This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
catalog :
MAB367
quantity :
100 µg
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
M2-2-B3

The same clone is also sold as:
reactivity :
tiger salamander, human, mouse, rat, rhesus macaque
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 37
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; rat; 1:250; loading ...; fig 1a
  • immunohistochemistry; mouse; 1:250; loading ...; fig 4c
Katona L, Hartwich K, Tomioka R, Somogyi J, Roberts J, Wagner K, et al. Synaptic organisation and behaviour-dependent activity of mGluR8a-innervated GABAergic trilaminar cells projecting from the hippocampus to the subiculum. Brain Struct Funct. 2020;225:705-734 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 5c
Hamamoto M, Kiyokage E, Sohn J, Hioki H, Harada T, Toida K. Structural basis for cholinergic regulation of neural circuits in the mouse olfactory bulb. J Comp Neurol. 2017;525:574-591 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:750; loading ...; fig 2
Olsen G, Witter M. Posterior parietal cortex of the rat: Architectural delineation and thalamic differentiation. J Comp Neurol. 2016;524:3774-3809 pubmed publisher
  • immunohistochemistry; mouse; 1:500; fig 1
Lasiene J, Komine O, Fujimori Tonou N, Powers B, Endo F, Watanabe S, et al. Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons. Acta Neuropathol Commun. 2016;4:15 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Yang W, Carrasquillo Y, Hooks B, Nerbonne J, Burkhalter A. Distinct balance of excitation and inhibition in an interareal feedforward and feedback circuit of mouse visual cortex. J Neurosci. 2013;33:17373-84 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Csaba Z, Krejci E, Bernard V. Postsynaptic muscarinic m2 receptors at cholinergic and glutamatergic synapses of mouse brainstem motoneurons. J Comp Neurol. 2013;521:2008-24 pubmed publisher
  • immunohistochemistry; tiger salamander; 1:100
Cimini B, Strang C, Wotring V, Keyser K, Eldred W. Role of acetylcholine in nitric oxide production in the salamander retina. J Comp Neurol. 2008;507:1952-63 pubmed publisher
  • immunohistochemistry - free floating section; rhesus macaque; 1:2000
Saleem K, Price J, Hashikawa T. Cytoarchitectonic and chemoarchitectonic subdivisions of the perirhinal and parahippocampal cortices in macaque monkeys. J Comp Neurol. 2007;500:973-1006 pubmed
  • immunohistochemistry - frozen section; rat; 1:100
Jongen J, Jaarsma D, Hossaini M, Natarajan D, Haasdijk E, Holstege J. Distribution of RET immunoreactivity in the rodent spinal cord and changes after nerve injury. J Comp Neurol. 2007;500:1136-53 pubmed
D Souza R, Bista P, Meier A, Ji W, Burkhalter A. Spatial Clustering of Inhibition in Mouse Primary Visual Cortex. Neuron. 2019;104:588-600.e5 pubmed publisher
Salib M, Joshi A, Katona L, Howarth M, Micklem B, Somogyi P, et al. GABAergic Medial Septal Neurons with Low-Rhythmic Firing Innervating the Dentate Gyrus and Hippocampal Area CA3. J Neurosci. 2019;39:4527-4549 pubmed publisher
Lambert L, Dubayle D, Fafouri A, Herzog E, Csaba Z, Dournaud P, et al. Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway. Front Cell Neurosci. 2018;12:450 pubmed publisher
Gămănuţ R, Kennedy H, Toroczkai Z, Ercsey Ravasz M, Van Essen D, Knoblauch K, et al. The Mouse Cortical Connectome, Characterized by an Ultra-Dense Cortical Graph, Maintains Specificity by Distinct Connectivity Profiles. Neuron. 2018;97:698-715.e10 pubmed publisher
Fletcher M, Ogg M, Lu L, Ogg R, Boughter J. Overlapping Representation of Primary Tastes in a Defined Region of the Gustatory Cortex. J Neurosci. 2017;37:7595-7605 pubmed publisher
Fajardo Serrano A, Liu L, Mott D, McDonald A. Evidence for M2 muscarinic receptor modulation of axon terminals and dendrites in the rodent basolateral amygdala: An ultrastructural and electrophysiological analysis. Neuroscience. 2017;357:349-362 pubmed publisher
Milan L, Courtand G, Cardoit L, Masmejean F, Barrière G, Cazalets J, et al. Age-Related Changes in Pre- and Postsynaptic Partners of the Cholinergic C-Boutons in Wild-Type and SOD1G93A Lumbar Motoneurons. PLoS ONE. 2015;10:e0135525 pubmed publisher
Ortega Martinez S. Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress. Redox Biol. 2015;5:388-397 pubmed publisher
Smith M, Souza F, Bruce K, Strang C, Morley B, Keyser K. Acetylcholine receptors in the retinas of the α7 nicotinic acetylcholine receptor knockout mouse. Mol Vis. 2014;20:1328-56 pubmed
Traini C, Faussone Pellegrini M, Evangelista S, Mazzaferro K, Cipriani G, Santicioli P, et al. Inner and outer portions of colonic circular muscle: ultrastructural and immunohistochemical changes in rat chronically treated with otilonium bromide. PLoS ONE. 2014;9:e103237 pubmed publisher
Wang Q, Sporns O, Burkhalter A. Network analysis of corticocortical connections reveals ventral and dorsal processing streams in mouse visual cortex. J Neurosci. 2012;32:4386-99 pubmed publisher
