This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
catalog :
MAB3448
quantity :
100 μL
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1MOD-1A9
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 53
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 5a
Bartova E, Lochmanová G, Legartova S, Suchankova J, Fedr R, Krejci J, et al. Irradiation by γ-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and γH2AX. Biochimie. 2018;154:86-98 pubmed publisher
  • western blot; human; loading ...; fig 1c
Wang Y, Wang Y, Ma L, Nie M, Ju J, Liu M, et al. Heterochromatin Protein 1γ Is a Novel Epigenetic Repressor of Human Embryonic ϵ-Globin Gene Expression. J Biol Chem. 2017;292:4811-4817 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s5b
Hansen R, Mund A, Poulsen S, Sandoval M, Klement K, Tsouroula K, et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol. 2016;18:1357-1366 pubmed publisher
  • western blot; human; 1:10,000; fig 1
Hiragami Hamada K, Soeroes S, Nikolov M, Wilkins B, Kreuz S, Chen C, et al. Dynamic and flexible H3K9me3 bridging via HP1? dimerization establishes a plastic state of condensed chromatin. Nat Commun. 2016;7:11310 pubmed publisher
  • western blot; human; fig 4a
Baude A, Aaes T, Zhai B, Al Nakouzi N, Oo H, Daugaard M, et al. Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. Nucleic Acids Res. 2016;44:2214-26 pubmed publisher
  • western blot; human
Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami Hamada K, Nakagawa R, et al. N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity. Nucleic Acids Res. 2014;42:12498-511 pubmed publisher
  • western blot; mouse
Popova E, Grigoryev S, Fan Y, Skoultchi A, Zhang S, Barnstable C. Developmentally regulated linker histone H1c promotes heterochromatin condensation and mediates structural integrity of rod photoreceptors in mouse retina. J Biol Chem. 2013;288:17895-907 pubmed publisher
  • immunocytochemistry; human
Culver Cochran A, Chadwick B. The WSTF-ISWI chromatin remodeling complex transiently associates with the human inactive X chromosome during late S-phase prior to BRCA1 and γ-H2AX. PLoS ONE. 2012;7:e50023 pubmed publisher
  • immunocytochemistry; human
Mao Z, Hine C, Tian X, Van Meter M, Au M, Vaidya A, et al. SIRT6 promotes DNA repair under stress by activating PARP1. Science. 2011;332:1443-6 pubmed publisher
Legartova S, Lochmanová G, Zdrahal Z, Kozubek S, Sponer J, Krepl M, et al. DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88. Cells. 2019;8: pubmed publisher
Yi Q, Chen Q, Yan H, Zhang M, Liang C, Xiang X, et al. Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion. J Biol Chem. 2019;294:2021-2035 pubmed publisher
Svobodová Kovaříková A, Legartova S, Krejci J, Bartova E. H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions. Aging (Albany NY). 2018;10:2585-2605 pubmed publisher
Bartova E, Legartova S, Krejci J, Řezníčková P, Kovaříková A, Suchankova J, et al. Depletion of A-type lamins and Lap2? reduces 53BP1 accumulation at UV-induced DNA lesions and Lap2? protein is responsible for compactness of irradiated chromatin. J Cell Biochem. 2018;: pubmed publisher
Lin C, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, et al. A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed. Sci Rep. 2018;8:2743 pubmed publisher
Müllers E, Silva Cascales H, Burdova K, Macurek L, Lindqvist A. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell. 2017;16:575-584 pubmed publisher
Herberg S, Simeone A, Oikawa M, Jullien J, Bradshaw C, Teperek M, et al. Histone H3 lysine 9 trimethylation is required for suppressing the expression of an embryonically activated retrotransposon in Xenopus laevis. Sci Rep. 2015;5:14236 pubmed publisher
