P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 | EMD Millipore MAB4092 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

catalog :

MAB4092

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

KPL-1

The same clone is also sold as:

BioLegend: 328813, 328818, 328807, 328816, 328820, 328806, 328811, 328805, 328821, 328808, 328802, 328815, 328809, 328823, 328817, 328814, 328812, 328819, 328810
LifeSpan Biosciences: LS-B11572
Santa Cruz Biotechnology: sc-13535 L, sc-13535
BD Biosciences: 556055, 556052, 556053

reactivity :

human, bovine

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 3

Reference
Banks J, Herman C, Bailey R. Bromelain decreases neutrophil interactions with P-selectin, but not E-selectin, in vitro by proteolytic cleavage of P-selectin glycoprotein ligand-1. PLoS ONE. 2013;8:e78988 pubmed publisher
Lin H, Yang Y, Yu S, Hsiao K, Liu C, Sia C, et al. Caveolar endocytosis is required for human PSGL-1-mediated enterovirus 71 infection. J Virol. 2013;87:9064-76 pubmed publisher
Azab A, Quang P, Azab F, Pitsillides C, Thompson B, Chonghaile T, et al. P-selectin glycoprotein ligand regulates the interaction of multiple myeloma cells with the bone marrow microenvironment. Blood. 2012;119:1468-78 pubmed publisher

product information

Catalog Number :

MAB4092

Subcategory :

Inflammation & Immunology

Product Name :

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q14242

Host Name :

Mouse

Antigen :

P-Selectin Glycoprotein Ligand-1

Clone :

KPL-1

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

Cross Reactivity :

Human

Background :

Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.

ALT Names :

PSGL-1; CD162

Immunogen :

The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).

Specificity :

P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.

Package Size :

100 µg

Uses :

Flow Cytometry;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Maintain at 2-8°C in undiluted aliquots up to 12 months.