Mavlyutov T, Epstein M, Liu P, Verbny Y, Ziskind Conhaim L, Ruoho A. Development of the sigma-1 receptor in C-terminals of motoneurons and colocalization with the N,N'-dimethyltryptamine forming enzyme, indole-N-methyl transferase. Neuroscience. 2012;206:60-8 pubmed publisher
McDonald A, Mascagni F. Neuronal localization of M2 muscarinic receptor immunoreactivity in the rat amygdala. Neuroscience. 2011;196:49-65 pubmed publisher
Kelada S, Wilson M, Tavarez U, Kubalanza K, Borate B, Whitehead G, et al. Strain-dependent genomic factors affect allergen-induced airway hyperresponsiveness in mice. Am J Respir Cell Mol Biol. 2011;45:817-24 pubmed publisher
Wang Q, Gao E, Burkhalter A. Gateways of ventral and dorsal streams in mouse visual cortex. J Neurosci. 2011;31:1905-18 pubmed publisher
Maison S, Liu X, Vetter D, Eatock R, Nathanson N, Wess J, et al. Muscarinic signaling in the cochlea: presynaptic and postsynaptic effects on efferent feedback and afferent excitability. J Neurosci. 2010;30:6751-62 pubmed publisher
Mavlyutov T, Epstein M, Andersen K, Ziskind Conhaim L, Ruoho A. The sigma-1 receptor is enriched in postsynaptic sites of C-terminals in mouse motoneurons. An anatomical and behavioral study. Neuroscience. 2010;167:247-55 pubmed publisher
Harrington A, Peck C, Liu L, Burcher E, Hutson J, Southwell B. Localization of muscarinic receptors M1R, M2R and M3R in the human colon. Neurogastroenterol Motil. 2010;22:999-1008, e262-3 pubmed publisher
Dobbertin A, Hrabovska A, Dembele K, Camp S, Taylor P, Krejci E, et al. Targeting of acetylcholinesterase in neurons in vivo: a dual processing function for the proline-rich membrane anchor subunit and the attachment domain on the catalytic subunit. J Neurosci. 2009;29:4519-30 pubmed publisher
Jositsch G, Papadakis T, Haberberger R, Wolff M, Wess J, Kummer W. Suitability of muscarinic acetylcholine receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice. Naunyn Schmiedebergs Arch Pharmacol. 2009;379:389-95 pubmed publisher
Harrington A, Hutson J, Southwell B. Immunohistochemical localisation of pre-synaptic muscarinic receptor subtype-2 (M2r) in the enteric nervous system of guinea-pig ileum. Cell Tissue Res. 2008;332:37-48 pubmed publisher
Oda S, Sato F, Okada A, Akahane S, Igarashi H, Yokofujita J, et al. Immunolocalization of muscarinic receptor subtypes in the reticular thalamic nucleus of rats. Brain Res Bull. 2007;74:376-84 pubmed
Salgado H, Bellay T, Nichols J, Bose M, Martinolich L, Perrotti L, et al. Muscarinic M2 and M1 receptors reduce GABA release by Ca2+ channel modulation through activation of PI3K/Ca2+ -independent and PLC/Ca2+ -dependent PKC. J Neurophysiol. 2007;98:952-65 pubmed
Takeuchi T, Tanaka K, Nakajima H, Matsui M, Azuma Y. M2 and M3 muscarinic receptors are involved in enteric nerve-mediated contraction of the mouse ileum: Findings obtained with muscarinic-receptor knockout mouse. Am J Physiol Gastrointest Liver Physiol. 2007;292:G154-64 pubmed
Takeuchi T, Toyoshima M, Mukai K, Hagi K, Matsui M, Nakajima H, et al. Involvement of M(2) muscarinic receptors in relaxant response of circular muscle of mouse gastric antrum. Neurogastroenterol Motil. 2006;18:226-33 pubmed
Iino S, Nojyo Y. Muscarinic M(2) acetylcholine receptor distribution in the guinea-pig gastrointestinal tract. Neuroscience. 2006;138:549-59 pubmed
Zucker C, Nilson J, Ehinger B, Grzywacz N. Compartmental localization of gamma-aminobutyric acid type B receptors in the cholinergic circuitry of the rabbit retina. J Comp Neurol. 2005;493:448-59 pubmed
Chaudhuri J, Hiltunen M, Nykanen M, Yla Herttuala S, Soininen H, Miettinen R. Localization of M2 muscarinic receptor protein in parvalbumin and calretinin containing cells of the adult rat entorhinal cortex using two complementary methods. Neuroscience. 2005;131:557-66 pubmed
product information
Catalog Number :
MAB367
Subcategory :
Neuroscience
Product Name :
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P08172
Host Name :
Rat
Antigen :
Muscarinic Acetylcholine Receptor m2
Clone :
M2-2-B3
Conjugate :
Purified
Isotype :
IgG2a
Product Description :
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
Cross Reactivity :
Human;Mammals;Monkey;Rat
Immunogen :
i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.
Specificity :
m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes. SPECIES REACTIVITIES: Expected to react with most mammalian species.
Package Size :
100 µg
Uses :
Immunocytochemistry;Immunohistochemistry;Immunoprecipitation
Storage :
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
  • • Cancer
  • • Nuclear Signaling
  • • Apoptosis
  • • Cardiovascular Disease
  • • Developmental Biology
  • • Metabolic Disease
  • • Neurodegenerative Disease
  • • Cell Biology
View all the new antibodies at www.emdmillipore.com.