This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Oct-4 Antibody, clone 10H11.2
catalog :
MAB4401
quantity :
100 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10H11.2
reactivity :
human, mouse, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 40
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:250; fig S2A
Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher
  • immunocytochemistry; domestic rabbit; 1:350; fig 3c
Sang C, Cao X, Chen F, Yang X, Zhang Y. Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus. Stem Cells Int. 2016;2016:8283257 pubmed
  • immunocytochemistry; human; 1:500; fig 3b
Lv X, Guo Q, Han F, Chen C, Ling C, Chen W, et al. Electrospun Poly(l-lactide)/Poly(ethylene glycol) Scaffolds Seeded with Human Amniotic Mesenchymal Stem Cells for Urethral Epithelium Repair. Int J Mol Sci. 2016;17: pubmed publisher
  • immunocytochemistry; domestic rabbit; 1:250; fig 1
Zhang L, Chen S, Chang P, Bao N, Yang C, Ti Y, et al. Harmful Effects of Leukocyte-Rich Platelet-Rich Plasma on Rabbit Tendon Stem Cells In Vitro. Am J Sports Med. 2016;44:1941-51 pubmed publisher
  • immunocytochemistry; human; 1:2000; fig 1
De Filippis L, Halikere A, McGowan H, Moore J, Tischfield J, Hart R, et al. Ethanol-mediated activation of the NLRP3 inflammasome in iPS cells and iPS cells-derived neural progenitor cells. Mol Brain. 2016;9:51 pubmed publisher
  • immunocytochemistry; human; fig s1
Zou L, Chen Q, Quanbeck Z, Bechtold J, Kaufman D. Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells. Sci Rep. 2016;6:22868 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 5
Zalis M, Reyes J, Augustsson P, Holmqvist S, Roybon L, Laurell T, et al. Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis. Integr Biol (Camb). 2016;8:332-40 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Pacheco L, Gomez L, Dias J, Ziebarth N, Howard G, Schiller P. Progerin expression disrupts critical adult stem cell functions involved in tissue repair. Aging (Albany NY). 2014;6:1049-63 pubmed
  • flow cytometry; human; 1:400
Turner J, Quek L, Titmarsh D, Krömer J, Kao L, Nielsen L, et al. Metabolic profiling and flux analysis of MEL-2 human embryonic stem cells during exponential growth at physiological and atmospheric oxygen concentrations. PLoS ONE. 2014;9:e112757 pubmed publisher
  • immunocytochemistry; domestic rabbit; 1:500
Liu C, Guo Q, Li J, Wang S, Wang Y, Li B, et al. Identification of rabbit annulus fibrosus-derived stem cells. PLoS ONE. 2014;9:e108239 pubmed publisher
  • immunohistochemistry; human; 1:500; loading ...; fig 2a3
Alvarez A, Field M, Bushnev S, Longo M, Sugaya K. The effects of histone deacetylase inhibitors on glioblastoma-derived stem cells. J Mol Neurosci. 2015;55:7-20 pubmed publisher
  • immunocytochemistry; human; 1:200
Muchkaeva I, Dashinimaev E, Artyuhov A, Myagkova E, Vorotelyak E, Yegorov Y, et al. Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells. Acta Naturae. 2014;6:45-53 pubmed
  • immunocytochemistry; human; 1:500
Radan L, Hughes C, Teichroeb J, Vieira Zamora F, Jewer M, Postovit L, et al. Microenvironmental regulation of telomerase isoforms in human embryonic stem cells. Stem Cells Dev. 2014;23:2046-66 pubmed publisher
  • immunocytochemistry; human; 1:500
Wang L, Zhu H, Wu J, Li N, Hua J. Characterization of embryonic stem-like cells derived from HEK293T cells through miR302/367 expression and their potentiality to differentiate into germ-like cells. Cytotechnology. 2014;66:729-40 pubmed publisher
Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, et al. Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun. 2019;7:208 pubmed publisher
Han Y, Yuan F, Deng C, He F, Zhang Y, Shen H, et al. Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release. Aging (Albany NY). 2019;11:10252-10265 pubmed publisher
Soda M, Saitoh I, Murakami T, Inada E, Iwase Y, Noguchi H, et al. Repeated human deciduous tooth-derived dental pulp cell reprogramming factor transfection yields multipotent intermediate cells with enhanced iPS cell formation capability. Sci Rep. 2019;9:1490 pubmed publisher
Rao L, Qian Y, Khodabukus A, Ribar T, Bursac N. Engineering human pluripotent stem cells into a functional skeletal muscle tissue. Nat Commun. 2018;9:126 pubmed publisher
Tadeu A, Lin S, Hou L, Chung L, Zhong M, Zhao H, et al. Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells. PLoS ONE. 2015;10:e0122493 pubmed publisher
Whitworth D, Frith J, Frith T, Ovchinnikov D, Cooper White J, Wolvetang E. Derivation of mesenchymal stromal cells from canine induced pluripotent stem cells by inhibition of the TGF?/activin signaling pathway. Stem Cells Dev. 2014;23:3021-33 pubmed publisher