Stixová L, Sehnalová P, Legartová S, Suchánková J, Hrušková T, Kozubek S, et al. HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs. Epigenetics Chromatin. 2014;7:39 pubmed publisher
Irvine K, Skoien R, Bokil N, Melino M, Thomas G, Loo D, et al. Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration. World J Gastroenterol. 2014;20:17851-62 pubmed publisher
Van Meter M, Kashyap M, Rezazadeh S, Geneva A, Morello T, Seluanov A, et al. SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and age. Nat Commun. 2014;5:5011 pubmed publisher
Chakraborty A, Prasanth K, Prasanth S. Dynamic phosphorylation of HP1? regulates mitotic progression in human cells. Nat Commun. 2014;5:3445 pubmed publisher
Takebayashi S, Lei I, Ryba T, Sasaki T, Dileep V, Battaglia D, et al. Murine esBAF chromatin remodeling complex subunits BAF250a and Brg1 are necessary to maintain and reprogram pluripotency-specific replication timing of select replication domains. Epigenetics Chromatin. 2013;6:42 pubmed publisher
Dowdle J, Mehta M, Kass E, Vuong B, Inagaki A, Egli D, et al. Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis. PLoS Genet. 2013;9:e1003945 pubmed publisher
Cronin J, Watkins Chow D, Incao A, Hasskamp J, Schönewolf N, Aoude L, et al. SOX10 ablation arrests cell cycle, induces senescence, and suppresses melanomagenesis. Cancer Res. 2013;73:5709-18 pubmed publisher
Sdek P, Oyama K, Angelis E, Chan S, Schenke Layland K, MacLellan W. Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha. PLoS ONE. 2013;8:e58319 pubmed publisher
Aird K, Zhang R. Detection of senescence-associated heterochromatin foci (SAHF). Methods Mol Biol. 2013;965:185-96 pubmed publisher
Choi J, Park M, Lee J. Suppression and recovery of BRCA1-mediated transcription by HP1? via modulation of promoter occupancy. Nucleic Acids Res. 2012;40:11321-38 pubmed publisher
Legartova S, Jugova A, Stixova L, Kozubek S, Fojtova M, Zdrahal Z, et al. Epigenetic aspects of HP1 exchange kinetics in apoptotic chromatin. Biochimie. 2013;95:167-79 pubmed publisher
Liu Y, Nakamura T, Nakano T. Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis. Biol Reprod. 2012;86:40 pubmed publisher
Hamilton C, Hayward R, Gilbert N. Global chromatin fibre compaction in response to DNA damage. Biochem Biophys Res Commun. 2011;414:820-5 pubmed publisher
Inoue A, Hyle J, Lechner M, Lahti J. Mammalian ChlR1 has a role in heterochromatin organization. Exp Cell Res. 2011;317:2522-35 pubmed publisher
Weyemi U, Lagente Chevallier O, Boufraqech M, Prenois F, Courtin F, Caillou B, et al. ROS-generating NADPH oxidase NOX4 is a critical mediator in oncogenic H-Ras-induced DNA damage and subsequent senescence. Oncogene. 2012;31:1117-29 pubmed publisher
Sdek P, Zhao P, Wang Y, Huang C, Ko C, Butler P, et al. Rb and p130 control cell cycle gene silencing to maintain the postmitotic phenotype in cardiac myocytes. J Cell Biol. 2011;194:407-23 pubmed publisher
Sustáčková G, Kozubek S, Stixova L, Legartova S, Matula P, Orlova D, et al. Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin. J Cell Physiol. 2012;227:1838-50 pubmed publisher
Xu Y, Cui X, Tae J, Jin Y, Kim N. DNA synthesis and epigenetic modification during mouse oocyte fertilization by human or hamster sperm injection. J Assist Reprod Genet. 2011;28:325-33 pubmed publisher
Higo S, Asano Y, Kato H, Yamazaki S, Nakano A, Tsukamoto O, et al. Isoform-specific intermolecular disulfide bond formation of heterochromatin protein 1 (HP1). J Biol Chem. 2010;285:31337-47 pubmed publisher
Maehara K, Takahashi K, Saitoh S. CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses. Mol Cell Biol. 2010;30:2090-104 pubmed publisher
Horáková A, Bartova E, Galiova G, Uhlírová R, Matula P, Kozubek S. SUV39h-independent association of HP1 beta with fibrillarin-positive nucleolar regions. Chromosoma. 2010;119:227-41 pubmed publisher
