Wilms' Tumor Antibody, NT, clone 6F-H2 | EMD Millipore MAB4234 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Wilms' Tumor Antibody, NT, clone 6F-H2

catalog :

MAB4234

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F-H2

The same clone is also sold as:

LifeSpan Biosciences: LS-C95612, LS-C202917, LS-C141654, LS-C141656, LS-C141658, LS-C141655, LS-C49166, LS-C348626, LS-C312205
Cell Marque: 348M-98
Novus Biologicals: NBP2-81012, NB110-60011
OriGene: TA327754, AM00403SU-L, TA301555
MyBioSource: MBS370117
Abcam: ab212951
Invitrogen: MA1-46028
Abnova: MAB2289, MAB13453, MAB13454
Dako: M3561

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 22

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig 1a immunocytochemistry; human; loading ...; fig 2a,2b	Graziano A, Cardile V, Avola R, Vicario N, Parenti C, Salvatorelli L, et al. Wilms' tumor gene 1 silencing inhibits proliferation of human osteosarcoma MG-63 cell line by cell cycle arrest and apoptosis activation. Oncotarget. 2017;8:13917-13931 pubmed publisher
immunohistochemistry; mouse; fig 1d	Cano E, Carmona R, Ruiz Villalba A, Rojas A, Chau Y, Wagner K, et al. Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A. 2016;113:656-61 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100 western blot; human; 1:1000	Xu C, Wu C, Xia Y, Zhong Z, Liu X, Xu J, et al. WT1 promotes cell proliferation in non-small cell lung cancer cell lines through up-regulating cyclin D1 and p-pRb in vitro and in vivo. PLoS ONE. 2013;8:e68837 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:600 immunohistochemistry - frozen section; rat	Jacobi C, Rudigier L, Scholz H, Kirschner K. Transcriptional regulation by the Wilms tumor protein, Wt1, suggests a role of the metalloproteinase Adamts16 in murine genitourinary development. J Biol Chem. 2013;288:18811-24 pubmed publisher
	Kirschner K, Sciesielski L, Krueger K, Scholz H. Wilms tumor protein-dependent transcription of VEGF receptor 2 and hypoxia regulate expression of the testis-promoting gene Sox9 in murine embryonic gonads. J Biol Chem. 2017;292:20281-20291 pubmed publisher
	Parenti R, Cardile V, Graziano A, Parenti C, Venuti A, Bertuccio M, et al. Wilms' tumor gene 1 (WT1) silencing inhibits proliferation of malignant peripheral nerve sheath tumor sNF96.2 cell line. PLoS ONE. 2014;9:e114333 pubmed publisher
	Wu C, Wang Y, Xia Y, He S, Wang Z, Chen Y, et al. Wilms' tumor 1 enhances Cisplatin-resistance of advanced NSCLC. FEBS Lett. 2014;588:4566-72 pubmed publisher
	Kirschner K, Braun J, Jacobi C, Rudigier L, Persson A, Scholz H. Amine oxidase copper-containing 1 (AOC1) is a downstream target gene of the Wilms tumor protein, WT1, during kidney development. J Biol Chem. 2014;289:24452-62 pubmed publisher
	Kim Y, Mungunsukh O, McCart E, Roehrich P, Yee D, Day R. Mechanism of erythropoietin regulation by angiotensin II. Mol Pharmacol. 2014;85:898-908 pubmed publisher
	Wu C, Zhu W, Qian J, He S, Wu C, Chen Y, et al. WT1 promotes invasion of NSCLC via suppression of CDH1. J Thorac Oncol. 2013;8:1163-9 pubmed publisher
	Vasuri F, Fittipaldi S, Buzzi M, Degiovanni A, Stella A, D Errico Grigioni A, et al. Nestin and WT1 expression in small-sized vasa vasorum from human normal arteries. Histol Histopathol. 2012;27:1195-202 pubmed publisher
	Singh A, Mishra A, Ylaya K, Hewitt S, Sharma K, Saxena S. Wilms tumor-1, claudin-1 and ezrin are useful immunohistochemical markers that help to distinguish schwannoma from fibroblastic meningioma. Pathol Oncol Res. 2012;18:383-9 pubmed publisher
	Sakairi T, Abe Y, Kopp J. TGF-beta1 reduces Wilms' tumor suppressor gene expression in podocytes. Nephrol Dial Transplant. 2011;26:2746-52 pubmed publisher
	Goodfellow S, Rebello M, Toska E, Zeef L, Rudd S, Medler K, et al. WT1 and its transcriptional cofactor BASP1 redirect the differentiation pathway of an established blood cell line. Biochem J. 2011;435:113-25 pubmed publisher
	Sakairi T, Abe Y, Jat P, Kopp J. Cell-cell contact regulates gene expression in CDK4-transformed mouse podocytes. Am J Physiol Renal Physiol. 2010;299:F802-9 pubmed publisher
	Michiels J, Perrin C, Leccia N, Massi D, Grimaldi P, Wagner N. PPARbeta activation inhibits melanoma cell proliferation involving repression of the Wilms' tumour suppressor WT1. Pflugers Arch. 2010;459:689-703 pubmed publisher
	Sakairi T, Abe Y, Kajiyama H, Bartlett L, Howard L, Jat P, et al. Conditionally immortalized human podocyte cell lines established from urine. Am J Physiol Renal Physiol. 2010;298:F557-67 pubmed publisher
	Drossopoulou G, Tsotakos N, Tsilibary E. Impaired transcription factor interplay in addition to advanced glycation end products suppress podocalyxin expression in high glucose-treated human podocytes. Am J Physiol Renal Physiol. 2009;297:F594-603 pubmed publisher
	Weber G, Karbach J, Kuci S, Kreyenberg H, Willasch A, Koscielniak E, et al. WT1 peptide-specific T cells generated from peripheral blood of healthy donors: possible implications for adoptive immunotherapy after allogeneic stem cell transplantation. Leukemia. 2009;23:1634-42 pubmed publisher
	Wagner N, Wagner K, Afanetti M, Nevo F, Antignac C, Michiels J, et al. A novel Wilms' tumor 1 gene mutation in a child with severe renal dysfunction and persistent renal blastema. Pediatr Nephrol. 2008;23:1445-53 pubmed publisher
	Tatsumi N, Oji Y, Tsuji N, Tsuda A, Higashio M, Aoyagi S, et al. Wilms' tumor gene WT1-shRNA as a potent apoptosis-inducing agent for solid tumors. Int J Oncol. 2008;32:701-11 pubmed
	Wagner N, Wagner K, Hammes A, Kirschner K, Vidal V, Schedl A, et al. A splice variant of the Wilms' tumour suppressor Wt1 is required for normal development of the olfactory system. Development. 2005;132:1327-36 pubmed

product information

Catalog Number :

MAB4234

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Wilms' Tumor Antibody, NT, clone 6F-H2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P19544

Host Name :

Mouse

Antigen :

Wilms' Tumor

Clone :

6F-H2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Wilms' Tumor Antibody, NT, clone 6F-H2

Cross Reactivity :

Human

Background :

Wilm's Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilms' tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma.

ALT Names :

WT1

Immunogen :

aa 1-173 amino acides of the WT-1 protein

Specificity :

The antibody reacts with all isoforms of the full-length WT1 protein. The antibody staining location is nuclear.

Package Size :

100 µg

Uses :

ELISA;Immunofluorescence;Western Blotting

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.