ATM phosphoSer1981 Antibody, clone 10H11.E12 | EMD Millipore MAB3806 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ATM phosphoSer1981 Antibody, clone 10H11.E12

catalog :

MAB3806

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

10H11.E12

The same clone is also sold as:

LifeSpan Biosciences: LS-C180223, LS-C180222, LS-C180224, LS-C180288, LS-C2763
Santa Cruz Biotechnology: sc-47739
OriGene: TA309577
BioLegend: 651204, 651201, 651202, 651203
Abcam: ab36810
Novus Biologicals: NBP2-80586, NB100-306
Rockland Immunochemicals: 200-301-400, 200-303-400, 200-306-400
Invitrogen: 50-9046-41, MA1-46069, 14-9046-37, 14-9046-82, 50-9046-42, 14-9046-80
Cell Signaling Technology: 4526
Abnova: MAB2401

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; loading ...; fig s9a	Natale F, Rapp A, Yu W, Maiser A, Harz H, Scholl A, et al. Identification of the elementary structural units of the DNA damage response. Nat Commun. 2017;8:15760 pubmed publisher
western blot; human; loading ...; fig 4b	Salzano M, Sanz GarcÃa M, Monsalve D, Moura D, Lazo P. VRK1 chromatin kinase phosphorylates H2AX and is required for foci formation induced by DNA damage. Epigenetics. 2015;10:373-83 pubmed publisher
western blot; human	Nakahara T, Tanaka K, Ohno S, Egawa N, Yugawa T, Kiyono T. Activation of NF-ÎºB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1. J Virol. 2015;89:5040-59 pubmed publisher
immunocytochemistry; human; 1:100	Ferlazzo M, Sonzogni L, Granzotto A, Bodgi L, Lartin O, Devic C, et al. Mutations of the Huntington's disease protein impact on the ATM-dependent signaling and repair pathways of the radiation-induced DNA double-strand breaks: corrective effect of statins and bisphosphonates. Mol Neurobiol. 2014;49:1200-11 pubmed publisher
western blot; human; 1:1000	Chen Y, Kamili A, Hardy J, Groblewski G, Khanna K, Byrne J. Tumor protein D52 represents a negative regulator of ATM protein levels. Cell Cycle. 2013;12:3083-97 pubmed publisher
western blot; mouse	Brochier C, Dennis G, Rivieccio M, McLaughlin K, Coppola G, Ratan R, et al. Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons. J Neurosci. 2013;33:8621-32 pubmed publisher
western blot; mouse	Lin R, Ho C, Liu L, Lyu Y. Topoisomerase II? deficiency enhances camptothecin-induced apoptosis. J Biol Chem. 2013;288:7182-92 pubmed publisher
immunocytochemistry; human	Yan Q, Xu R, Zhu L, Cheng X, Wang Z, Manis J, et al. BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8. Mol Cell Biol. 2013;33:845-57 pubmed publisher
immunocytochemistry; human	Solarczyk K, Zarebski M, Dobrucki J. Inducing local DNA damage by visible light to study chromatin repair. DNA Repair (Amst). 2012;11:996-1002 pubmed publisher
	Mezzadra R, de Bruijn M, Jae L, Gomez Eerland R, Duursma A, Scheeren F, et al. SLFN11 can sensitize tumor cells towards IFN-γ-mediated T cell killing. PLoS ONE. 2019;14:e0212053 pubmed publisher
	Chen M, Hsu W, Hsu S, Yang Y, Chuang T, Lin W, et al. PP4 deficiency leads to DNA replication stress that impairs immunoglobulin class switch efficiency. Cell Death Differ. 2019;26:1221-1234 pubmed publisher
	Durant S, Zheng L, Wang Y, Chen K, Zhang L, Zhang T, et al. The brain-penetrant clinical ATM inhibitor AZD1390 radiosensitizes and improves survival of preclinical brain tumor models. Sci Adv. 2018;4:eaat1719 pubmed publisher
	Pustovalova M, Astrelina Т, Grekhova A, Vorobyeva N, Tsvetkova A, Blokhina T, et al. Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells. Aging (Albany NY). 2017;9:2397-2410 pubmed publisher
	Pustovalova M, Grekhova A, Astrelina Т, Nikitina V, Dobrovolskaya E, Suchkova Y, et al. Accumulation of spontaneous ?H2AX foci in long-term cultured mesenchymal stromal cells. Aging (Albany NY). 2016;8:3498-3506 pubmed publisher
	Parplys A, Zhao W, Sharma N, Groesser T, Liang F, Maranon D, et al. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Res. 2015;43:9817-34 pubmed publisher
	Staquicini F, Qian M, Salameh A, Dobroff A, Edwards J, Cimino D, et al. Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. J Biol Chem. 2015;290:7345-59 pubmed publisher
	Schmidt L, Wiedner M, Velimezi G, Prochazkova J, Owusu M, Bauer S, et al. ATMIN is required for the ATM-mediated signaling and recruitment of 53BP1 to DNA damage sites upon replication stress. DNA Repair (Amst). 2014;24:122-130 pubmed publisher
	Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, et al. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. J Immunol. 2014;193:950-60 pubmed publisher
	Khongkow P, Karunarathna U, Khongkow M, Gong C, Gomes A, Yague E, et al. FOXM1 targets NBS1 to regulate DNA damage-induced senescence and epirubicin resistance. Oncogene. 2014;33:4144-55 pubmed publisher
	Zhang T, Penicud K, Bruhn C, Loizou J, Kanu N, Wang Z, et al. Competition between NBS1 and ATMIN controls ATM signaling pathway choice. Cell Rep. 2012;2:1498-504 pubmed publisher
	Jullien L, Mestre M, Roux P, Gire V. Eroded human telomeres are more prone to remain uncapped and to trigger a G2 checkpoint response. Nucleic Acids Res. 2013;41:900-11 pubmed publisher
	Conradt L, Henrich A, Wirth M, Reichert M, Lesina M, Algül H, et al. Mdm2 inhibitors synergize with topoisomerase II inhibitors to induce p53-independent pancreatic cancer cell death. Int J Cancer. 2013;132:2248-57 pubmed publisher
	Hasham M, Snow K, Donghia N, Branca J, Lessard M, Stavnezer J, et al. Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase. J Immunol. 2012;189:2374-82 pubmed publisher
	Bardelle C, Boros J. Development of a high-content high-throughput screening assay for the discovery of ATM signaling inhibitors. J Biomol Screen. 2012;17:912-20 pubmed publisher
	Duxin J, Moore H, Sidorova J, Karanja K, Honaker Y, Dao B, et al. Okazaki fragment processing-independent role for human Dna2 enzyme during DNA replication. J Biol Chem. 2012;287:21980-91 pubmed publisher
	Ondovcik S, Tamblyn L, McPherson J, Wells P. Oxoguanine glycosylase 1 (OGG1) protects cells from DNA double-strand break damage following methylmercury (MeHg) exposure. Toxicol Sci. 2012;128:272-83 pubmed publisher
	Pampeno C, Hurtado A, Meruelo D. ATM kinase is activated by sindbis viral vector infection. Virus Res. 2012;166:97-102 pubmed publisher
	Shouse G, Nobumori Y, Panowicz M, Liu X. ATM-mediated phosphorylation activates the tumor-suppressive function of B56?-PP2A. Oncogene. 2011;30:3755-65 pubmed publisher
	Lee H, Trott J, Haque S, McCormick S, Chiorazzi N, Mongini P. A cyclooxygenase-2/prostaglandin E2 pathway augments activation-induced cytosine deaminase expression within replicating human B cells. J Immunol. 2010;185:5300-14 pubmed publisher
	Kanu N, Penicud K, Hristova M, Wong B, Irvine E, Plattner F, et al. The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain. J Biol Chem. 2010;285:38534-42 pubmed publisher

