NeuN Antibody, clone A60, Cy3 Conjugate | EMD Millipore MAB377C3 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NeuN Antibody, clone A60, Cy3 Conjugate

catalog :

MAB377C3

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

Cy3

clone name :

A60

The same clone is also sold as:

OriGene: AM10122SU-N

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100; loading ...; fig 4c	Brown K, Selfridge J, Lagger S, Connelly J, De Sousa D, Kerr A, et al. The molecular basis of variable phenotypic severity among common missense mutations causing Rett syndrome. Hum Mol Genet. 2016;25:558-70 pubmed publisher
	Hagelkruys A, Lagger S, Krahmer J, Leopoldi A, Artaker M, Pusch O, et al. A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain development in the absence of its paralog. Development. 2014;141:604-616 pubmed publisher

product information

Catalog Number :

MAB377C3

Subcategory :

Neuroscience

Product Name :

Anti-NeuN Antibody, clone A60, Cy3 Conjugate

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

NeuN

Clone :

A60

Conjugate :

Cy3

Isotype :

IgG1

Product Description :

Anti-NeuN Antibody, clone A60, Cy3 Conjugate

Cross Reactivity :

Rat;Mouse

Background :

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

ALT Names :

NEUronal Nuclei;Neuronal Nuclei

Immunogen :

Purified cell nuclei from mouse brain.

Specificity :

NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia.

Package Size :

100 µL

Uses :

Immunocytochemistry

Storage :

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.