Histone Antibody, clone H11-4 | EMD Millipore MAB3422 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histone Antibody, clone H11-4

catalog :

MAB3422

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H11-4

reactivity :

Bordetella pertussis, human, mouse, fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly ; 1:200; loading ...; fig 3s1a	Mills W, Lee Y, Kochendoerfer A, Dunleavy E, Karpen G. RNA from a simple-tandem repeat is required for sperm maturation and male fertility in Drosophila melanogaster. elife. 2019;8: pubmed publisher
immunohistochemistry; Bordetella pertussis; loading ...; fig 7	Eby J, Gray M, Hewlett E. Cyclic AMP-mediated suppression of neutrophil extracellular trap formation and apoptosis by the Bordetella pertussis adenylate cyclase toxin. Infect Immun. 2014;82:5256-69 pubmed publisher
western blot; mouse; 1:100	Chasseigneaux S, Clamagirand C, Huguet L, Gorisse Hussonnois L, Rose C, Allinquant B. Cytoplasmic SET induces tau hyperphosphorylation through a decrease of methylated phosphatase 2A. BMC Neurosci. 2014;15:82 pubmed publisher
immunocytochemistry; human; 1:10,000; loading ...; fig 1e	Morshed M, Hlushchuk R, Simon D, Walls A, Obata Ninomiya K, Karasuyama H, et al. NADPH oxidase-independent formation of extracellular DNA traps by basophils. J Immunol. 2014;192:5314-23 pubmed publisher
immunocytochemistry; human	O Donoghue A, Jin Y, Knudsen G, Perera N, Jenne D, Murphy J, et al. Global substrate profiling of proteases in human neutrophil extracellular traps reveals consensus motif predominantly contributed by elastase. PLoS ONE. 2013;8:e75141 pubmed publisher
western blot; human; 1:1000	Ouyang S, Hsuchou H, Kastin A, Pan W. TNF stimulates nuclear export and secretion of IL-15 by acting on CRM1 and ARF6. PLoS ONE. 2013;8:e69356 pubmed publisher
	Vankuren N, Long M. Gene duplicates resolving sexual conflict rapidly evolved essential gametogenesis functions. Nat Ecol Evol. 2018;2:705-712 pubmed publisher
	Pang B, Swords W. Haemophilus parainfluenzae Strain ATCC 33392 Forms Biofilms In Vitro and during Experimental Otitis Media Infections. Infect Immun. 2017;85: pubmed publisher
	De Cremoux P, Dalvai M, N Doye O, Moutahir F, Rolland G, Chouchane Mlik O, et al. HDAC inhibition does not induce estrogen receptor in human triple-negative breast cancer cell lines and patient-derived xenografts. Breast Cancer Res Treat. 2015;149:81-9 pubmed publisher
	Shu H, Nakamura M, Siretskiy A, Borghi L, Moraes I, Wildhaber T, et al. Arabidopsis replacement histone variant H3.3 occupies promoters of regulated genes. Genome Biol. 2014;15:R62 pubmed publisher
	Caudal D, Jay T, Godsil B. Behavioral stress induces regionally-distinct shifts of brain mineralocorticoid and glucocorticoid receptor levels. Front Behav Neurosci. 2014;8:19 pubmed publisher
	Holowacz T, Alexson T, Coles B, Doble B, Kelly K, Woodgett J, et al. The responses of neural stem cells to the level of GSK-3 depend on the tissue of origin. Biol Open. 2013;2:812-21 pubmed publisher
	Richardson G, BREAULT D, Horrocks G, Cormack S, Hole N, Owens W. Telomerase expression in the mammalian heart. FASEB J. 2012;26:4832-40 pubmed publisher
	Banerji U, van Doorn L, Papadatos Pastos D, Kristeleit R, Debnam P, Tall M, et al. A phase I pharmacokinetic and pharmacodynamic study of CHR-3996, an oral class I selective histone deacetylase inhibitor in refractory solid tumors. Clin Cancer Res. 2012;18:2687-94 pubmed publisher
	Holowacz T, Huelsken J, Dufort D, van der Kooy D. Neural stem cells are increased after loss of ?-catenin, but neural progenitors undergo cell death. Eur J Neurosci. 2011;33:1366-75 pubmed publisher
	Juneau R, Pang B, Weimer K, Armbruster C, Swords W. Nontypeable Haemophilus influenzae initiates formation of neutrophil extracellular traps. Infect Immun. 2011;79:431-8 pubmed publisher
	Chen A, Guo L, Ostaszewski B, Selkoe D, LaVoie M. Aph-1 associates directly with full-length and C-terminal fragments of gamma-secretase substrates. J Biol Chem. 2010;285:11378-91 pubmed publisher
	Madiraju P, Pande S, Prentki M, Madiraju S. Mitochondrial acetylcarnitine provides acetyl groups for nuclear histone acetylation. Epigenetics. 2009;4:399-403 pubmed
	Escamilla Del Arenal M, Recillas Targa F. GATA-1 modulates the chromatin structure and activity of the chicken alpha-globin 3' enhancer. Mol Cell Biol. 2008;28:575-86 pubmed
	Ramos C, Rafikova E, Melikov K, Chernomordik L. Transmembrane proteins are not required for early stages of nuclear envelope assembly. Biochem J. 2006;400:393-400 pubmed

product information

Catalog Number :

MAB3422

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone Antibody, clone H11-4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

Histone

Clone :

H11-4

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Histone Antibody, clone H11-4

Cross Reactivity :

Mammals;Human;Bovine;Rat;Xenopus;Opossum;Hamster

Background :

There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. The five major classes of histones are H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1 and H5 are known as the linker histones. Histones feature a main globular domain and a long N terminal tail which are involved with the structure of the nucleosomes of the 'beads on a string' structure. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.

Immunogen :

Purified nuclear fractions of HeLa cells

Specificity :

The antibody binds specifically to an antigen determinant, which is present on all five histone proteins. The antibody recognizes the histones H1, H2A, H2B, H3 and H4 with comparable sensitivity.

Package Size :

100 µg

Uses :

Immunocytochemistry;Immunohistochemistry;Western Blotting

Storage :

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. The Iyophilized antibody is stable at 2–8°C. Store the reconstituted antibody in aliquots at -15 to -25°C for up to 12 months.Repeated freezing and thawing should be avoided.