PCNA Antibody, clone PC10 | EMD Millipore MAB424 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PCNA Antibody, clone PC10

catalog :

MAB424

quantity :

500 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PC10

The same clone is also sold as:

BioLegend: 307909, 307914, 307902, 307908, 307901, 307904, 307912
LifeSpan Biosciences: LS-C170844, LS-C87459, LS-C358014, LS-B3434, LS-C86889, LS-C87503, LS-C43736, LS-C9535, LS-C87502, LS-B9987
Enzo Life Sciences: ALX-801-091-L001
Cell Signaling Technology: 2586, 8580
OriGene: AM10035PU-N, TA309795, AM10035PU-S
Exbio: 11-182-C100, 1P-182-T100, 1P-182-T025
Invitrogen: 13-3900, 13-3940, MA5-11358
GeneTex: GTX20029
Novus Biologicals: NB500-106, NBP2-80905
Abcam: ab29
Santa Cruz Biotechnology: sc-56 AC, sc-56, sc-56 HRP
Bio-Rad: MCA1558F, MCA1558P, MCA1558

reactivity :

human, mouse, rat, zebrafish

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 53

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:2000; loading ...; fig 4e immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 5c	Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, et al. Modeling medulloblastoma in vivo and with human cerebellar organoids. Nat Commun. 2020;11:583 pubmed publisher
western blot; human; fig 6a	Wilson R, Biasutto A, Wang L, Fischer R, Baple E, Crosby A, et al. PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions. DNA Repair (Amst). 2017;50:22-35 pubmed publisher
immunohistochemistry; rat; 1:50; loading ...; fig 6a	Ibañez Rodriguez M, Noctor S, Muñoz E. Cellular Basis of Pineal Gland Development: Emerging Role of Microglia as Phenotype Regulator. PLoS ONE. 2016;11:e0167063 pubmed publisher
immunohistochemistry - paraffin section; zebrafish ; 1:100; fig 5	He S, Mansour M, Zimmerman M, Ki D, Layden H, Akahane K, et al. Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain. elife. 2016;5: pubmed publisher
immunocytochemistry; human; fig 3 immunocytochemistry; mouse; 1:100; fig 3	Wong F, Fei J, Mora Bermúdez F, Taverna E, Haffner C, Fu J, et al. Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex. PLoS Biol. 2015;13:e1002217 pubmed publisher
immunohistochemistry - free floating section; zebrafish ; 1:500	Edelmann K, Glashauser L, Sprungala S, Hesl B, Fritschle M, Ninkovic J, et al. Increased radial glia quiescence, decreased reactivation upon injury and unaltered neuroblast behavior underlie decreased neurogenesis in the aging zebrafish telencephalon. J Comp Neurol. 2013;521:3099-115 pubmed publisher
western blot; mouse	Kelly C, Thymiakou E, Dixon J, Tanaka S, Godwin J, Episkopou V. Rnf165/Ark2C enhances BMP-Smad signaling to mediate motor axon extension. PLoS Biol. 2013;11:e1001538 pubmed publisher
western blot; mouse; 1:1000	Chen C, Sloane J, Li H, Aytan N, Giannaris E, Zeldich E, et al. The antiaging protein Klotho enhances oligodendrocyte maturation and myelination of the CNS. J Neurosci. 2013;33:1927-39 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Petkau T, Neal S, Orban P, MacDonald J, Hill A, Lu G, et al. Progranulin expression in the developing and adult murine brain. J Comp Neurol. 2010;518:3931-47 pubmed publisher
	Verdile N, Pasquariello R, Scolari M, Scirè G, Brevini T, Gandolfi F. A Detailed Study of Rainbow Trout (Onchorhynchus mykiss) Intestine Revealed That Digestive and Absorptive Functions Are Not Linearly Distributed along Its Length. Animals (Basel). 2020;10: pubmed publisher
	Bonnefont J, Tiberi L, van den Ameele J, Potier D, Gaber Z, Lin X, et al. Cortical Neurogenesis Requires Bcl6-Mediated Transcriptional Repression of Multiple Self-Renewal-Promoting Extrinsic Pathways. Neuron. 2019;103:1096-1108.e4 pubmed publisher
	Huang H, Chen X, Li D, He Y, Li Y, Du Z, et al. Combination of Î±-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells. PLoS ONE. 2015;10:e0144293 pubmed publisher
	Huang H, Cao K, Malik S, Zhang Q, Li D, Chang R, et al. Combination of 12-O-tetradecanoylphorbol-13-acetate with diethyldithiocarbamate markedly inhibits pancreatic cancer cell growth in 3D culture and in immunodeficient mice. Int J Mol Med. 2015;35:1617-24 pubmed publisher
	Kuroda Y, Hayashi S, Hagio S, Abe M, Furukawa S, Nakae D. Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat. J Toxicol Pathol. 2014;27:147-51 pubmed publisher
	Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, et al. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 2014;42:8433-48 pubmed publisher
	Gill R, Michael A, Westley L, Kocher H, Murphy J, Dhoot G. SULF1/SULF2 splice variants differentially regulate pancreatic tumour growth progression. Exp Cell Res. 2014;324:157-71 pubmed publisher
	Litbarg N, Vujicic S, Setty S, Sethupathi P, Dunea G, Arruda J, et al. A novel model of surgical injury in adult rat kidney: a "pouch model". Sci Rep. 2013;3:2890 pubmed publisher
	Chen X, Paudyal S, Chin R, You Z. PCNA promotes processive DNA end resection by Exo1. Nucleic Acids Res. 2013;41:9325-38 pubmed publisher
	Koundrioukoff S, Carignon S, Técher H, Letessier A, Brison O, Debatisse M. Stepwise activation of the ATR signaling pathway upon increasing replication stress impacts fragile site integrity. PLoS Genet. 2013;9:e1003643 pubmed publisher
	Karger C, Kurtz F, Steppan D, Schwarzensteiner I, Machura K, Angel P, et al. Procollagen I-expressing renin cell precursors. Am J Physiol Renal Physiol. 2013;305:F355-61 pubmed publisher
	Pan Y, Wang W, Xia Z. Assessment of adult neurogenesis in mice. Curr Protoc Toxicol. 2013;Chapter 12:Unit12.20 pubmed publisher
	Niwa N, Akimoto Kato A, Sakuma M, Kuraku S, Hayashi S. Homeogenetic inductive mechanism of segmentation in polychaete tail regeneration. Dev Biol. 2013;381:460-70 pubmed publisher
	Akane H, Shiraki A, Imatanaka N, Akahori Y, Itahashi M, Ohishi T, et al. Glycidol induces axonopathy by adult-stage exposure and aberration of hippocampal neurogenesis affecting late-stage differentiation by developmental exposure in rats. Toxicol Sci. 2013;134:140-54 pubmed publisher
	Solarczyk K, Zarebski M, Dobrucki J. Inducing local DNA damage by visible light to study chromatin repair. DNA Repair (Amst). 2012;11:996-1002 pubmed publisher
	Tillhon M, Cazzalini O, Nardo T, Necchi D, Sommatis S, Stivala L, et al. p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG. DNA Repair (Amst). 2012;11:844-52 pubmed publisher
	Beck H, Nähse Kumpf V, Larsen M, O Hanlon K, Patzke S, Holmberg C, et al. Cyclin-dependent kinase suppression by WEE1 kinase protects the genome through control of replication initiation and nucleotide consumption. Mol Cell Biol. 2012;32:4226-36 pubmed
	Lee K, Rhee K. Separase-dependent cleavage of pericentrin B is necessary and sufficient for centriole disengagement during mitosis. Cell Cycle. 2012;11:2476-85 pubmed publisher
	Tichy E, Pillai R, Deng L, Tischfield J, Hexley P, Babcock G, et al. The abundance of Rad51 protein in mouse embryonic stem cells is regulated at multiple levels. Stem Cell Res. 2012;9:124-34 pubmed publisher
	Najbauer J, Huszthy P, Barish M, Garcia E, Metz M, Myers S, et al. Cellular host responses to gliomas. PLoS ONE. 2012;7:e35150 pubmed publisher
	Islam M, Fujinaga R, Yanai A, Jahan M, Takeshita Y, Kokubu K, et al. Characterization of the "sporadically lurking HAP1-immunoreactive (SLH) cells" in the hippocampus, with special reference to the expression of steroid receptors, GABA, and progenitor cell markers. Neuroscience. 2012;210:67-81 pubmed publisher
	Postnikov Y, Kurahashi T, Zhou M, Bustin M. The nucleosome binding protein HMGN1 interacts with PCNA and facilitates its binding to chromatin. Mol Cell Biol. 2012;32:1844-54 pubmed publisher
	Kelava I, Reillo I, Murayama A, Kalinka A, Stenzel D, Tomancak P, et al. Abundant occurrence of basal radial glia in the subventricular zone of embryonic neocortex of a lissencephalic primate, the common marmoset Callithrix jacchus. Cereb Cortex. 2012;22:469-81 pubmed publisher
	Muscolini M, Montagni E, Palermo V, Di Agostino S, Gu W, Abdelmoula Souissi S, et al. The cancer-associated K351N mutation affects the ubiquitination and the translocation to mitochondria of p53 protein. J Biol Chem. 2011;286:39693-702 pubmed publisher
	Su L, Cui H, Xu C, Xie X, Chen Q, Gao X. Putative rabbit conjunctival epithelial stem/progenitor cells preferentially reside in palpebral conjunctiva. Curr Eye Res. 2011;36:797-803 pubmed publisher
	Lin P, Ke Y, Su C, Shiao H, Hsieh H, Chao Y, et al. Inhibition of HIV-1 Tat-mediated transcription by a coumarin derivative, BPRHIV001, through the Akt pathway. J Virol. 2011;85:9114-26 pubmed publisher
	Yang G, Hinson M, Bordner J, Lin Q, Fernando A, La P, et al. Silencing hyperoxia-induced C/EBP? in neonatal mice improves lung architecture via enhanced proliferation of alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2011;301:L187-96 pubmed publisher
