BrdU Antibody, clone AH4H7-1 / 131-14871 | EMD Millipore MAB3424 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BrdU Antibody, clone AH4H7-1 / 131-14871

catalog :

MAB3424

quantity :

50 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AH4H7-1 / 131-14871

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 32

Reference
Vetreno R, Bohnsack J, Kusumo H, Liu W, Pandey S, Crews F. Neuroimmune and epigenetic involvement in adolescent binge ethanol-induced loss of basal forebrain cholinergic neurons: Restoration with voluntary exercise. Addict Biol. 2020;25:e12731 pubmed publisher
Zaqout S, Bessa P, Kramer N, Stoltenburg Didinger G, Kaindl A. CDK5RAP2 Is Required to Maintain the Germ Cell Pool during Embryonic Development. Stem Cell Reports. 2017;8:198-204 pubmed publisher
Beckervordersandforth R, Ebert B, Schäffner I, Moss J, Fiebig C, Shin J, et al. Role of Mitochondrial Metabolism in the Control of Early Lineage Progression and Aging Phenotypes in Adult Hippocampal Neurogenesis. Neuron. 2017;93:560-573.e6 pubmed publisher
Quintes S, Brinkmann B, Ebert M, Fröb F, Kungl T, Arlt F, et al. Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair. Nat Neurosci. 2016;19:1050-1059 pubmed publisher
Wen Z, Zeng W, Dai J, Zhou X, Yang C, Duan F, et al. Paravertebral fascial massage promotes brain development of neonatal rats via the insulin-like growth factor 1 pathway. Neural Regen Res. 2012;7:1185-91 pubmed publisher
Bressan M, Louie J, Mikawa T. Hemodynamic forces regulate developmental patterning of atrial conduction. PLoS ONE. 2014;9:e115207 pubmed publisher
Zhang G, Vidal Pizarro I, Swain G, Kang S, Selzer M. Neurogenesis in the lamprey central nervous system following spinal cord transection. J Comp Neurol. 2014;522:1316-32 pubmed publisher
McClain J, Morris S, Marshall S, Nixon K. Ectopic hippocampal neurogenesis in adolescent male rats following alcohol dependence. Addict Biol. 2014;19:687-99 pubmed publisher
Ehlers C, Liu W, Wills D, Crews F. Periadolescent ethanol vapor exposure persistently reduces measures of hippocampal neurogenesis that are associated with behavioral outcomes in adulthood. Neuroscience. 2013;244:1-15 pubmed publisher
Mu J, Ostrowski R, Soejima Y, Rolland W, Krafft P, Tang J, et al. Delayed hyperbaric oxygen therapy induces cell proliferation through stabilization of cAMP responsive element binding protein in the rat model of MCAo-induced ischemic brain injury. Neurobiol Dis. 2013;51:133-43 pubmed publisher
Li J, Jiang T, Hughes M, Wu P, Yu J, Widelitz R, et al. Progressive alopecia reveals decreasing stem cell activation probability during aging of mice with epidermal deletion of DNA methyltransferase 1. J Invest Dermatol. 2012;132:2681-90 pubmed publisher
Wirsching H, Kretz O, Morosan Puopolo G, Chernogorova P, Theiss C, Brand Saberi B. Thymosin ?4 induces folding of the developing optic tectum in the chicken (Gallus domesticus). J Comp Neurol. 2012;520:1650-62 pubmed publisher
Paunovic B, Deng X, Khomenko T, Ahluwalia A, Tolstanova G, Tarnawski A, et al. Molecular mechanisms of basic fibroblast growth factor effect on healing of ulcerative colitis in rats. J Pharmacol Exp Ther. 2011;339:430-7 pubmed publisher
Acharya M, Christie L, Lan M, Giedzinski E, Fike J, Rosi S, et al. Human neural stem cell transplantation ameliorates radiation-induced cognitive dysfunction. Cancer Res. 2011;71:4834-45 pubmed publisher
McClain J, Hayes D, Morris S, Nixon K. Adolescent binge alcohol exposure alters hippocampal progenitor cell proliferation in rats: effects on cell cycle kinetics. J Comp Neurol. 2011;519:2697-710 pubmed publisher
Wey A, Martinez Cerdeno V, Pleasure D, Knoepfler P. c- and N-myc regulate neural precursor cell fate, cell cycle, and metabolism to direct cerebellar development. Cerebellum. 2010;9:537-47 pubmed publisher
