BubR1 Antibody, clone 8G1 | EMD Millipore MAB3612 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BubR1 Antibody, clone 8G1

catalog :

MAB3612

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8G1

The same clone is also sold as:

Abnova: MAB1929
Invitrogen: MA5-16036, MA5-16038, MA5-16039, MA5-16037, MA1-16577, MA5-16035
Active Motif: 39747
LifeSpan Biosciences: LS-C141114, LS-C141115, LS-C141113, LS-C2771, LS-C18942, LS-C179698, LS-C141117
Novus Biologicals: NBP2-80606, NB100-353
Rockland Immunochemicals: 200-301-902
OriGene: TA301741
Santa Cruz Biotechnology: sc-47744

reactivity :

human, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:1000; loading ...; fig 3a	Vallardi G, Allan L, Crozier L, Saurin A. Division of labour between PP2A-B56 isoforms at the centromere and kinetochore. elife. 2019;8: pubmed publisher
immunocytochemistry; human; loading ...; fig 5c immunocytochemistry; Xenopus laevis; loading ...; fig 2b	Wynne D, Funabiki H. Heterogeneous architecture of vertebrate kinetochores revealed by three-dimensional superresolution fluorescence microscopy. Mol Biol Cell. 2016;27:3395-3404 pubmed
immunocytochemistry; human; 1:200; fig 1	Tauchman E, Boehm F, DeLuca J. Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Commun. 2015;6:10036 pubmed publisher
immunocytochemistry; human; 1:1000; tbl 3	Wynne D, Funabiki H. Kinetochore function is controlled by a phospho-dependent coexpansion of inner and outer components. J Cell Biol. 2015;210:899-916 pubmed
western blot; human	Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
immunoprecipitation; human; 1:500	Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher
immunoprecipitation; human; loading ...; fig 3g immunocytochemistry; human; loading ...; fig 3f western blot; human; loading ...; fig 4c	Espert A, Uluocak P, Bastos R, Mangat D, Graab P, Gruneberg U. PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing. J Cell Biol. 2014;206:833-42 pubmed publisher
western blot; human; fig 6c	Meyer H, Rape M. Enhanced protein degradation by branched ubiquitin chains. Cell. 2014;157:910-21 pubmed publisher
	Foley E, Maldonado M, Kapoor T. Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase. Nat Cell Biol. 2011;13:1265-71 pubmed publisher

product information

Catalog Number :

MAB3612

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BubR1 Antibody, clone 8G1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O60566

Host Name :

Mouse

Antigen :

BubR1

Clone :

8G1

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-BubR1 Antibody, clone 8G1

Cross Reactivity :

Human

Background :

The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. BubR1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.

Immunogen :

Recombinant human BubR1.

Specificity :

Recognizes human BubR1.

Package Size :

100 µg

Uses :

Western Blotting;Immunocytochemistry

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.