BrdU Antibody, clone BU-1, no acid or PK required | EMD Millipore MAB3510 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BrdU Antibody, clone BU-1, no acid or PK required

catalog :

MAB3510

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BU-1

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 27

Reference
Gong Y, Handa N, Kowalczykowski S, de Lange T. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev. 2017;31:46-58 pubmed publisher
Du R, Wu F, Lu M, Shu X, Ding J, Wu G, et al. Uncoupling protein 2 modulation of the NLRP3 inflammasome in astrocytes and its implications in depression. Redox Biol. 2016;9:178-187 pubmed publisher
Di Pietro C, Marazziti D, La Sala G, Abbaszadeh Z, Golini E, Matteoni R, et al. Primary Cilia in the Murine Cerebellum and in Mutant Models of Medulloblastoma. Cell Mol Neurobiol. 2017;37:145-154 pubmed publisher
Chen C, Kim K, Lau L. The matricellular protein CCN1 suppresses hepatocarcinogenesis by inhibiting compensatory proliferation. Oncogene. 2016;35:1314-23 pubmed publisher
Kang S, Keasey M, Hagg T. P2X7 receptor inhibition increases CNTF in the subventricular zone, but not neurogenesis or neuroprotection after stroke in adult mice. Transl Stroke Res. 2013;4:533-45 pubmed publisher
Xu Z, Gao Y, Xu F. Deficits of peripheral olfactory inputs reduce cell proliferation in the adult subventricular and subgranular zones. Neurosci Lett. 2013;541:269-74 pubmed publisher
Suetsugu Maki R, Maki N, Nakamura K, Sumanas S, Zhu J, Del Rio Tsonis K, et al. Lens regeneration in axolotl: new evidence of developmental plasticity. BMC Biol. 2012;10:103 pubmed publisher
Kang S, Keasey M, Arnold S, Reid R, Geralds J, Hagg T. Endogenous CNTF mediates stroke-induced adult CNS neurogenesis in mice. Neurobiol Dis. 2013;49:68-78 pubmed publisher
Arnold S, Hagg T. Serotonin 1A receptor agonist increases species- and region-selective adult CNS proliferation, but not through CNTF. Neuropharmacology. 2012;63:1238-47 pubmed publisher
Myohara M. What role do annelid neoblasts play? A comparison of the regeneration patterns in a neoblast-bearing and a neoblast-lacking enchytraeid oligochaete. PLoS ONE. 2012;7:e37319 pubmed publisher
Tian C, Gong Y, Yang Y, Shen W, Wang K, Liu J, et al. Foxg1 has an essential role in postnatal development of the dentate gyrus. J Neurosci. 2012;32:2931-49 pubmed publisher
Huang X, Kong H, Tang M, Lu M, Ding J, Hu G. D-Serine regulates proliferation and neuronal differentiation of neural stem cells from postnatal mouse forebrain. CNS Neurosci Ther. 2012;18:4-13 pubmed publisher
Suetsugu Maki R, Maki N, Fox T, Nakamura K, Cowper Solari R, Tomlinson C, et al. A complement receptor C5a antagonist regulates epithelial to mesenchymal transition and crystallin expression after lens cataract surgery in mice. Mol Vis. 2011;17:949-64 pubmed
Li Y, Tian C, Yang Y, Yan Y, Ni Y, Wei Y, et al. An inducible transgenic Cre mouse line for the study of hippocampal development and adult neurogenesis. Genesis. 2011;49:919-26 pubmed publisher
Tsonis P, Haynes T, Maki N, Nakamura K, Casco Robles M, Yamada S, et al. Controlling gene loss of function in newts with emphasis on lens regeneration. Nat Protoc. 2011;6:593-9 pubmed publisher
Dizon M, Maa T, Kessler J. The bone morphogenetic protein antagonist noggin protects white matter after perinatal hypoxia-ischemia. Neurobiol Dis. 2011;42:318-26 pubmed publisher
Maki N, Tsonis P, Agata K. Changes in global histone modifications during dedifferentiation in newt lens regeneration. Mol Vis. 2010;16:1893-7 pubmed
Nakamura K, Maki N, Trinh A, Trask H, Gui J, Tomlinson C, et al. miRNAs in newt lens regeneration: specific control of proliferation and evidence for miRNA networking. PLoS ONE. 2010;5:e12058 pubmed publisher
Dizon M, SZELE F, Kessler J. Hypoxia-ischemia induces an endogenous reparative response by local neural progenitors in the postnatal mouse telencephalon. Dev Neurosci. 2010;32:173-83 pubmed publisher
Maki N, Suetsugu Maki R, Sano S, Nakamura K, Nishimura O, Tarui H, et al. Oocyte-type linker histone B4 is required for transdifferentiation of somatic cells in vivo. FASEB J. 2010;24:3462-7 pubmed publisher
Sun X, Jiang R, Zhang Y, Chen M, Xiang P, Qi Y, et al. Gene expression and differentiation characteristics in mice E13.5 and E17.5 neural retinal progenitors. Mol Vis. 2009;15:2503-14 pubmed
Merlo S, Frasca G, Canonico P, Sortino M. Differential involvement of estrogen receptor alpha and estrogen receptor beta in the healing promoting effect of estrogen in human keratinocytes. J Endocrinol. 2009;200:189-97 pubmed publisher
Mahoney E, Benton R, Maddie M, Whittemore S, Hagg T. ADAM8 is selectively up-regulated in endothelial cells and is associated with angiogenesis after spinal cord injury in adult mice. J Comp Neurol. 2009;512:243-55 pubmed publisher
Chang M, Wu C, Jiang Shieh Y, Shieh J, Wen C. Reactive changes of retinal astrocytes and Müller glial cells in kainate-induced neuroexcitotoxicity. J Anat. 2007;210:54-65 pubmed
Yang P, Baker K, Hagg T. A disintegrin and metalloprotease 21 (ADAM21) is associated with neurogenesis and axonal growth in developing and adult rodent CNS. J Comp Neurol. 2005;490:163-79 pubmed
Baker S, Baker K, Hagg T. D3 dopamine receptors do not regulate neurogenesis in the subventricular zone of adult mice. Neurobiol Dis. 2005;18:523-7 pubmed
Rafuse V, Soundararajan P, Leopold C, Robertson H. Neuroprotective properties of cultured neural progenitor cells are associated with the production of sonic hedgehog. Neuroscience. 2005;131:899-916 pubmed

product information

Catalog Number :

MAB3510

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BrdU Antibody, clone BU-1, no acid or PK required

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

BrdU

Clone :

BU-1

Conjugate :

Culture Supernatant

Isotype :

IgG2a

Product Description :

Anti-BrdU Antibody, clone BU-1, no acid or PK required

Cross Reactivity :

All

Background :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously.

ALT Names :

BrdU

Immunogen :

Bromodeoxyuridine

Specificity :

Specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromodeoxyuridine does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine. MAB3510 does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.

Package Size :

100 μL

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.