Ki-67 Antibody, clone Ki-S5 | EMD Millipore MAB4190 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Ki-67 Antibody, clone Ki-S5

catalog :

MAB4190

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ki-S5

The same clone is also sold as:

LifeSpan Biosciences: LS-B6433

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 26

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:400; fig 5a	Rocktäschel P, Sen A, Cader M. High glucose concentrations mask cellular phenotypes in a stem cell model of tuberous sclerosis complex. Epilepsy Behav. 2019;101:106581 pubmed publisher
immunocytochemistry; human; 1:200; loading ...; tbl s4	Hansen S, Stummann T, Borland H, Hasholt L, Tumer Z, Nielsen J, et al. Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3. Stem Cell Res. 2016;17:306-317 pubmed publisher
immunohistochemistry; rat; 1:250; fig s3	Doobin D, Kemal S, Dantas T, Vallee R. Severe NDE1-mediated microcephaly results from neural progenitor cell cycle arrests at multiple specific stages. Nat Commun. 2016;7:12551 pubmed publisher
immunocytochemistry; human	Rolland M, Li X, Sellier Y, Martin H, Pérez Berezo T, Rauwel B, et al. PPARγ Is Activated during Congenital Cytomegalovirus Infection and Inhibits Neuronogenesis from Human Neural Stem Cells. PLoS Pathog. 2016;12:e1005547 pubmed publisher
flow cytometry; human; fig 2	Laustriat D, Gide J, Barrault L, Chautard E, Benoit C, Auboeuf D, et al. In Vitro and In Vivo Modulation of Alternative Splicing by the Biguanide Metformin. Mol Ther Nucleic Acids. 2015;4:e262 pubmed publisher
immunohistochemistry; human; 1:200	LÃ¶nnqvist S, Rakar J, Briheim K, Kratz G. Biodegradable Gelatin Microcarriers Facilitate Re-Epithelialization of Human Cutaneous Wounds - An In Vitro Study in Human Skin. PLoS ONE. 2015;10:e0128093 pubmed publisher
immunocytochemistry; human; 1:200; fig 2	Karbalaie K, Tanhaei S, Rabiei F, Kiani Esfahani A, Masoudi N, Nasr Esfahani M, et al. Stem cells from human exfoliated deciduous tooth exhibit stromal-derived inducing activity and lead to generation of neural crest cells from human embryonic stem cells. Cell J. 2015;17:37-48 pubmed
immunocytochemistry; human; 1:1000; fig 10	Maury Y, CÃ´me J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:200	Petrakova O, Terskikh V, Chernioglo E, Ashapkin V, Bragin E, Shtratnikova V, et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus. 2014;3:183 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Biadasiewicz K, Fock V, Dekan S, Proestling K, Velicky P, Haider S, et al. Extravillous trophoblast-associated ADAM12 exerts pro-invasive properties, including induction of integrin beta 1-mediated cellular spreading. Biol Reprod. 2014;90:101 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Herwig M, Bergstrom C, Wells J, Höller T, Grossniklaus H. M2/M1 ratio of tumor associated macrophages and PPAR-gamma expression in uveal melanomas with class 1 and class 2 molecular profiles. Exp Eye Res. 2013;107:52-8 pubmed publisher
	Novaes Gomes F, Fernandes J, Vannucci Campos D, Cassilhas R, Viana G, D Almeida V, et al. The beneficial effects of strength exercise on hippocampal cell proliferation and apoptotic signaling is impaired by anabolic androgenic steroids. Psychoneuroendocrinology. 2014;50:106-17 pubmed publisher
	Haider S, Meinhardt G, Velicky P, Otti G, Whitley G, Fiala C, et al. Notch signaling plays a critical role in motility and differentiation of human first-trimester cytotrophoblasts. Endocrinology. 2014;155:263-74 pubmed publisher
	Khalfaoui T, Groulx J, Sabra G, Guezguez A, Basora N, Vermette P, et al. Laminin receptor 37/67LR regulates adhesion and proliferation of normal human intestinal epithelial cells. PLoS ONE. 2013;8:e74337 pubmed publisher
	Kallenbach Thieltges A, Großerüschkamp F, Mosig A, Diem M, Tannapfel A, Gerwert K. Immunohistochemistry, histopathology and infrared spectral histopathology of colon cancer tissue sections. J Biophotonics. 2013;6:88-100 pubmed publisher
	Gagliardi P, di Blasio L, Orso F, Seano G, Sessa R, Taverna D, et al. 3-phosphoinositide-dependent kinase 1 controls breast tumor growth in a kinase-dependent but Akt-independent manner. Neoplasia. 2012;14:719-31 pubmed