Wey A, Knoepfler P. c-myc and N-myc promote active stem cell metabolism and cycling as architects of the developing brain. Oncotarget. 2010;1:120-30 pubmed
Hansson A, Nixon K, Rimondini R, Damadzic R, Sommer W, ESKAY R, et al. Long-term suppression of forebrain neurogenesis and loss of neuronal progenitor cells following prolonged alcohol dependence in rats. Int J Neuropsychopharmacol. 2010;13:583-93 pubmed publisher
Catlow B, Badanich K, Sponaugle A, Rowe A, Song S, Rafalovich I, et al. Effects of MDMA ("ecstasy") during adolescence on place conditioning and hippocampal neurogenesis. Eur J Pharmacol. 2010;628:96-103 pubmed publisher
Liu W, Ye P, O Kusky J, D Ercole A. Type 1 insulin-like growth factor receptor signaling is essential for the development of the hippocampal formation and dentate gyrus. J Neurosci Res. 2009;87:2821-32 pubmed publisher
Capuco A, Evock Clover C, Minuti A, Wood D. In vivo expansion of the mammary stem/ progenitor cell population by xanthosine infusion. Exp Biol Med (Maywood). 2009;234:475-82 pubmed publisher
Appel D, Kershaw D, Smeets B, Yuan G, Fuss A, Frye B, et al. Recruitment of podocytes from glomerular parietal epithelial cells. J Am Soc Nephrol. 2009;20:333-43 pubmed publisher
He J, Overstreet D, Crews F. Abstinence from moderate alcohol self-administration alters progenitor cell proliferation and differentiation in multiple brain regions of male and female P rats. Alcohol Clin Exp Res. 2009;33:129-38 pubmed publisher
Capuco A. Identification of putative bovine mammary epithelial stem cells by their retention of labeled DNA strands. Exp Biol Med (Maywood). 2007;232:1381-90 pubmed
Sauerhöfer S, Yuan G, Braun G, Deinzer M, Neumaier M, Gretz N, et al. L-carnosine, a substrate of carnosinase-1, influences glucose metabolism. Diabetes. 2007;56:2425-32 pubmed
Wasser M, Chia W. The extrachromosomal East protein of Drosophila can associate with polytene chromosomes and regulate gene expression. PLoS ONE. 2007;2:e412 pubmed
Persohn E, Seewald W, Bauer J, Schreiber J. Cell proliferation measurement in cecum and colon of rats using scanned images and fully automated image analysis: validation of method. Exp Toxicol Pathol. 2007;58:411-8 pubmed
Farmer C, Petit H, Weiler H, Capuco A. Effects of dietary supplementation with flax during prepuberty on fatty acid profile, mammogenesis, and bone resorption in gilts. J Anim Sci. 2007;85:1675-86 pubmed
He J, Crews F. Neurogenesis decreases during brain maturation from adolescence to adulthood. Pharmacol Biochem Behav. 2007;86:327-33 pubmed
Crews F, Mdzinarishvili A, Kim D, He J, Nixon K. Neurogenesis in adolescent brain is potently inhibited by ethanol. Neuroscience. 2006;137:437-45 pubmed
He J, Nixon K, Shetty A, Crews F. Chronic alcohol exposure reduces hippocampal neurogenesis and dendritic growth of newborn neurons. Eur J Neurosci. 2005;21:2711-20 pubmed
Rask Andersen H, Bostrom M, Gerdin B, Kinnefors A, Nyberg G, Engstrand T, et al. Regeneration of human auditory nerve. In vitro/in video demonstration of neural progenitor cells in adult human and guinea pig spiral ganglion. Hear Res. 2005;203:180-91 pubmed

product information

Catalog Number :

MAB3424

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BrdU Antibody, clone AH4H7-1 / 131-14871

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

BrdU

Clone :

AH4H7-1 / 131-14871

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-BrdU Antibody, clone AH4H7-1 / 131-14871

Cross Reactivity :

All

Background :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985).

ALT Names :

BrdU

Immunogen :

Bromodeoxyuridine-bovine serum albumin concentrate

Specificity :

Binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromodeoxyuridine does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.

Package Size :

50 μg

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry

Storage :

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.