product information

Catalog Number :

MAB3806

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-ATM phosphoSer1981 Antibody, clone 10H11.E12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q13315

Host Name :

Mouse

Antigen :

ATM phosphoSer1981

Clone :

10H11.E12

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-ATM phosphoSer1981 Antibody, clone 10H11.E12

Cross Reactivity :

Human;Mouse

Background :

ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). Ataxia telangiectasia (AT) is a debilitating neurodegenerative disease that occurs early in childhood, resulting in ataxic movements and speech defects caused by cerebellar degeneration. The underlying cause of the disease is a biochemical dysfunction in the cellular response to specific types of DNA damage, correlated with mutations in the protein kinase ATM (Ataxia telangiectasia mutated). ATM not only is key in the signaling cascade that responds to DNA double-stranded breaks, but it also controls cell-cycle checkpoints and is therefore important in cancer prevention.

ALT Names :

Ataxia Telangiectasia Mutated

Immunogen :

Synthetic peptide corresponding to amino acids 1974-1988 of human ATM (SLAFEEG[pS]QSTTISS).

Specificity :

Reacts with ATM Kinase phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.

Package Size :

100 µg

Uses :

Immunoprecipitation;Western Blotting;Immunocytochemistry

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. This antibody and certain aspects of its use are disclosed and claimed in pending U.S. Patent Applications published as U.S. Patent Publication Nos. 2003/0077661 and 2003/0157572.