MDM2 Antibody, clone SMP14 | EMD Millipore MAB3776 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MDM2 Antibody, clone SMP14

catalog :

MAB3776

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SMP14

The same clone is also sold as:

LifeSpan Biosciences: LS-B163, LS-C179694
BD Biosciences: 556353
MyBioSource: MBS215152
Bio-Rad: MCA1709T, MCA1709
Santa Cruz Biotechnology: sc-965, sc-965 X, sc-965 AC, sc-965 HRP
OriGene: AM00224PU-T, AM00224PU-N, AM00224SU-N
MilliporeSigma: M4308
Abnova: MAB12510
Novus Biologicals: NBP2-80845, NB100-2736

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 15

Reference
Wu H, Leng R. MDM2 mediates p73 ubiquitination: a new molecular mechanism for suppression of p73 function. Oncotarget. 2015;6:21479-92 pubmed
Wang S, Sun W, Zhao Y, McEachern D, Meaux I, BarriÃ¨re C, et al. SAR405838: an optimized inhibitor of MDM2-p53 interaction that induces complete and durable tumor regression. Cancer Res. 2014;74:5855-65 pubmed publisher
Dolfi S, Jäger A, Medina D, Haffty B, Yang J, Hirshfield K. Fulvestrant treatment alters MDM2 protein turnover and sensitivity of human breast carcinoma cells to chemotherapeutic drugs. Cancer Lett. 2014;350:52-60 pubmed publisher
Höglinger G, Arias Carrión O, Ipach B, Oertel W. Origin of the dopaminergic innervation of adult neurogenic areas. J Comp Neurol. 2014;522:2336-48 pubmed publisher
May L, Kramarenko I, Brandon C, Voelkel Johnson C, Roy S, Truong K, et al. Inner ear supporting cells protect hair cells by secreting HSP70. J Clin Invest. 2013;123:3577-87 pubmed publisher
Singh S, Ramamoorthy M, Vaughan C, Yeudall W, Deb S, Palit Deb S. Human oncoprotein MDM2 activates the Akt signaling pathway through an interaction with the repressor element-1 silencing transcription factor conferring a survival advantage to cancer cells. Cell Death Differ. 2013;20:558-66 pubmed publisher
Bhattacharya S, Chaum E, Johnson D, Johnson L. Age-related susceptibility to apoptosis in human retinal pigment epithelial cells is triggered by disruption of p53-Mdm2 association. Invest Ophthalmol Vis Sci. 2012;53:8350-66 pubmed publisher
Conradt L, Henrich A, Wirth M, Reichert M, Lesina M, Algül H, et al. Mdm2 inhibitors synergize with topoisomerase II inhibitors to induce p53-independent pancreatic cancer cell death. Int J Cancer. 2013;132:2248-57 pubmed publisher
Huart A, MacLaine N, Narayan V, Hupp T. Exploiting the MDM2-CK1? protein-protein interface to develop novel biologics that induce UBL-kinase-modification and inhibit cell growth. PLoS ONE. 2012;7:e43391 pubmed publisher
Dolečková I, Rárová L, Gruz J, Vondrusová M, Strnad M, Krystof V. Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves. Fitoterapia. 2012;83:1000-7 pubmed publisher
Adeyemi R, Pintel D. Replication of minute virus of mice in murine cells is facilitated by virally induced depletion of p21. J Virol. 2012;86:8328-32 pubmed publisher
Moran D, Maki C. Nutlin-3a induces cytoskeletal rearrangement and inhibits the migration and invasion capacity of p53 wild-type cancer cells. Mol Cancer Ther. 2010;9:895-905 pubmed publisher
Salem M, Shalbaf M, Gibbons N, Chavan B, Thornton J, Schallreuter K. Enhanced DNA binding capacity on up-regulated epidermal wild-type p53 in vitiligo by H2O2-mediated oxidation: a possible repair mechanism for DNA damage. FASEB J. 2009;23:3790-807 pubmed publisher
Gatz S, Keimling M, Baumann C, Dork T, Debatin K, Fulda S, et al. Resveratrol modulates DNA double-strand break repair pathways in an ATM/ATR-p53- and -Nbs1-dependent manner. Carcinogenesis. 2008;29:519-27 pubmed publisher
Rizos H, Woodruff S, Kefford R. p14ARF interacts with the SUMO-conjugating enzyme Ubc9 and promotes the sumoylation of its binding partners. Cell Cycle. 2005;4:597-603 pubmed

product information

Catalog Number :

MAB3776

Subcategory :

Protein Trafficking

Product Name :

Anti-MDM2 Antibody, clone SMP14

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q00987

Host Name :

Mouse

Antigen :

MDM2

Clone :

SMP14

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-MDM2 Antibody, clone SMP14

Cross Reactivity :

Human;Mouse;Rat

Background :

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

ALT Names :

Murine Double Minute 2

Immunogen :

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

Specificity :

Recognizes human MDM2.

Package Size :

100 µg

Uses :

Immunoprecipitation;Immunohistochemistry;Immunohistochemistry (Paraffin)

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.