	Klemm D, Majka S, Crossno J, Psilas J, Reusch J, Garat C. Reduction of reactive oxygen species prevents hypoxia-induced CREB depletion in pulmonary artery smooth muscle cells. J Cardiovasc Pharmacol. 2011;58:181-91 pubmed publisher
	Pritchard M, Malinak R, Nagy L. Early growth response (EGR)-1 is required for timely cell-cycle entry and progression in hepatocytes after acute carbon tetrachloride exposure in mice. Am J Physiol Gastrointest Liver Physiol. 2011;300:G1124-31 pubmed publisher
	Hayes S, Ahmed S, Clark P. Immunohistochemical assessment for Cdx2 expression in the Barrett metaplasia-dysplasia-adenocarcinoma sequence. J Clin Pathol. 2011;64:110-3 pubmed publisher
	Maurer C, Schonthaler H, Mueller K, Neuhauss S. Distinct retinal deficits in a zebrafish pyruvate dehydrogenase-deficient mutant. J Neurosci. 2010;30:11962-72 pubmed publisher
	Junek A, Rusak B, Semba K. Short-term sleep deprivation may alter the dynamics of hippocampal cell proliferation in adult rats. Neuroscience. 2010;170:1140-52 pubmed publisher
	Gay S, Lachages A, Millot G, Courbet S, Letessier A, Debatisse M, et al. Nucleotide supply, not local histone acetylation, sets replication origin usage in transcribed regions. EMBO Rep. 2010;11:698-704 pubmed publisher
	Bianchi M, Hernando Insúa A, Chasseing N, Rodriguez J, Elias F, Lago N, et al. Oligodeoxynucleotide IMT504 induces a marked recovery in a streptozotocin-induced model of diabetes in rats: correlation with an early increase in the expression of nestin and neurogenin 3 progenitor cell markers. Diabetologia. 2010;53:1184-9 pubmed publisher
	Beck H, Nähse V, Larsen M, Groth P, Clancy T, Lees M, et al. Regulators of cyclin-dependent kinases are crucial for maintaining genome integrity in S phase. J Cell Biol. 2010;188:629-38 pubmed publisher
	Munro S, Khaire N, Inche A, Carr S, La Thangue N. Lysine methylation regulates the pRb tumour suppressor protein. Oncogene. 2010;29:2357-67 pubmed publisher
	Reimer M, Sörensen I, Kuscha V, Frank R, Liu C, Becker C, et al. Motor neuron regeneration in adult zebrafish. J Neurosci. 2008;28:8510-6 pubmed publisher
	Brun J, Chiu R, Lockhart K, Xiao W, Wouters B, Gray D. hMMS2 serves a redundant role in human PCNA polyubiquitination. BMC Mol Biol. 2008;9:24 pubmed publisher
	von Lukowicz T, Hassa P, Lohmann C, Boren J, Braunersreuther V, Mach F, et al. PARP1 is required for adhesion molecule expression in atherogenesis. Cardiovasc Res. 2008;78:158-66 pubmed
	Sargeant T, Day D, Mrkusich E, Foo D, Miller J. Mu opioid receptors are expressed on radial glia but not migrating neuroblasts in the late embryonic mouse brain. Brain Res. 2007;1175:28-38 pubmed
	Ohtaka Maruyama C, Miwa A, Kawano H, Kasai M, Okado H. Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain. J Comp Neurol. 2007;502:1098-108 pubmed
	Fukumoto S, Miner J, Ida H, Fukumoto E, Yuasa K, Miyazaki H, et al. Laminin alpha5 is required for dental epithelium growth and polarity and the development of tooth bud and shape. J Biol Chem. 2006;281:5008-16 pubmed
	Guida T, Salvatore G, Faviana P, Giannini R, Garcia Rostan G, Provitera L, et al. Mitogenic effects of the up-regulation of minichromosome maintenance proteins in anaplastic thyroid carcinoma. J Clin Endocrinol Metab. 2005;90:4703-9 pubmed
	Fukumoto S, Kiba T, Hall B, Iehara N, Nakamura T, Longenecker G, et al. Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts. J Cell Biol. 2004;167:973-83 pubmed

product information

Catalog Number :

MAB424

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-PCNA Antibody, clone PC10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12004

Host Name :

Mouse

Antigen :

PCNA

Clone :

PC10

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-PCNA Antibody, clone PC10

Cross Reactivity :

Invertebrates;Vertebrates

Background :

Proliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells.

ALT Names :

Proliferating Cell Nuclear Antigen; DNA Polymerase delta Processivity Factor

Immunogen :

Rat PCNA made in the protein A vector pR1T2T.

Specificity :

Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.

Package Size :

500 µg

Uses :

ELISA;Flow Cytometry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.