8-Oxoguanine Antibody, clone 483.15 | EMD Millipore MAB3560 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

8-Oxoguanine Antibody, clone 483.15

catalog :

MAB3560

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

483.15

reactivity :

human, mouse

application :

immunocytochemistry, flow cytometry, chromatin immunoprecipitation

citations: 22

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 5g	Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher
immunocytochemistry; human; 1:200; loading ...; fig 2	Tan R, Lan L. Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins. Methods Mol Biol. 2017;1587:139-146 pubmed publisher
immunocytochemistry; mouse; fig 2a	Iarriccio L, ManguÃ¡n GarcÃa C, Pintado Berninches L, MancheÃ±o J, Molina A, Perona R, et al. GSE4, a Small Dyskerin- and GSE24.2-Related Peptide, Induces Telomerase Activity, Cell Proliferation and Reduces DNA Damage, Oxidative Stress and Cell Senescence in Dyskerin Mutant Cells. PLoS ONE. 2015;10:e0142980 pubmed publisher
immunocytochemistry; human; fig 6	Yu Z, Huang Y, Shieh S. Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation. Nucleic Acids Res. 2016;44:1133-50 pubmed publisher
flow cytometry; human; 1:100	Lowe R, Overhoff M, Ramagopalan S, Garbe J, Koh J, Stampfer M, et al. The senescent methylome and its relationship with cancer, ageing and germline genetic variation in humans. Genome Biol. 2015;16:194 pubmed publisher
immunocytochemistry; human	Manguan García C, Pintado Berninches L, Carrillo J, Machado Pinilla R, Sastre L, Perez Quilis C, et al. Expression of the genetic suppressor element 24.2 (GSE24.2) decreases DNA damage and oxidative stress in X-linked dyskeratosis congenita cells. PLoS ONE. 2014;9:e101424 pubmed publisher
	Zhuo M, Gorgun M, Englander E. Neurotoxicity of cytarabine (Ara-C) in dorsal root ganglion neurons originates from impediment of mtDNA synthesis and compromise of mitochondrial function. Free Radic Biol Med. 2018;121:9-19 pubmed publisher
	Denver P, English A, McClean P. Inflammation, insulin signaling and cognitive function in aged APP/PS1 mice. Brain Behav Immun. 2018;70:423-434 pubmed publisher
	Ritfeld G, Rauck B, Novosat T, Park D, Patel P, Roos R, et al. The effect of a polyurethane-based reverse thermal gel on bone marrow stromal cell transplant survival and spinal cord repair. Biomaterials. 2014;35:1924-31 pubmed publisher
	Parthsarathy V, McClean P, Holscher C, Taylor M, Tinker C, Jones G, et al. A novel retro-inverso peptide inhibitor reduces amyloid deposition, oxidation and inflammation and stimulates neurogenesis in the APPswe/PS1?E9 mouse model of Alzheimer's disease. PLoS ONE. 2013;8:e54769 pubmed publisher
	Jung S, Kim Y. C-terminal region of HBx is crucial for mitochondrial DNA damage. Cancer Lett. 2013;331:76-83 pubmed publisher
	Peng D, Belkhiri A, Hu T, Chaturvedi R, Asim M, Wilson K, et al. Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells. Gut. 2012;61:1250-60 pubmed publisher
	Zaika E, Wei J, Yin D, Andl C, Moll U, El Rifai W, et al. p73 protein regulates DNA damage repair. FASEB J. 2011;25:4406-14 pubmed publisher
	Mehmeti I, Gurgul Convey E, Lenzen S, Lortz S. Induction of the intrinsic apoptosis pathway in insulin-secreting cells is dependent on oxidative damage of mitochondria but independent of caspase-12 activation. Biochim Biophys Acta. 2011;1813:1827-35 pubmed publisher
	Lauritzen K, Cheng C, Wiksen H, Bergersen L, Klungland A. Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses. DNA Repair (Amst). 2011;10:639-53 pubmed publisher
	Yoshioka H, Niizuma K, Katsu M, Sakata H, Okami N, Chan P. Consistent injury to medium spiny neurons and white matter in the mouse striatum after prolonged transient global cerebral ischemia. J Neurotrauma. 2011;28:649-60 pubmed publisher
	Yoshioka H, Niizuma K, Katsu M, Okami N, Sakata H, Kim G, et al. NADPH oxidase mediates striatal neuronal injury after transient global cerebral ischemia. J Cereb Blood Flow Metab. 2011;31:868-80 pubmed publisher
	Sobinoff A, Pye V, Nixon B, Roman S, McLaughlin E. Adding insult to injury: effects of xenobiotic-induced preantral ovotoxicity on ovarian development and oocyte fusibility. Toxicol Sci. 2010;118:653-66 pubmed publisher
	Ba X, Gupta S, Davidson M, Garg N. Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes. J Biol Chem. 2010;285:11596-606 pubmed publisher
	Foo S, Heller E, Wykrzykowska J, Sullivan C, Manning Tobin J, Moore K, et al. Vascular effects of a low-carbohydrate high-protein diet. Proc Natl Acad Sci U S A. 2009;106:15418-23 pubmed publisher
	Polytarchou C, Pfau R, Hatziapostolou M, Tsichlis P. The JmjC domain histone demethylase Ndy1 regulates redox homeostasis and protects cells from oxidative stress. Mol Cell Biol. 2008;28:7451-64 pubmed publisher
	Sordet O, Goldman A, Redon C, Solier S, Rao V, Pommier Y. Topoisomerase I requirement for death receptor-induced apoptotic nuclear fission. J Biol Chem. 2008;283:23200-8 pubmed publisher

product information

Catalog Number :

MAB3560

Subcategory :

Neuroscience

Product Name :

Anti-8-Oxoguanine Antibody, clone 483.15

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

8-Oxoguanine

Clone :

483.15

Conjugate :

Ascites

Isotype :

IgM

Product Description :

Anti-8-Oxoguanine Antibody, clone 483.15

Cross Reactivity :

Human;Rat;Mouse;Monkey

Background :

8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosly linked to several cancers and diseases, such as Alzheimer's and Parkinson's.

ALT Names :

8-oxoG

Immunogen :

8-oxoguanine adsorbed on to alumina

Specificity :

8-oxoguanine. By competitive ELISA the monoclonal showed no reactivity with dGMP, dAMP, dCMP or TMP in micromolar concentrations. Competition was only observed when the concentrations were increased to the millimolar range.

Package Size :

100 µL

Uses :

ELISA;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance.