Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6 | EMD Millipore MAB351R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6

catalog :

MAB351R

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GAD-6

The same clone is also sold as:

BD Biosciences: 559931
Santa Cruz Biotechnology: sc-32270
Developmental Studies Hybridoma Bank: GAD-6
MilliporeSigma: G1166
Abcam: ab26113

reactivity :

Mongolian jird, human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; rat; 1:2,000	Noam Y, Phan L, McClelland S, Manders E, Ehrengruber M, Wadman W, et al. Distinct regional and subcellular localization of the actin-binding protein filamin A in the mature rat brain. J Comp Neurol. 2012;520:3013-34 pubmed publisher
immunohistochemistry - free floating section; Mongolian jird; 1:500	Kaiser A, Alexandrova O, Grothe B. Urocortin-expressing olivocochlear neurons exhibit tonotopic and developmental changes in the auditory brainstem and in the innervation of the cochlea. J Comp Neurol. 2011;519:2758-78 pubmed publisher
immunohistochemistry - free floating section; rat; 1:1,000	Rostkowski A, Teppen T, Peterson D, Urban J. Cell-specific expression of neuropeptide Y Y1 receptor immunoreactivity in the rat basolateral amygdala. J Comp Neurol. 2009;517:166-76 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:2,000 immunohistochemistry - frozen section; rat; 1:2,000	Landry M, Bouali Benazzouz R, Andre C, Shi T, Léger C, Nagy F, et al. Galanin receptor 1 is expressed in a subpopulation of glutamatergic interneurons in the dorsal horn of the rat spinal cord. J Comp Neurol. 2006;499:391-403 pubmed
	Favuzzi E, Marques Smith A, Deogracias R, Winterflood C, Sánchez Aguilera A, Mantoan L, et al. Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican. Neuron. 2017;95:639-655.e10 pubmed publisher
	Weltzien F, Percival K, Martin P, GrÃ¼nert U. Analysis of bipolar and amacrine populations in marmoset retina. J Comp Neurol. 2015;523:313-34 pubmed publisher
	Song C, Feodorova Y, Guy J, Peichl L, Jost K, Kimura H, et al. DNA methylation reader MECP2: cell type- and differentiation stage-specific protein distribution. Epigenetics Chromatin. 2014;7:17 pubmed publisher
	Soussi R, Boulland J, Bassot E, Bras H, Coulon P, Chaudhry F, et al. Reorganization of supramammillary-hippocampal pathways in the rat pilocarpine model of temporal lobe epilepsy: evidence for axon terminal sprouting. Brain Struct Funct. 2015;220:2449-68 pubmed publisher
	Gomez Varela D, Schmidt M, Schoellerman J, Peters E, Berg D. PMCA2 via PSD-95 controls calcium signaling by ?7-containing nicotinic acetylcholine receptors on aspiny interneurons. J Neurosci. 2012;32:6894-905 pubmed publisher
	Gribaudo S, Bovetti S, Friard O, Denorme M, Oboti L, Fasolo A, et al. Transitory and activity-dependent expression of neurogranin in olfactory bulb tufted cells during mouse postnatal development. J Comp Neurol. 2012;520:3055-69 pubmed publisher
	Boulland J, Chaudhry F. Ontogenetic changes in the distribution of the vesicular GABA transporter VGAT correlate with the excitation/inhibition shift of GABA action. Neurochem Int. 2012;61:506-16 pubmed publisher
	Gorelova N, Mulholland P, Chandler L, Seamans J. The glutamatergic component of the mesocortical pathway emanating from different subregions of the ventral midbrain. Cereb Cortex. 2012;22:327-36 pubmed publisher
	de Melo J, Peng G, Chen S, Blackshaw S. The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons. Development. 2011;138:2325-36 pubmed publisher
	Soussi R, Zhang N, Tahtakran S, Houser C, Esclapez M. Heterogeneity of the supramammillary-hippocampal pathways: evidence for a unique GABAergic neurotransmitter phenotype and regional differences. Eur J Neurosci. 2010;32:771-85 pubmed publisher
	Gallagher S, Witkovsky P, Roux M, Low M, Otero Corchon V, Hentges S, et al. beta-Endorphin expression in the mouse retina. J Comp Neurol. 2010;518:3130-48 pubmed publisher
	Guo C, Hirano A, Stella S, Bitzer M, Brecha N. Guinea pig horizontal cells express GABA, the GABA-synthesizing enzyme GAD 65, and the GABA vesicular transporter. J Comp Neurol. 2010;518:1647-69 pubmed publisher
	Commons K. Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3). J Chem Neuroanat. 2009;38:273-81 pubmed publisher
	Di Cristo G, Chattopadhyaya B, Kuhlman S, Fu Y, Bélanger M, Wu C, et al. Activity-dependent PSA expression regulates inhibitory maturation and onset of critical period plasticity. Nat Neurosci. 2007;10:1569-77 pubmed
	Sun C, Mtchedlishvili Z, Erisir A, Kapur J. Diminished neurosteroid sensitivity of synaptic inhibition and altered location of the alpha4 subunit of GABA(A) receptors in an animal model of epilepsy. J Neurosci. 2007;27:12641-50 pubmed
	Ankelo M, Westerlund A, Blomberg K, Knip M, Ilonen J, Hinkkanen A. Time-resolved immunofluorometric dual-label assay for simultaneous detection of autoantibodies to GAD65 and IA-2 in children with type 1 diabetes. Clin Chem. 2007;53:472-9 pubmed

product information

Catalog Number :

MAB351R

Subcategory :

Neuroscience

Product Name :

Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q05329

Host Name :

Mouse

Antigen :

Glutamate Decarboxylase

Clone :

GAD-6

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6

Cross Reactivity :

Human;Rat

Background :

Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kDa and 67kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585aa), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594aa.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk et al., 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.

ALT Names :

GAD65

Immunogen :

Purified rat brain GAD.

Specificity :

Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain (Gottlieb, et al., 1986; Chang & Gottlieb, 1988). This monclonal antibody can be used for immunohistochemical localization in brain or pancreas. Anti-GAD has also been used to label purified GAD on Western blots (Chang & Gottlieb, 1988).

Package Size :

100 μg

Uses :

Immunohistochemistry;Western Blotting

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.