	Ng F, Toh T, Ting E, Koh G, Sandanaraj E, Phong M, et al. Progenitor-like traits contribute to patient survival and prognosis in oligodendroglial tumors. Clin Cancer Res. 2012;18:4122-35 pubmed
	Bobustuc G, Smith J, Maddipatla S, Jeudy S, Limaye A, Isley B, et al. MGMT inhibition restores ER? functional sensitivity to antiestrogen therapy. Mol Med. 2012;18:913-29 pubmed publisher
	Menard D, Tremblay E, Ferretti E, Babakissa C, Perron N, Seidman E, et al. Anti-inflammatory effects of epidermal growth factor on the immature human intestine. Physiol Genomics. 2012;44:268-80 pubmed publisher
	Sarkar T, Sharan S, Wang J, Pawar S, Cantwell C, Johnson P, et al. Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPÎ´ expression and contributes to transformation of breast tumor cells. Mol Cell Biol. 2012;32:320-32 pubmed publisher
	Varga I, Hutóczki G, Szemcsák C, Zahuczky G, Toth J, Adamecz Z, et al. Brevican, neurocan, tenascin-C and versican are mainly responsible for the invasiveness of low-grade astrocytoma. Pathol Oncol Res. 2012;18:413-20 pubmed publisher
	Niapour A, Karamali F, Nemati S, Taghipour Z, Mardani M, Nasr Esfahani M, et al. Cotransplantation of human embryonic stem cell-derived neural progenitors and schwann cells in a rat spinal cord contusion injury model elicits a distinct neurogenesis and functional recovery. Cell Transplant. 2012;21:827-43 pubmed publisher
	Smith J, Colon J, Madero Visbal R, Isley B, Konduri S, Baker C. Blockade of MUC1 expression by glycerol guaiacolate inhibits proliferation of human breast cancer cells. Anticancer Agents Med Chem. 2010;10:644-6650 pubmed
	Zhang Y, Xing S, Zhang J, Li J, Li C, Pei Z, et al. Reduction of ?-amyloid deposits by ?-secretase inhibitor is associated with the attenuation of secondary damage in the ipsilateral thalamus and sensory functional improvement after focal cortical infarction in hypertensive rats. J Cereb Blood Flow Metab. 2011;31:572-9 pubmed publisher
	Konduri S, Ticku J, Bobustuc G, Sutphin R, Colon J, Isley B, et al. Blockade of MGMT expression by O6 benzyl guanine leads to inhibition of pancreatic cancer growth and induction of apoptosis. Clin Cancer Res. 2009;15:6087-95 pubmed publisher
	Rittie L, Varani J, Kang S, Voorhees J, Fisher G. Retinoid-induced epidermal hyperplasia is mediated by epidermal growth factor receptor activation via specific induction of its ligands heparin-binding EGF and amphiregulin in human skin in vivo. J Invest Dermatol. 2006;126:732-9 pubmed

product information

Catalog Number :

MAB4190

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Ki-67 Antibody, clone Ki-S5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P46013

Host Name :

Mouse

Antigen :

Ki-67

Clone :

Ki-S5

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Ki-67 Antibody, clone Ki-S5

Cross Reactivity :

Human

Background :

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer.

Immunogen :

Nuclear protein preparation from the human cell line U937.50.

Specificity :

In Immunoprecipitation and Western blot experiments the Ki-S5 antibody recognizes a cell-cycle-associated protein of 345 kD and 395 kD (Kreipe et al., 1993) identical with the Ki-67 antigen. The antibody binds to a formalin-resistant epitope of the Ki-67 antigen. The immunoreactivity of Ki-S5 is confined to the nuclei of proliferating cells and no crossreactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval (Mauri et al., 1984). A comparison of immunohistochemical labeling of fresh and fixed tissue samples of NHL showed that identical results were obtained with Ki-67 and Ki-S5 (Kreipe et al., 1993). The Ki-67 antigen is preferentially expressed during late G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack the Ki-67 antigen. In addition, constantly proliferating cells (e.g. cell lines) react positively to Ki-S5 during the entire cell-cycle. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer.

Package Size :

100 μg

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 1 year at 2-8ºC from date of receipt.