Wu S, Hu Y, Liu H, Shi Y. Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis. Mol Cell Biol. 2009;29:6245-56 pubmed publisher
Helbling Chadwick L, Chadwick B, Jaye D, Wade P. The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells. Chromosoma. 2009;118:445-57 pubmed publisher
Takanashi M, Oikawa K, Fujita K, Kudo M, Kinoshita M, Kuroda M. Heterochromatin protein 1gamma epigenetically regulates cell differentiation and exhibits potential as a therapeutic target for various types of cancers. Am J Pathol. 2009;174:309-16 pubmed publisher
Inoue A, Hyle J, Lechner M, Lahti J. Perturbation of HP1 localization and chromatin binding ability causes defects in sister-chromatid cohesion. Mutat Res. 2008;657:48-55 pubmed publisher
Wan L, Pan H, Hannenhalli S, Cheng Y, Ma J, Fedoriw A, et al. Maternal depletion of CTCF reveals multiple functions during oocyte and preimplantation embryo development. Development. 2008;135:2729-38 pubmed publisher
Zwerger M, Herrmann H, Gaines P, Olins A, Olins D. Granulocytic nuclear differentiation of lamin B receptor-deficient mouse EPRO cells. Exp Hematol. 2008;36:977-87 pubmed publisher
Zheng J, Xia X, Ding H, Yan A, Hu S, Gong X, et al. Erasure of the paternal transcription program during spermiogenesis: the first step in the reprogramming of sperm chromatin for zygotic development. Dev Dyn. 2008;237:1463-76 pubmed publisher
Wu L, Wang X, Li L, Zhao Y, Lu S, Yu Y, et al. Histone deacetylase inhibitor depsipeptide activates silenced genes through decreasing both CpG and H3K9 methylation on the promoter. Mol Cell Biol. 2008;28:3219-35 pubmed publisher
Poleshko A, Palagin I, Zhang R, Boimel P, Castagna C, Adams P, et al. Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response. J Virol. 2008;82:2313-23 pubmed
Takebayashi S, Tamura T, Matsuoka C, Okano M. Major and essential role for the DNA methylation mark in mouse embryogenesis and stable association of DNMT1 with newly replicated regions. Mol Cell Biol. 2007;27:8243-58 pubmed
Probst A, Santos F, Reik W, Almouzni G, Dean W. Structural differences in centromeric heterochromatin are spatially reconciled on fertilisation in the mouse zygote. Chromosoma. 2007;116:403-15 pubmed
Kobayakawa S, Miike K, Nakao M, Abe K. Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells. 2007;12:447-60 pubmed
Yang G, Rosen D, Zhang Z, Bast R, Mills G, Colacino J, et al. The chemokine growth-regulated oncogene 1 (Gro-1) links RAS signaling to the senescence of stromal fibroblasts and ovarian tumorigenesis. Proc Natl Acad Sci U S A. 2006;103:16472-7 pubmed
Norwood L, Moss T, Margaryan N, Cook S, Wright L, Seftor E, et al. A requirement for dimerization of HP1Hsalpha in suppression of breast cancer invasion. J Biol Chem. 2006;281:18668-76 pubmed
Olins D, Olins A. Granulocyte heterochromatin: defining the epigenome. BMC Cell Biol. 2005;6:39 pubmed
Santos F, Peters A, Otte A, Reik W, Dean W. Dynamic chromatin modifications characterise the first cell cycle in mouse embryos. Dev Biol. 2005;280:225-36 pubmed
product information
Catalog Number :
MAB3448
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P83917
Host Name :
Mouse
Antigen :
Heterochromatin Protein-1 β
Clone :
1MOD-1A9
Conjugate :
Ascites
Isotype :
IgG1
Product Description :
Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
Cross Reactivity :
Human;Mouse
Background :
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
ALT Names :
HP1b
Immunogen :
Recombinant mouse HP1beta.
Specificity :
Reacts with Heterochromatin protein-1 b (HP1b).
Package Size :
100 μL
Uses :
ELISA;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting
Storage :
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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