Kandasamy K, Narayanan K, Ni M, Du C, Wan A, Zink D. Polysulfone membranes coated with polymerized 3,4-dihydroxy-l-phenylalanine are a versatile and cost-effective synthetic substrate for defined long-term cultures of human pluripotent stem cells. Biomacromolecules. 2014;15:2067-78 pubmed publisher
Xia H, Bodempudi V, Benyumov A, Hergert P, Tank D, Herrera J, et al. Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis. Am J Pathol. 2014;184:1369-83 pubmed publisher
Yin M, Fang Z, Jiang W, Xing F, Jiang M, Kong P, et al. The Oct4 promoter-EGFP transgenic rabbit: a new model for monitoring the pluripotency of rabbit stem cells. Int J Dev Biol. 2013;57:845-52 pubmed publisher
Chen C, Du F, Xu J, Chang W, Liu C, Su H, et al. Synergistic effect of trichostatin A and scriptaid on the development of cloned rabbit embryos. Theriogenology. 2013;79:1284-93 pubmed publisher
Puttonen K, Ruponen M, Kauppinen R, Wojciechowski S, Hovatta O, Koistinaho J. Improved method of producing human neural progenitor cells of high purity and in large quantities from pluripotent stem cells for transplantation studies. Cell Transplant. 2013;22:1753-66 pubmed publisher
Chen C, Xu J, Chang W, Liu C, Su H, Chen Y, et al. Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos. Reprod Biomed Online. 2012;25:358-70 pubmed publisher
Vega S, Liu E, Patel P, Kulesa A, Carlson A, Ma Y, et al. High-content imaging-based screening of microenvironment-induced changes to stem cells. J Biomol Screen. 2012;17:1151-62 pubmed
Chen C, Chang W, Liu C, Su H, Shyue S, Cheng W, et al. Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos. Reprod Biomed Online. 2012;24:433-42 pubmed publisher
Whitworth D, Ovchinnikov D, Wolvetang E. Generation and characterization of LIF-dependent canine induced pluripotent stem cells from adult dermal fibroblasts. Stem Cells Dev. 2012;21:2288-97 pubmed publisher
Pezzolo A, Parodi F, Marimpietri D, Raffaghello L, Cocco C, Pistorio A, et al. Oct-4+/Tenascin C+ neuroblastoma cells serve as progenitors of tumor-derived endothelial cells. Cell Res. 2011;21:1470-86 pubmed publisher
G mez M, Serrano M, Pope C, Jenkins J, Biancardi M, L pez M, et al. Derivation of cat embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells. Theriogenology. 2010;74:498-515 pubmed publisher
Sung L, Chang C, Amano T, Lin C, Amano M, Treaster S, et al. Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes. Cell Reprogram. 2010;12:203-11 pubmed publisher
Moore J, Atze K, Yeung P, Toro Ramos A, Camarillo C, Thompson K, et al. Efficient, high-throughput transfection of human embryonic stem cells. Stem Cell Res Ther. 2010;1:23 pubmed publisher
Zhou Q, Chy H, Laslett A. Preparation of defined human embryonic stem cell populations for transcriptional profiling. Curr Protoc Stem Cell Biol. 2010;Chapter 1:Unit1B.7 pubmed publisher
Wang Y, Meng L, Hu H, Zhang Y, Zhao C, Li Q, et al. Oct-4B isoform is differentially expressed in breast cancer cells: hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription. Biosci Rep. 2011;31:109-15 pubmed publisher
Zhang J, Wang J. Characterization of differential properties of rabbit tendon stem cells and tenocytes. BMC Musculoskelet Disord. 2010;11:10 pubmed publisher
Goff L, Davila J, Swerdel M, Moore J, Cohen R, Wu H, et al. Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors. PLoS ONE. 2009;4:e7192 pubmed publisher
Zuk P. The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used. J Cell Biochem. 2009;106:867-77 pubmed publisher
Bao S, Wu Q, Li Z, Sathornsumetee S, Wang H, McLendon R, et al. Targeting cancer stem cells through L1CAM suppresses glioma growth. Cancer Res. 2008;68:6043-8 pubmed publisher
SHERIDAN S, Gil S, Wilgo M, Pitt A. Microporous membrane growth substrates for embryonic stem cell culture and differentiation. Methods Cell Biol. 2008;86:29-57 pubmed publisher
product information
Catalog Number :
MAB4401
Subcategory :
Stem Cell Research
Product Name :
Anti-Oct-4 Antibody, clone 10H11.2
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q01860
Host Name :
Mouse
Antigen :
Oct 4
Clone :
clone 10H11.2
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-Oct-4 Antibody, clone 10H11.2
Cross Reactivity :
Human
Background :
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
ALT Names :
Octamer-binding transcription factor 3;POU class 5 homeobox 1;POU domain class 5, transcription factor 1;POU domain, class 5, transcription factor 1;POU-type homeodomain-containing DNA-binding protein;octamer-binding transcription factor-3
Immunogen :
Recombinant GST fusion protein, human Oct-4 (aa 1-134).
Specificity :
Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
Package Size :
100 μg
Uses :
Western Blotting;Flow Cytometry;Immunocytochemistry;ELISA
Storage :
Stable for 1 year at from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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