BrdU Antibody, clone BU-1, Peroxidase conjugated | EMD Millipore MAB3510P product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BrdU Antibody, clone BU-1, Peroxidase conjugated

catalog :

MAB3510P

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

BU-1

application :

immunohistochemistry, immunocytochemistry, flow cytometry

citations: 4

Reference
Kang S, Keasey M, Hagg T. P2X7 receptor inhibition increases CNTF in the subventricular zone, but not neurogenesis or neuroprotection after stroke in adult mice. Transl Stroke Res. 2013;4:533-45 pubmed publisher
Kang S, Keasey M, Arnold S, Reid R, Geralds J, Hagg T. Endogenous CNTF mediates stroke-induced adult CNS neurogenesis in mice. Neurobiol Dis. 2013;49:68-78 pubmed publisher
Arnold S, Hagg T. Serotonin 1A receptor agonist increases species- and region-selective adult CNS proliferation, but not through CNTF. Neuropharmacology. 2012;63:1238-47 pubmed publisher
Mahoney E, Benton R, Maddie M, Whittemore S, Hagg T. ADAM8 is selectively up-regulated in endothelial cells and is associated with angiogenesis after spinal cord injury in adult mice. J Comp Neurol. 2009;512:243-55 pubmed publisher

product information

Catalog Number :

MAB3510P

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BrdU Antibody, clone BU-1, Peroxidase conjugated

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

BrdU

Clone :

BU-1

Conjugate :

HRP

Isotype :

IgG2a

Product Description :

Anti-BrdU Antibody, clone BU-1, Peroxidase conjugated

Cross Reactivity :

All

Background :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated.

ALT Names :

BrdU

Immunogen :

Bromodeoxyuridine

Specificity :

The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromodeoxyuridine does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine. MAB3510P does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU.

Package Size :

100 µg

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 6 months. Avoid freezing after reconsituition.