NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate | EMD Millipore MAB377A5 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate

catalog :

MAB377A5

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

Alexa Fluor 555

clone name :

A60

The same clone is also sold as:

OriGene: AM10122SU-N

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:2000; fig 7b	Hayashi Y, Morinaga S, Zhang J, Satoh Y, Meredith A, Nakata T, et al. BK channels in microglia are required for morphine-induced hyperalgesia. Nat Commun. 2016;7:11697 pubmed publisher
immunohistochemistry - frozen section; rat; 1:500	Topper L, Baculis B, Valenzuela C. Exposure of neonatal rats to alcohol has differential effects on neuroinflammation and neuronal survival in the cerebellum and hippocampus. J Neuroinflammation. 2015;12:160 pubmed publisher
immunohistochemistry; rat; 1:100	Simpson M, Venkatesh I, Callif B, Thiel L, Coley D, Winsor K, et al. The tumor suppressor HHEX inhibits axon growth when prematurely expressed in developing central nervous system neurons. Mol Cell Neurosci. 2015;68:272-83 pubmed publisher
	Wang Z, Mehra V, Simpson M, Maunze B, Chakraborty A, Holan L, et al. KLF6 and STAT3 co-occupy regulatory DNA and functionally synergize to promote axon growth in CNS neurons. Sci Rep. 2018;8:12565 pubmed publisher
	Ito Ishida A, Yamalanchili H, Shao Y, BAKER S, Heckman L, Lavery L, et al. Genome-wide distribution of linker histone H1.0 is independent of MeCP2. Nat Neurosci. 2018;21:794-798 pubmed publisher
	Loewen J, Barker Haliski M, Dahle E, White H, Wilcox K. Neuronal Injury, Gliosis, and Glial Proliferation in Two Models of Temporal Lobe Epilepsy. J Neuropathol Exp Neurol. 2016;75:366-78 pubmed publisher
	Fowler A, Thompson J, Chen L, Dagda M, Dertien J, Dossou K, et al. Differential sensitivity of prefrontal cortex and hippocampus to alcohol-induced toxicity. PLoS ONE. 2014;9:e106945 pubmed publisher
	Fowler A, Hewetson A, Agrawal R, Dagda M, Dagda R, Moaddel R, et al. Alcohol-induced one-carbon metabolism impairment promotes dysfunction of DNA base excision repair in adult brain. J Biol Chem. 2012;287:43533-42 pubmed publisher

product information

Catalog Number :

MAB377A5

Subcategory :

Neuroscience

Product Name :

Anti-NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

NeuN

Clone :

A60

Conjugate :

AlexaFluor®555

Isotype :

IgG1

Product Description :

Anti-NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate

Cross Reactivity :

Rat;Mouse

Background :

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

ALT Names :

NEUronal Nuclei;Neuronal Nuclei

Immunogen :

Purified cell nuclei from mouse brain.

Specificity :

NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia.

Package Size :

100 µL

Uses :

Immunocytochemistry;Immunohistochemistry

Storage :

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.