E2 | EMD Millipore MAB4418 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Achaete Scute homolog 2 Antibody, clone 7E2

catalog :

MAB4418

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7E2

reactivity :

human, mouse

application :

western blot, immunocytochemistry, chromatin immunoprecipitation, western blot knockout validation, immunohistochemistry knockout validation

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 1d western blot knockout validation; mouse; 1:1000; loading ...; fig 2a	Wang C, Wang M, Arrington J, Shan T, Yue F, Nie Y, et al. Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors. Development. 2017;144:235-247 pubmed publisher
chromatin immunoprecipitation; human; fig 4 western blot; human; 1:350; fig 6	Shang Y, Pan Q, Chen L, Ye J, Zhong X, Li X, et al. Achaete scute-like 2 suppresses CDX2 expression and inhibits intestinal neoplastic epithelial cell differentiation. Oncotarget. 2015;6:30993-1006 pubmed publisher
	Tian Y, Pan Q, Shang Y, Zhu R, Ye J, Liu Y, et al. MicroRNA-200 (miR-200) cluster regulation by achaete scute-like 2 (Ascl2): impact on the epithelial-mesenchymal transition in colon cancer cells. J Biol Chem. 2014;289:36101-15 pubmed publisher

product information

Catalog Number :

MAB4418

Subcategory :

Stem Cell Research

Product Name :

Anti-Achaete Scute homolog 2 Antibody, clone 7E2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q99929

Host Name :

Mouse

Antigen :

Achaete Scute homolog 2

Clone :

7E2

Conjugate :

Purified

Isotype :

IgG2bκ

Product Description :

Anti-Achaete Scute homolog 2 Antibody, clone 7E2

Cross Reactivity :

Human

Background :

Achaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).

ALT Names :

achaete-scute complex (Drosophila) homolog-like 2;achaete-scute complex homolog 2 (Drosophila);achaete-scute complex homolog-like 2;achaete-scute complex-like 2;achaete-scute complex-like 2 (Drosophila);mammalian achaete/scute homologue 2

Immunogen :

His-tagged recombinant protein corresponding to human Achaete Scute homolog 2 near the C-terminus.

Specificity :

This antibody recognizes Achaete Scute homolog 2 near the C-terminus.

Package Size :

100 μg

Uses :

Western Blotting;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt