VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9 | EMD Millipore MAB3757 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9

catalog :

MAB3757

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9D9F9

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig 1c western blot; human; loading ...; fig 4e	Olmeda D, Cerezo Wallis D, Riveiro Falkenbach E, Pennacchi P, Contreras Alcalde M, Ibarz N, et al. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine. Nature. 2017;546:676-680 pubmed publisher
western blot; human; 1:500; fig 2	Gschweitl M, Ulbricht A, Barnes C, Enchev R, Stoffel Studer I, Meyer Schaller N, et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. elife. 2016;5:e13841 pubmed publisher
western blot; human	Stewart J, Ma X, Megison M, Nabers H, Cance W, Kurenova E, et al. Inhibition of FAK and VEGFR-3 binding decreases tumorigenicity in neuroblastoma. Mol Carcinog. 2015;54:9-23 pubmed publisher
western blot; human; fig 7	Beierle E, Ma X, Stewart J, Megison M, Cance W, Kurenova E. Inhibition of the focal adhesion kinase and vascular endothelial growth factor receptor-3 interaction leads to decreased survival in human neuroblastoma cell lines. Mol Carcinog. 2014;53:230-42 pubmed publisher
	Durré T, Morfoisse F, Erpicum C, Ebroin M, Blacher S, García Caballero M, et al. uPARAP/Endo180 receptor is a gatekeeper of VEGFR-2/VEGFR-3 heterodimerisation during pathological lymphangiogenesis. Nat Commun. 2018;9:5178 pubmed publisher
	Schmieder R, Hoffmann J, Becker M, Bhargava A, Müller T, Kahmann N, et al. Regorafenib (BAY 73-4506): antitumor and antimetastatic activities in preclinical models of colorectal cancer. Int J Cancer. 2014;135:1487-96 pubmed publisher
	Marti L, Pavon L, Severino P, Sibov T, Guilhen D, Moreira Filho C. Vascular endothelial growth factor-A enhances indoleamine 2,3-dioxygenase expression by dendritic cells and subsequently impacts lymphocyte proliferation. Mem Inst Oswaldo Cruz. 2014;109:70-9 pubmed publisher
	Iwasaki T, Takeda Y, Maruyama K, Yokosaki Y, Tsujino K, Tetsumoto S, et al. Deletion of tetraspanin CD9 diminishes lymphangiogenesis in vivo and in vitro. J Biol Chem. 2013;288:2118-31 pubmed publisher
	Shaw C, Grobmyer S, Ucar D, Cance W, Reith J, Hochwald S. Elevated expression of IRS2 in the progression from neurofibroma to malignant peripheral nerve sheath tumor. Anticancer Res. 2012;32:439-43 pubmed
	Su C, Chen Z, Luo H, Su Y, Liu W, Cai L, et al. Different patterns of NF-?B and Notch1 signaling contribute to tumor-induced lymphangiogenesis of esophageal squamous cell carcinoma. J Exp Clin Cancer Res. 2011;30:85 pubmed publisher
	Moussai D, Mitsui H, Pettersen J, Pierson K, Shah K, Suárez Fariñas M, et al. The human cutaneous squamous cell carcinoma microenvironment is characterized by increased lymphatic density and enhanced expression of macrophage-derived VEGF-C. J Invest Dermatol. 2011;131:229-36 pubmed publisher
	Kubo M, Li T, Kamota T, Ohshima M, Shirasawa B, Hamano K. Extracorporeal shock wave therapy ameliorates secondary lymphedema by promoting lymphangiogenesis. J Vasc Surg. 2010;52:429-34 pubmed publisher
	Chen Z, Wang T, Luo H, Lai Y, Yang X, Li F, et al. Expression of nestin in lymph node metastasis and lymphangiogenesis in non-small cell lung cancer patients. Hum Pathol. 2010;41:737-44 pubmed publisher
	Henno A, Blacher S, Lambert C, Deroanne C, Noel A, Lapiere C, et al. Histological and transcriptional study of angiogenesis and lymphangiogenesis in uninvolved skin, acute pinpoint lesions and established psoriasis plaques: an approach of vascular development chronology in psoriasis. J Dermatol Sci. 2010;57:162-9 pubmed publisher

product information

Catalog Number :

MAB3757

Subcategory :

Cell Signaling

Product Name :

Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P15692

Host Name :

Mouse

Antigen :

VEGF Receptor-3

Clone :

9D9F9

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9

Cross Reactivity :

Human

Background :

Vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. {Bando, 2004}. Jussila et al reported that VEGFR3 antibodies specifically stain endothelial cells of lymphatic vessels and vessels around tumonrs such as lymphoma and in situ breast carcinoma. {Jussila, 1998}.Interestingly, the spindle cells of several cutaneous nodular AIDS-associated Kaposi's sarcomas and the endothelium around the nodules were also VEGFR-3 positive. The first specific molecular marker for the lymphatic endothelium should provide a useful tool for the analysis of lymphatic vessels in malignant tumors and their metastases and the cellular origin and differentiation of Kaposi's sarcomas. {Jussila, 1998}.

ALT Names :

VEGFR3; VEGFR-3; Receptor tyrosine Kinase VEGFR-3

Immunogen :

Recombinantly produced, purified, hVEGFR-3 extracellular domain protein from baculovirus culture {Jussila, 1998}.

Specificity :

Specific for human VEGFR-3. Western blot and ELISA demonstrate that the antibody is highly specific for VEGFR-3 with no cross-reactivity with human VEGFR-1 or VEGFR-2 protein {Bando, 2004}. Cross reactivity with other species VEGFR-3 proteins untested.

Package Size :

100 μL

Uses :

Flow Cytometry;Immunofluorescence;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.