Lamin A Antibody, CT, a.a. 598-611, clone 133A2 | EMD Millipore MAB3540 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Lamin A Antibody, CT, a.a. 598-611, clone 133A2

catalog :

MAB3540

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

133A2

The same clone is also sold as:

Novus Biologicals: NBP1-97688
Abnova: MAB2470, MAB16237
LifeSpan Biosciences: LS-B7178
Invitrogen: MA1-06101
OriGene: BM4500
Santa Cruz Biotechnology: sc-56137
Abcam: ab8980

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 4b	Wang W, Wang J, Lin W, Kao C, Hung M, Teng Y, et al. Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis. Aging Cell. 2020;19:e13090 pubmed publisher
western blot; human; 1:5000; loading ...; fig 5b	Peng J, Liang S, Li L. sFRP1 exerts effects on gastric cancer cells through GSK3β/Rac1‑mediated restraint of TGFβ/Smad3 signaling. Oncol Rep. 2019;41:224-234 pubmed publisher
immunocytochemistry; human western blot; human	Pacheco L, Gomez L, Dias J, Ziebarth N, Howard G, Schiller P. Progerin expression disrupts critical adult stem cell functions involved in tissue repair. Aging (Albany NY). 2014;6:1049-63 pubmed
immunohistochemistry; mouse; 1:400	Jung H, Tatar A, Tu Y, Nobumori C, Yang S, Goulbourne C, et al. An absence of nuclear lamins in keratinocytes leads to ichthyosis, defective epidermal barrier function, and intrusion of nuclear membranes and endoplasmic reticulum into the nuclear chromatin. Mol Cell Biol. 2014;34:4534-44 pubmed publisher
western blot; rat; 1:500	Padiya R, Chowdhury D, Borkar R, Srinivas R, Pal Bhadra M, Banerjee S. Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat. PLoS ONE. 2014;9:e94228 pubmed publisher
western blot; rat; fig 2 western blot; mouse; fig 4a	Chua J, Reddy S, Merry D, Adachi H, Katsuno M, Sobue G, et al. Transcriptional activation of TFEB/ZKSCAN3 target genes underlies enhanced autophagy in spinobulbar muscular atrophy. Hum Mol Genet. 2014;23:1376-86 pubmed publisher
	Takamori Y, Hirahara Y, Wakabayashi T, Mori T, Koike T, Kataoka Y, et al. Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex. IBRO Rep. 2018;5:99-109 pubmed publisher
	Sehnalová P, Legartova S, Cmarko D, Kozubek S, Bartova E. Recruitment of HP1? to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins. Biol Cell. 2014;106:151-65 pubmed publisher
	Mahen R, Hattori H, Lee M, Sharma P, Jeyasekharan A, Venkitaraman A. A-type lamins maintain the positional stability of DNA damage repair foci in mammalian nuclei. PLoS ONE. 2013;8:e61893 pubmed publisher
	Wakabayashi T, Mori T, Hirahara Y, Koike T, Kubota Y, Takamori Y, et al. Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina. Histochem Cell Biol. 2011;136:427-36 pubmed publisher
	Drummond M, Fry C, Glynn E, Timmerman K, Dickinson J, Walker D, et al. Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise. J Appl Physiol (1985). 2011;111:135-42 pubmed publisher
	Coffinier C, Jung H, Li Z, Nobumori C, Yun U, Farber E, et al. Direct synthesis of lamin A, bypassing prelamin a processing, causes misshapen nuclei in fibroblasts but no detectable pathology in mice. J Biol Chem. 2010;285:20818-26 pubmed publisher
	Stradalova V, Gaplovska Kysela K, Hozak P. Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells. Histochem Cell Biol. 2008;130:1047-52 pubmed publisher

product information

Catalog Number :

MAB3540

Subcategory :

Cell Structure

Product Name :

Anti-Lamin A Antibody, CT, a.a. 598-611, clone 133A2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P02545

Host Name :

Mouse

Antigen :

Lamin A

Clone :

133A2

Conjugate :

Purified

Isotype :

IgG3

Product Description :

Anti-Lamin A Antibody, CT, a.a. 598-611, clone 133A2

Cross Reactivity :

Bovine;Canine;Human;Mouse;Rat

Background :

Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

ALT Names :

70 kDa Lamin

Immunogen :

Synthetic peptide from the C-terminus of human lamin A. This sequence is not present in the the lamin C isoform.

Specificity :

Monoclonal 133A2 reacts against human lamin A but not lamin C. The epitope lies with the 98 amino acids of the C terminus that is unique to lamin A. Specifically deletion analysis has shown that amino acids 598-611 were essential for reactivity {Hozak, P et.al. (1995), J. Cell Sci 635-644}.

Package Size :

100 µg

Uses :

Flow Cytometry;Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.