Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 | EMD Millipore MAB3584 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Heterochromatin Protein-1 α Antibody, clone 2HP-1H5

catalog :

MAB3584

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2HP-1H5

reactivity :

human, mouse, chicken

application :

immunohistochemistry, immunocytochemistry

citations: 26

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; loading ...; fig 1a	Ruppert J, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash A, et al. HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry. EMBO J. 2018;37: pubmed publisher
immunocytochemistry; chicken; 1:200; loading ...; fig 3b immunocytochemistry; human; 1:200; loading ...; fig 3a	de Castro I, Budzak J, Di Giacinto M, Ligammari L, Gokhan E, Spanos C, et al. Repo-Man/PP1 regulates heterochromatin formation in interphase. Nat Commun. 2017;8:14048 pubmed publisher
immunocytochemistry; mouse; fig 7	Brideau N, Coker H, Gendrel A, Siebert C, Bezstarosti K, Demmers J, et al. Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome. Mol Cell Biol. 2015;35:4053-68 pubmed publisher
immunohistochemistry; mouse	Akiyama T, Xin L, Oda M, Sharov A, Amano M, Piao Y, et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res. 2015;22:307-18 pubmed publisher
immunocytochemistry; mouse; 1:500; fig s3	Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-1470 pubmed publisher
immunocytochemistry; human	Culver Cochran A, Chadwick B. The WSTF-ISWI chromatin remodeling complex transiently associates with the human inactive X chromosome during late S-phase prior to BRCA1 and Î³-H2AX. PLoS ONE. 2012;7:e50023 pubmed publisher
	Yi Q, Chen Q, Yan H, Zhang M, Liang C, Xiang X, et al. Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion. J Biol Chem. 2019;294:2021-2035 pubmed publisher
	Djeghloul D, Kuranda K, Kuzniak I, Barbieri D, Naguibneva I, Choisy C, et al. Age-Associated Decrease of the Histone Methyltransferase SUV39H1 in HSC Perturbs Heterochromatin and B Lymphoid Differentiation. Stem Cell Reports. 2016;6:970-984 pubmed publisher
	Arai Y, Hayakawa K, Arai D, Ito R, Iwasaki Y, Saito K, et al. Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells. Biomed Res Int. 2015;2015:876047 pubmed publisher
	Wei X, Chen Y, Xu Y, Zhan Y, Zhang R, Wang M, et al. Small molecule compound induces chromatin de-condensation and facilitates induced pluripotent stem cell generation. J Mol Cell Biol. 2014;6:409-20 pubmed publisher
	Chakraborty A, Prasanth K, Prasanth S. Dynamic phosphorylation of HP1? regulates mitotic progression in human cells. Nat Commun. 2014;5:3445 pubmed publisher
	Nitta H, Unoki M, Ichiyanagi K, Kosho T, Shigemura T, Takahashi H, et al. Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J Hum Genet. 2013;58:455-60 pubmed publisher
	Choi J, Park M, Lee J. Suppression and recovery of BRCA1-mediated transcription by HP1? via modulation of promoter occupancy. Nucleic Acids Res. 2012;40:11321-38 pubmed publisher
	Ip J, Canham P, Choo K, Inaba Y, Jacobs S, Kalitsis P, et al. Normal DNA methylation dynamics in DICER1-deficient mouse embryonic stem cells. PLoS Genet. 2012;8:e1002919 pubmed publisher
	Chan K, Zhang Z. Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing. J Biol Chem. 2012;287:15024-33 pubmed publisher
	Shimura M, Toyoda Y, Iijima K, Kinomoto M, Tokunaga K, Yoda K, et al. Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation. J Cell Biol. 2011;194:721-35 pubmed publisher
	Inoue A, Hyle J, Lechner M, Lahti J. Mammalian ChlR1 has a role in heterochromatin organization. Exp Cell Res. 2011;317:2522-35 pubmed publisher
	Sugimura K, Fukushima Y, Ishida M, Ito S, Nakamura M, Mori Y, et al. Cell cycle-dependent accumulation of histone H3.3 and euchromatic histone modifications in pericentromeric heterochromatin in response to a decrease in DNA methylation levels. Exp Cell Res. 2010;316:2731-46 pubmed publisher
	Helbling Chadwick L, Chadwick B, Jaye D, Wade P. The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells. Chromosoma. 2009;118:445-57 pubmed publisher
	Frescas D, Guardavaccaro D, Kuchay S, Kato H, Poleshko A, Basrur V, et al. KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state. Cell Cycle. 2008;7:3539-47 pubmed
	Zwerger M, Herrmann H, Gaines P, Olins A, Olins D. Granulocytic nuclear differentiation of lamin B receptor-deficient mouse EPRO cells. Exp Hematol. 2008;36:977-87 pubmed publisher
	de la Cruz C, Kirmizis A, Simon M, Isono K, Koseki H, Panning B. The polycomb group protein SUZ12 regulates histone H3 lysine 9 methylation and HP1 alpha distribution. Chromosome Res. 2007;15:299-314 pubmed
	Kobayakawa S, Miike K, Nakao M, Abe K. Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells. 2007;12:447-60 pubmed
	Olins D, Olins A. Granulocyte heterochromatin: defining the epigenome. BMC Cell Biol. 2005;6:39 pubmed
	Spilianakis C, Lalioti M, Town T, Lee G, Flavell R. Interchromosomal associations between alternatively expressed loci. Nature. 2005;435:637-45 pubmed
	Guenatri M, Bailly D, Maison C, Almouzni G. Mouse centric and pericentric satellite repeats form distinct functional heterochromatin. J Cell Biol. 2004;166:493-505 pubmed

product information

Catalog Number :

MAB3584

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P45973

Host Name :

Mouse

Antigen :

Heterochromatin Protein-1 α

Clone :

2HP-1H5

Conjugate :

Ascites

Isotype :

IgG1κ

Product Description :

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5

Cross Reactivity :

Human;Mouse

Background :

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

ALT Names :

HP1a; Antigen p25; Chromobox Protein Homolog 5

Immunogen :

Recombinant mouse HP1a.

Specificity :

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

Package Size :

100 μL

Uses :

ELISA;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.