Oct-4 Antibody, clone 9E3.2 | EMD Millipore MAB4305 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Oct-4 Antibody, clone 9E3.2

catalog :

MAB4305

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9E3.2

reactivity :

human, pigs

application :

western blot, ELISA, immunocytochemistry

citations: 22

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human	Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, et al. Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis. PLoS ONE. 2015;10:e0118670 pubmed publisher
western blot; pigs ; 1:200	Uh K, Park C, Choi K, Park J, Jeong Y, Roh S, et al. Analysis of imprinted IGF2/H19 gene methylation and expression in normal fertilized and parthenogenetic embryonic stem cells of pigs. Anim Reprod Sci. 2014;147:47-55 pubmed publisher
	Byun Y, Tibrewal S, Kim E, Yco L, Sarkar J, Ivanir Y, et al. Keratocytes derived from spheroid culture of corneal stromal cells resemble tissue resident keratocytes. PLoS ONE. 2014;9:e112781 pubmed publisher
	Jung I. Soluble extract from Moringa oleifera leaves with a new anticancer activity. PLoS ONE. 2014;9:e95492 pubmed publisher
	Li D, Isherwood S, Motz A, Zang R, Yang S, Wang J, et al. Cell-based screening of traditional Chinese medicines for proliferation enhancers of mouse embryonic stem cells. Biotechnol Prog. 2013;29:738-44 pubmed publisher
	Suzuki Y, Haraguchi N, Takahashi H, Uemura M, Nishimura J, Hata T, et al. SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers. Int J Oncol. 2013;42:161-7 pubmed publisher
	Zhao Y, Lin J, Wang L, Chen B, Zhou C, Chen T, et al. Derivation and characterization of ovine embryonic stem-like cell lines in semi-defined medium without feeder cells. J Exp Zool A Ecol Genet Physiol. 2011;315:639-48 pubmed publisher
	Vackova I, Novakova Z, Krylov V, Okada K, Kott T, Fulka H, et al. Analysis of marker expression in porcine cell lines derived from blastocysts produced in vitro and in vivo. J Reprod Dev. 2011;57:594-603 pubmed
	Tsai L, Yu C, Chang Y, Yu C, Chou M. Markedly increased Oct4 and Nanog expression correlates with cisplatin resistance in oral squamous cell carcinoma. J Oral Pathol Med. 2011;40:621-8 pubmed publisher
	An G, Miner C, Nixon J, Kincade P, Bryant J, Tucker P, et al. Loss of Bright/ARID3a function promotes developmental plasticity. Stem Cells. 2010;28:1560-7 pubmed publisher
	Shin D, Liu R, Klich I, Wu W, Ratajczak J, Kucia M, et al. Molecular signature of adult bone marrow-purified very small embryonic-like stem cells supports their developmental epiblast/germ line origin. Leukemia. 2010;24:1450-61 pubmed publisher
	Lee P, Lin H, Jiang S, Lu P, Chou K, Fang H, et al. Mouse kidney progenitor cells accelerate renal regeneration and prolong survival after ischemic injury. Stem Cells. 2010;28:573-84 pubmed publisher
	Valli A, Rosner M, Fuchs C, Siegel N, Bishop C, Dolznig H, et al. Embryoid body formation of human amniotic fluid stem cells depends on mTOR. Oncogene. 2010;29:966-77 pubmed publisher
	Yang Y, Wang S, Wu I, Chang C, Huang Y, Lee O, et al. A tumorigenic homeobox (HOX) gene expressing human gastric cell line derived from putative gastric stem cell. Eur J Gastroenterol Hepatol. 2009;21:1016-23 pubmed publisher
	Zuk P. The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used. J Cell Biochem. 2009;106:867-77 pubmed publisher
	Yang Y, Wang S, Hung H, Chang C, Wu I, Huang Y, et al. Isolation and characterization of human gastric cell lines with stem cell phenotypes. J Gastroenterol Hepatol. 2007;22:1460-8 pubmed
	Revazova E, Turovets N, Kochetkova O, Kindarova L, Kuzmichev L, Janus J, et al. Patient-specific stem cell lines derived from human parthenogenetic blastocysts. Cloning Stem Cells. 2007;9:432-49 pubmed
	Cormier J, zur Nieden N, Rancourt D, Kallos M. Expansion of undifferentiated murine embryonic stem cells as aggregates in suspension culture bioreactors. Tissue Eng. 2006;12:3233-45 pubmed
	Jian R, Cheng X, Jiang J, Deng S, Hu F, Zhang J. A cDNA-based random RNA interference library for functional genetic screens in embryonic stem cells. Stem Cells. 2007;25:1904-12 pubmed
	Sidhu K, Lie K, Tuch B. Transgenic human fetal fibroblasts as feeder layer for human embryonic stem cell lineage selection. Stem Cells Dev. 2006;15:741-7 pubmed
	Mitalipova M, Palmarini G. Isolation and characterization of human embryonic stem cells. Methods Mol Biol. 2006;331:55-76 pubmed
	Izadpanah R, Trygg C, Patel B, Kriedt C, Dufour J, Gimble J, et al. Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem. 2006;99:1285-97 pubmed

product information

Catalog Number :

MAB4305

Subcategory :

Stem Cell Research

Product Name :

Anti-Oct-4 Antibody, clone 9E3.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q01860

Host Name :

Mouse

Antigen :

Oct 4

Clone :

9E3.2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Oct-4 Antibody, clone 9E3.2

Cross Reactivity :

Human;Rat;Mouse

Background :

Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

ALT Names :

Octamer-4;Oct-3

Immunogen :

Recombinant protein corresponding to amino acids 143-359 of Human Oct-4

Specificity :

This antibody detects both Oct-4A and Oct4B isoforms. Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. Oct4 expression has been seen in mouse somatic stem cells, including those in the brain (Lengner, C.J, et al., 2007).

Package Size :

100 µg

Uses :

Western Blotting;ELISA

Storage :

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.