LEF-1 Antibody, transactivation domain, clone 3A12 | EMD Millipore MAB3749 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

LEF-1 Antibody, transactivation domain, clone 3A12

catalog :

MAB3749

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A12

The same clone is also sold as:

Invitrogen: MA1-12486
OriGene: AM05274PU-N

reactivity :

human

application :

western blot

citations: 2

Reference
Cappato S, Tonachini L, Giacopelli F, Tirone M, Galietta L, Sormani M, et al. High-throughput screening for modulators of ACVR1 transcription: discovery of potential therapeutics for fibrodysplasia ossificans progressiva. Dis Model Mech. 2016;9:685-96 pubmed publisher
Fu X, Toh W, Liu H, Lu K, Li M, Cao T. Establishment of clinically compliant human embryonic stem cells in an autologous feeder-free system. Tissue Eng Part C Methods. 2011;17:927-37 pubmed publisher

product information

Catalog Number :

MAB3749

Subcategory :

Stem Cell Research

Product Name :

Anti-LEF-1 Antibody, transactivation domain, clone 3A12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UJU2

Host Name :

Mouse

Antigen :

LEF-1

Clone :

3A12

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-LEF-1 Antibody, transactivation domain, clone 3A12

Cross Reactivity :

Human

Background :

Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), andorthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.

ALT Names :

Lymphoid Enhancer Factor 1

Immunogen :

Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to amino acids 236-242 of human LEF-1 and mouse myeloma cells.

Specificity :

Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein.

Package Size :

100 µg

Uses :

Western Blotting

Storage :

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.