Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 | EMD Millipore MAB3446 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

catalog :

MAB3446

quantity :

100 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2HP-2G9

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig s2b	Natisvili T, Yandim C, Silva R, Emanuelli G, Krueger F, Nageshwaran S, et al. Transcriptional Activation of Pericentromeric Satellite Repeats and Disruption of Centromeric Clustering upon Proteasome Inhibition. PLoS ONE. 2016;11:e0165873 pubmed publisher
immunocytochemistry; human	Terada Y. Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition. Mol Biol Cell. 2006;17:3232-41 pubmed
	Yi Q, Chen Q, Yan H, Zhang M, Liang C, Xiang X, et al. Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion. J Biol Chem. 2019;294:2021-2035 pubmed publisher
	Keller K, Bhattacharya S, Borrás T, Brunner T, Chansangpetch S, Clark A, et al. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res. 2018;171:164-173 pubmed publisher
	Nguyen H, Nekanti U, Haus D, Funes G, Moreno D, Kamei N, et al. Induction of early neural precursors and derivation of tripotent neural stem cells from human pluripotent stem cells under xeno-free conditions. J Comp Neurol. 2014;522:2767-83 pubmed publisher
	Chakraborty A, Prasanth K, Prasanth S. Dynamic phosphorylation of HP1? regulates mitotic progression in human cells. Nat Commun. 2014;5:3445 pubmed publisher
	Lai Y, Song M, Hakala K, Weintraub S, Shiio Y. The interaction of the von Hippel-Lindau tumor suppressor and heterochromatin protein 1. Arch Biochem Biophys. 2012;518:103-10 pubmed publisher
	Hamilton C, Hayward R, Gilbert N. Global chromatin fibre compaction in response to DNA damage. Biochem Biophys Res Commun. 2011;414:820-5 pubmed publisher
	Gaudy Marqueste C, Roll P, Esteves Vieira V, Weiller P, Grob J, Cau P, et al. LBR mutation and nuclear envelope defects in a patient affected with Reynolds syndrome. J Med Genet. 2010;47:361-70 pubmed publisher
	Hayashihara K, Uchiyama S, Shimamoto S, Kobayashi S, Tomschik M, Wakamatsu H, et al. The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA. J Biol Chem. 2010;285:6498-507 pubmed publisher
	Britton S, Frit P, Biard D, Salles B, Calsou P. ARTEMIS nuclease facilitates apoptotic chromatin cleavage. Cancer Res. 2009;69:8120-6 pubmed publisher
	Takahashi A, Obata Y, Fukumoto Y, Nakayama Y, Kasahara K, Kuga T, et al. Nuclear localization of Src-family tyrosine kinases is required for growth factor-induced euchromatinization. Exp Cell Res. 2009;315:1117-41 pubmed publisher
	Poleshko A, Palagin I, Zhang R, Boimel P, Castagna C, Adams P, et al. Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response. J Virol. 2008;82:2313-23 pubmed
	Gilbert N, Thomson I, Boyle S, Allan J, Ramsahoye B, Bickmore W. DNA methylation affects nuclear organization, histone modifications, and linker histone binding but not chromatin compaction. J Cell Biol. 2007;177:401-11 pubmed

product information

Catalog Number :

MAB3446

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P45973

Host Name :

Mouse

Antigen :

Heterochromatin Protein-1 α

Clone :

2HP-2G9

Conjugate :

Ascites

Isotype :

IgG1

Product Description :

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

Cross Reactivity :

Human;Mouse

Background :

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

ALT Names :

HP1a; Antigen p25; Chromobox Protein Homolog 5

Immunogen :

Recombinant mouse HP1alpha.

Specificity :

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

Package Size :

100 μL

Uses :

ELISA;Immunoprecipitation;Western Blotting

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.