TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J | EMD Millipore MAB4354 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J

catalog :

MAB4354

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TRA-2-54/2J

application :

immunocytochemistry, immunoprecipitation, flow cytometry

citations: 1

Reference
Miki T, Lehmann T, Cai H, Stolz D, Strom S. Stem cell characteristics of amniotic epithelial cells. Stem Cells. 2005;23:1549-59 pubmed

product information

Catalog Number :

MAB4354

Subcategory :

Stem Cell Research

Product Name :

Anti-TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

TRA-2-54

Clone :

TRA-2-54/2J

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J

Cross Reactivity :

Human;Monkey;Baboon;Chimpanzee;Primate

Background :

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Immunogen :

2102Ep human Embryonal Carcinoma Cells

Specificity :

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This antibody does not cross react with other isozymes of AP.

Package Size :

100 µg

Uses :

Flow Cytometry;Immunofluorescence;Immunoprecipitation

Storage :

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.